A kind of technique for preparing and extracting L-glutamine

A glutamine and process technology, applied in the field of preparation and extraction of L-glutamine, can solve the problems of high acid-base consumption for elution and regeneration, large amount of exchange resin, and high product cost, so as to promote utilization and transshipment, and reduce bacteria The effect of improving the content of solids and impurities and the efficiency of acid production

Active Publication Date: 2020-12-29
XINJIANG FUFENG BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the separation and extraction of L-glutamine at home and abroad almost all adopt column chromatography with ion exchange resin as filler. Although large-scale preparation can be carried out, the product cost is high, and the amount of exchange resin is huge, and it is difficult to elute and regenerate acid-base. Too much consumption will cause serious environmental pollution. In addition, L-glutamine is easily converted into glutamic acid in a strong alkali and strong acid environment, resulting in a very low yield

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  • A kind of technique for preparing and extracting L-glutamine

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039] A process for preparing and extracting L-glutamine, comprising the steps of:

[0040] 1) Fermentation: Brevibacterium flavum ATCC14067 seed solution (1×10 8 cfu / ml) was inserted into the fermentation medium according to the inoculum size of 5%, and fermented continuously for 50 hours to obtain the L-glutamine fermentation broth; the temperature during the fermentation process was controlled at 30°C, the pH was controlled at 6.8, and the pH was controlled at 6.8 during the fermentation process. The glucose concentration is not lower than 15g / L;

[0041] Wherein the fermentation medium is (mass percentage): glucose 15%, corn steep liquor 2%, ammonium sulfate 1%, magnesium sulfate 0.3%, dipotassium hydrogen phosphate 0.2%, potassium dihydrogen phosphate 0.2%, sodium chloride 0.1%, sulfuric acid Manganese 0.001%, ferrous sulfate 0.001%, V B1 0.0001%, pH 6.8;

[0042]Utilize the inorganic ceramic membrane to filter the fermented liquid to obtain the filtrate A (the L-glu...

Embodiment 2

[0053] A process for preparing and extracting L-glutamine, comprising the steps of:

[0054] 1) Fermentation: Brevibacterium flavum ATCC14067 seed solution (1×10 8 cfu / ml) was inserted into the fermentation medium according to the inoculum size of 5-7%, and fermented continuously for 45 hours to obtain the L-glutamine fermentation liquid; the temperature during the fermentation process was controlled at 30°C, and the pH was controlled at 6.8. Control the glucose concentration in the medium to not less than 15g / L;

[0055] Wherein the fermentation medium is (mass percentage): glucose 15%, corn steep liquor 2%, ammonium sulfate 1%, magnesium sulfate 0.3%, dipotassium hydrogen phosphate 0.2%, potassium dihydrogen phosphate 0.2%, sodium chloride 0.1%, sulfuric acid Manganese 0.001%, ferrous sulfate 0.001%, V B1 0.0001%, pH 6.8;

[0056] Utilize the inorganic ceramic membrane to filter the fermented liquid to obtain the filtrate A (the L-glutamine content is 2.83%) and separate...

Embodiment 3

[0067] Taking Example 1-2 as an example, the content of glutamine in the filtrate B was detected respectively; at the same time, a control group was also set up, wherein, the control group 1 was: only dialysis culture treatment was carried out, tourmaline powder and heat treatment were not used, and the rest were the same as in the embodiment 1; Control group 2: After the fermentation is completed, carry out tourmaline powder and dialysis culture treatment, do not use heat treatment, the rest are the same as in Example 1; Control group 3: After the fermentation is completed, carry out heat treatment and dialysis culture treatment, do not use tourmaline powder treatment, the rest With embodiment 1; Detect the content of glutamine in each group filtrate B, concrete results are shown in Table 1:

[0068] Table 1

[0069]

[0070] Conclusion: As shown in Table 1, the dialysis culture treatment of the fermentation waste cells can still produce a relatively high concentration of ...

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Abstract

The invention belongs to the technical field of fermentation and in particular discloses a process for preparing and extracting L-glutamine. The process comprises the following steps: preparing L-glutamine feed liquid, performing ultrafiltration treatment, removing mycoprotein and other macromolecular substances, performing activated carbon decolorization, strongly acidic cation exchange, vacuum concentration, isoelectric point cooling crystallization, centrifugation and the like on ultrafiltrate so as to obtain a crude product; re-dissolving the crude product, and sequentially performing weakly acidic and weakly alkaline ion exchange resin impurity removal, vacuum concentration, isoelectric point cooling crystallization, centrifugation and drying, thereby obtaining the refined L-glutamine. According to the process in the invention, the fermentation efficiency of the L-glutamine is improved, the finished product has high purity and total yield, the process is simple and convenient in production technology, the usage amount of the exchange resin is obviously decreased, and the amount of acid and alkali is greatly reduced, so that wastewater discharge is reduced, and environmental protection is facilitated.

Description

technical field [0001] The invention relates to a process for preparing and extracting L-glutamine, belonging to the technical field of fermentation. Background technique [0002] L-Glutamine (L-Gln) is a conditionally essential amino acid, and it is also one of the 20 basic amino acids that constitute proteins. It can be converted from glucose in the body and is a non-essential amino acid for mammals. Studies in recent years have shown that L-glutamine is the most abundant amino acid in the body, accounting for more than 60% of all free amino acids. It is mainly stored in the brain, skeletal muscle and blood, and has many special functions. Clinically, L-glutamine is used in the treatment of alcoholism, listlessness, gastric and duodenal ulcers and other diseases. In addition, L-glutamine also has the functions of improving nervous function, improving memory impairment after cerebral hemorrhage, improving the body's immunity, promoting the intellectual development of child...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P13/14C12R1/13
CPCC12P13/14
Inventor 张宗华刘洁邱庆志庄会华冯世红沈伟伟
Owner XINJIANG FUFENG BIOTECH
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