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Soil cultivation method for cordyceps militaris bonsai

A cultivation method and a technology for Cordyceps militaris are applied in the field of soil cultivation of Cordyceps militaris bonsai, can solve problems such as a single soilless cultivation method of Cordyceps militaris, achieve high content of cordycepin and SOD, a simple preparation method, and reduce risk costs. Effect

Inactive Publication Date: 2018-07-20
CHANGDE YANDI BIOTECH LTD CO
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] The purpose of the present invention is to provide a new soil-based cultivation method for Cordyceps militaris bonsai, which solves the shortcomi

Method used

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  • Soil cultivation method for cordyceps militaris bonsai

Examples

Experimental program
Comparison scheme
Effect test

Example Embodiment

[0035] Example 1:

[0036] (1) Configure nutrient solution: 2% glucose, 0.5% peptone, 0.5% beef extract, 0.1% magnesium sulfate heptahydrate, 0.1% potassium dihydrogen phosphate, vitamin b 1 0.001%, vitamin b 12 0.001%, 96.798% water, just mix evenly;

[0037] (2) Dispense the nutrient solution into triangular flasks, autoclave for 20 minutes, take it out and cool to room temperature for later use;

[0038] (3) Take out the Cordyceps strain (the Cordyceps strain comes from the megaspore head variety in the strain library of Changde Yandi Biotechnology Co., Ltd.), inoculate the strain into the above nutrient solution in a sterile environment, and seal;

[0039] (4) Place the strain on a shaker, set the rotation speed to 120rpm, the temperature is 17°C, and culture in the shake flask for 96 hours;

[0040] (5) After cleaning the live insects, use 75% alcohol to disinfect them in a sterile environment, and pack them into sterilized culture bottles;

[0041] (6) Inoculating the strain obtain...

Example Embodiment

[0050] Example 2:

[0051] (1) Configure nutrient solution: 2% glucose, 0.5% peptone, 0.5% beef extract, 0.1% magnesium sulfate heptahydrate, 0.1% potassium dihydrogen phosphate, vitamin b 1 0.001%, vitamin b 12 0.001%, 96.798% water, just mix evenly;

[0052] (2) Dispense the nutrient solution into triangular flasks, autoclave for 30 minutes, take it out and cool it to room temperature for later use;

[0053] (3) Take out the Cordyceps strain (the Cordyceps strain comes from the microspore head variety in the strain library of Changde Yandi Biotechnology Co., Ltd.), inoculate the strain into the above nutrient solution in a sterile environment, and seal;

[0054] (4) Place the strain on the shaker, set the speed to 150rpm, the temperature is 25℃, and culture in the shake flask for 120 hours;

[0055] (5) After cleaning the live insects, use 75% alcohol to disinfect them in a sterile environment, and pack them into sterilized culture bottles;

[0056] (6) Inoculating the strain obtained ...

Example Embodiment

[0065] Example 3:

[0066] (1) Configure nutrient solution: 2% glucose, 0.5% peptone, 0.5% beef extract, 0.1% magnesium sulfate heptahydrate, 0.1% potassium dihydrogen phosphate, vitamin b 1 0.001%, vitamin b 12 0.001%, 96.798% water, just mix evenly;

[0067] (2) Dispense the nutrient solution into triangular flasks, autoclave for 25 minutes, take it out and cool to room temperature for later use;

[0068] (3) Take out the Cordyceps strain (the Cordyceps strain comes from the coral grass species in the strain library of Changde Yandi Biotechnology Co., Ltd.), inoculate the strain into the nutrient solution in a sterile environment, and seal;

[0069] (4) Place the strain on a shaker, set the rotation speed to 130rpm, the temperature is 20°C, and culture in the shake flask for 110 hours;

[0070] (5) After cleaning the live insects, use 75% alcohol to disinfect them in a sterile environment, and pack them into sterilized culture bottles;

[0071] (6) Inoculating the strain obtained in st...

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Abstract

The invention relates to a soil cultivation method for a cordyceps militaris bonsai. The method concretely comprises the following steps: inoculating a living silkworm chrysalis or other living worm bodies with a strain in a sterile environment, and performing cultivation according to a conventional culture method until cordyceps militaris hyphae overgrow the interior of the worm body; after the steps of illumination color changing, primordium growing, bud generating, sporocarp growing and the like, moving a complex of a cordyceps militaris sporocarp and the worm body into a beautiful bonsai;adding disinfected nutrient soil, and inserting wood strips or toothpicks for fixing; and through a period of cultivation, after the complex grows out a sporocarp, performing trimming to form the pleasing soil cultivation cordyceps militaris bonsai. The cordyceps militaris cultivated by the method provided by the invention not only can be used as a food material, but also can be used to prepare the bonsai for viewing; the method has low costs and a simple process; and the cordyceps militaris has a higher cordycepin and superoxide dismutase (SOD) content.

Description

technical field [0001] The invention relates to the technical field of bonsai preparation technology of medicinal fungi and soil cultivation technology, in particular to a soil cultivation method of Cordyceps militaris bonsai. Background technique [0002] Natural Cordyceps militaris infects live larvae with Cordyceps mycelium, absorbs the larvae’s nutrition, the larvae are filled with Cordyceps mycelium and die, and become stiff pupae (worms). Under suitable conditions, the mycelium of Cordyceps fungus grows in the stiff pupae Propagate into a sub-seat, and the sub-seat continues to grow into a rod-shaped fruiting body from the head or other parts, resembling grass in appearance, and its complex is called "Cordyceps militaris". [0003] Cordyceps militaris is a treasure of nature, because it is rich in biologically active substances such as cordycepin, cordycepic acid, cordyceps polysaccharide, SOD, etc., and its medicinal value is very significant. Cordycepin (3'-deoxyade...

Claims

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Application Information

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IPC IPC(8): A01G18/20A01G18/30A01G18/40A01G18/50C05G1/00C12N1/14C12R1/645
CPCC05B7/00C05G5/20C12N1/14C05D5/00C05F1/007C05F11/00C05F11/10
Inventor 阳武雄易思富杨子遥王卫周丰杨胜辉
Owner CHANGDE YANDI BIOTECH LTD CO
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