Triple PCR primers, method and kit for detecting Streptococcus suis type 2, Pasteurella multocida and Haemophilus parasuis
A technology of Haemophilus suis and a detection kit, which is applied in biochemical equipment and methods, recombinant DNA technology, microbial measurement/inspection, etc. It can solve the problems of difficult to accurately distinguish Streptococcus suis, complicated operation, and long time consumption , to achieve the effect of good reliability, good application prospect and strong specificity
Active Publication Date: 2021-08-27
广东惠华生物科技有限公司
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Problems solved by technology
However, these methods are complicated to operate, time-consuming and difficult to accurately distinguish Streptococcus suis type 2, Pasteurella multocida and Haemophilus parasuis
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[0037] The following description is a preferred embodiment of the present invention, it should be pointed out that for those skilled in the art, without departing from the principle of the present invention, some improvements and modifications can also be made, and these improvements and modifications are also considered Be the protection scope of the present invention.
[0038] The preferred embodiments of the present invention will be described in detail below with reference to the accompanying drawings. The experimental method that does not indicate specific condition in the embodiment, generally according to routine condition. Unless otherwise specified in the examples of the present invention, all reagents and consumables used are commercially available.
[0039] 1. Purification and cultivation of Streptococcus suis type 2, Pasteurella multocida and Haemophilus parasuis
[0040] 1) Separate and purify Streptococcus suis type 2 and Pasteurella multocida according to the ...
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Abstract
The invention provides triplex PCR primers, a detection method and a kit for detecting Streptococcus suis type 2, Pasteurella multocida and Haemophilus parasuis. The kit provided by the invention designs triple PCR primers according to the highly conserved specific sequences for detecting Streptococcus suis type 2, Pasteurella multocida and Haemophilus parasuis, and establishes a single-tube, simultaneous detection of Streptococcus suis 2 Type, Pasteurella multocida and Haemophilus parasuis detection method. The kit of the present invention has the characteristics of rapidity, simplicity, strong specificity, high sensitivity and good reliability, can simultaneously analyze a large number of samples, and can identify Streptococcus suis type 2, Pasteurella multocida and The three pathogenic bacteria of Haemophilus parasuis with similar clinical symptoms can accurately differentiate and diagnose the cause of pig infection, which is conducive to implementing the correct treatment plan for the disease, and has a good application prospect.
Description
technical field [0001] The invention belongs to the technical field of animal epidemic detection, and in particular relates to a triple PCR primer, a detection method and a kit for detecting Streptococcus suis type 2, Pasteurella multocida and Haemophilus parasuis. Background technique [0002] Streptococcus suis type 2: Streptococcus suis (SS) belongs to Gram-positive bacteria, aerobic or facultatively anaerobic, spherical or oval in shape, often arranged in pairs or in short chains, without buds Hugging, can form capsule. According to its capsular polysaccharide (CPS), SS can be divided into 35 serotypes, namely type 1 / 2 and types 1-34, among which S. suis type 2 is the most virulent and most widespread serotype. Mainly cause meningitis, arthritis, septicemia, endocarditis, encephalitis, abortion, polyserositis, bronchial pneumonia, suppurative lymphadenitis and respiratory diseases in pigs. Clinically, the onset is often sudden, with body temperature rising to 40°C-43°C...
Claims
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IPC IPC(8): C12Q1/686C12Q1/689C12Q1/14C12Q1/04C12N15/11
CPCC12Q1/686C12Q1/689C12Q2600/16C12Q2537/143
Inventor 贺东生熊景峰苏丹萍周霞陈敏鸿司广斌梁伟放李锦辉
Owner 广东惠华生物科技有限公司