A kind of high-density fermentation and spray drying method of Bacillus subtilis

Abstract

The invention discloses a high-density fermentation and spray-drying method of Bacillus subtilis. The method is to insert the Bacillus subtilis seed liquid into a fermentation medium for fermentation and cultivation, and adopt two-stage temperature control and regulation and fed-batch fermentation technology The concentration of live bacteria of Bacillus subtilis is increased to obtain a high-density bacterial liquid; then a drying protective agent is added to the high-density bacterial liquid, and after mixing evenly, spray drying is carried out to obtain a dry powder preparation of Bacillus subtilis. The method has simple process, simple operation, easy control, low cost and is suitable for large-scale industrial production. The dry powder preparation prepared by the invention has the advantages of high concentration of viable bacteria, high spore rate, good reproductive property, etc., can effectively improve the micro-ecological environment of water body, purify water quality of water body, and protect water environment.

Description

technical field [0001] The invention belongs to the technical field of environmental microbial fermentation, and in particular relates to a high-density fermentation and spray drying method of Bacillus subtilis. Background technique [0002] With the continuous expansion of the scale of aquaculture production in my country, the degree of intensification of aquaculture is increasing year by year, and the water environment is also deteriorating. A large amount of residual bait, feces, and dead animals and plants accumulated in the breeding process sink into the water, aggravating the accumulation of harmful substances such as ammonia nitrogen, nitrate, nitrite, and sulfide in the water body, making the degree of eutrophication in the water body more and more serious. high. Limited by the purification and regulation ability of the water body itself, the polluted water body causes a serious imbalance in the water ecology, promotes the growth and reproduction of pathogenic bacte...

AI Technical Summary

Problems solved by technology

In the existing production methods of Bacillus subtilis preparations, the components of the fermentation medium are single, the process control is too simple, the concentration of viable bacteria obtained is low, and the conversion rate of spores is low

The existing drying technology generally adopts freeze-drying technology or low-temperature drying technology, and the bacterial liquid or bacterial body needs to be concentrated by centrifugation or filtration before drying, which has a long production cycle, which increases the complexity and uncertainty of the preparation process, and further As a result, the survival rate of bacteria in the bacterial powder preparation after drying is low, and the actual use effect is not ideal, which is not conducive to large-scale promotion and application

Spray drying method is a method of drying atomized materials with hot air. The process is simple, the operation is simple, and the cost is low. It is widely used in large-scale industrial production; however, the high temperature generated during the spray drying process will affect heat-sensitive materials. cause irreversible damage