Ultra-small functional nanocluster, nanoprobe and preparation method and application of nanoprobe
A nanoprobe and functionalization technology, applied in the field of nanomedicine, can solve problems such as low targeting efficiency, affecting imaging effect, and increasing particle size of nanomaterials, so as to improve targeting ability, high production yield, and low toxicity Effect
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Embodiment 1
[0024] Put 2ml of chloroauric acid solution (concentration: 24mM), 1ml of gadolinium chloride solution (48mM), and 24mL of glutathione solution (4mM) in a 100mL single-necked flask, and stir slowly (1000rpm ) after 20 min, the temperature was raised to 70° C. to form nanoclusters, and after 24 h of reaction, the supernatant was removed by centrifugation for 20 min, and washed three times with 10 mL deionized water to obtain nanoclusters.
[0025] 30 μL of nanoclusters (50 mg / mL), 10 μL of EDC (8 mg / mL) and 20 μL of NHS (8 mg) were mixed in 1 mL of phosphate buffer (PBS buffer, 1×, pH 7.4), shaken at 37°C for 20 minutes, and then added 5 μL of cNGR polypeptide (1 mg / mL) was reacted for 2 hours, purified by centrifugation and stored in PBS buffer (1×, pH 7.4) to obtain ultra-small functionalized nanoprobes.
[0026] The obtained nanoprobe was diluted to 0.5 mg / mL, and its ultraviolet absorption spectrum was detected by a UV-visible spectrophotometer, its particle size was detect...
Embodiment 2
[0031] Put 2ml of chloroauric acid solution (concentration: 24mM), 1ml of gadolinium chloride solution (120mM), 24mL of glutathione solution (4mM) in a 100mL single-necked flask, stir slowly (1000rpm) under the condition of water bath (30°C) ) After 20 min, the temperature was raised to 70 °C to form nanoclusters. After 24 hours of reaction, the supernatant was removed by centrifugation for 20 minutes, and washed three times with 10 mL of deionized water to obtain nanoclusters.
[0032] 30 μL of nanoclusters (50 mg / mL), 10 μL of EDC (8 mg / mL) and 20 μL of NHS (8 mg) were mixed in 1 mL of phosphate buffer (PBS buffer, 1×, pH 7.4), shaken at 37°C for 20 minutes, and then added 10 μL of cNGR polypeptide (1 mg / mL), continued to react for 2 h, purified by centrifugation and stored in PBS buffer (1×, pH 7.4). The particle size of the prepared nanoprobe is 6nm.
Embodiment example 3
[0034] Put 2ml of chloroauric acid solution (concentration: 24mM), 1ml of gadolinium chloride solution (120mM), 24mL of glutathione solution (5mM) in a 100mL single-necked flask, and stir slowly (1000rpm ) After 20 min, the temperature was raised to 70 °C to form nanoclusters. After 24 hours of reaction, the supernatant was removed by centrifugation for 20 minutes, and washed three times with 10 mL of deionized water to obtain nanoclusters.
[0035]30 μL of nanoclusters (50 mg / mL), 10 μL of EDC (8 mg / mL) and 20 μL of NHS (8 mg) were mixed in 1 mL of phosphate buffer (PBS buffer, 1×, pH 7.4), shaken at 37°C for 20 minutes, and then added 15 μL of cNGR polypeptide (1 mg / mL), continued to react for 2 h, purified by centrifugation and stored in PBS buffer (1×, pH 7.4). The particle size of the prepared nanoprobe is 3.8nm.
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