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Asymmetric dimethylarginine high-efficiency detection method

A dimethylarginine and detection method technology, which is applied in the field of high-efficiency detection of asymmetric dimethylarginine, can solve complex operation, high cost, and no ADMA detection method of asymmetric dimethylarginine And other issues

Active Publication Date: 2018-07-27
HANGZHOU BAICHEN MEDICAL LAB CO LTD +1
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Problems solved by technology

[0004] At present, clinical methods for the determination of asymmetric dimethylarginine ADMA mainly include high-pressure liquid chromatography, enzyme-linked immunoassay, and immunoturbidimetric assay, etc., but high-pressure liquid chromatography has the problem of insufficient sensitivity. Both the combined immunoassay and the immunoturbidimetric assay need to configure specific kits, and there are problems of complicated operation, low throughput and high cost
[0005] All in all, there is no generally accepted clinical detection method for asymmetric dimethylarginine ADMA

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[0040] The following will clearly and completely describe the technical solutions in the embodiments of the present invention with reference to the accompanying drawings in the embodiments of the present invention. Obviously, the described embodiments are only some, not all, embodiments of the present invention. All other embodiments obtained by persons of ordinary skill in the art based on the embodiments of the present invention belong to the protection scope of the present invention.

[0041] Those skilled in the art should understand that in the disclosure of the present invention, the terms "vertical", "transverse", "upper", "lower", "front", "rear", "left", "right", " The orientation or positional relationship indicated by "vertical", "horizontal", "top", "bottom", "inner", "outer", etc. is based on the orientation or positional relationship shown in the drawings, which are only for the convenience of describing the present invention and The above terms should not be con...

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Abstract

The invention discloses an asymmetric dimethylarginine high-efficiency detection method. The method is suitable for detecting content of asymmetric dimethylarginine in a sample, and the method comprises the following steps: obtaining an asymmetric dimethylarginine quantitative criterion rectification curve; preparing a detection product; separating the asymmetric dimethylarginine by high-flux liquid chromatography; detecting the asymmetric dimethylarginine by tandem mass spectrometry; and obtaining the content of the asymmetric dimethylarginine. The method uses the high-flux liquid chromatography-tandem mass spectrometry for rapidly and efficiently detecting the content of the asymmetric dimethylarginine in a biological sample.

Description

technical field [0001] The invention belongs to the technical field of biomedicine, and specifically relates to a high-efficiency detection method for asymmetric dimethylarginine, wherein the high-efficiency detection method for asymmetric dimethylarginine is suitable for quickly and efficiently detecting asymmetric dimethylarginine in biological samples. content of methyl arginine. Background technique [0002] Asymmetrical dimethylarginine (ADMA) is a natural amino acid widely present in tissues and cells. It uses S-adenosylmethionine as a methyl donor and is transferred by methylated proteins. The enzyme (Proteinarginine methyltransferase, PRMT) catalyzes arginine residues in proteins or polypeptides, making these arginine residues methylated to form methylated proteins, and then hydrolyzed from methylated proteins under the action of proteolytic enzymes Come. It is excreted unchanged by the kidneys and is mostly metabolized by dimethylarginine dimethylaminohydrolase. ...

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02
CPCG01N30/02
Inventor 吴超超胡志飞刘鹏刘纪松江思高强彭军
Owner HANGZHOU BAICHEN MEDICAL LAB CO LTD
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