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A method of increasing the pitch and length of algae filaments to increase the carbon fixation rate of Spirulina growth

A technology of spirulina and spirulina platensis, applied in the direction of microorganism-based methods, biochemical equipment and methods, microorganisms, etc., can solve problems such as unclear, increasing the pitch and length of spirulina filaments

Active Publication Date: 2020-10-20
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, it is not clear how to optimize the selection of algae species and the growth conditions of culture to increase the pitch and length of Spirulina's filaments, and then improve the growth rate of carbon fixation and filtration and harvesting efficiency. Therefore, it is necessary to take effective measures to improve its growth and harvesting. benefit

Method used

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  • A method of increasing the pitch and length of algae filaments to increase the carbon fixation rate of Spirulina growth

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] Adjust culture conditions to control HCO in Spirulina platensis liquid 3 - Concentration increased from 1.0g / L to 2.5g / L, CO 3 2- The concentration increased from 7.5g / L to 10.5g / L, the light increased from 28000lux to 68000lux, and the temperature of the algae liquid increased from 28°C to 33°C, so that the pitch of Spirulina platensis increased from 40μm to 60μm. The length of algal filaments increases from 320 μm to 720 μm, thereby obtaining spirulina species with high filtration and harvesting efficiency. The spirulina species with increased pitch and length of algal filaments were cultivated on a large scale in the photobioreactor of the runway pool, and the culture conditions were optimized and controlled: the pH value of the algae liquid was 9.5, NO 3 - The concentration is 145mg / L, PO 4 3- The concentration is 38mg / L, and the incubation time is 72 hours.

[0024] Collect 1 liter of algae liquid sample cultured in the photobioreactor, filter it three times...

Embodiment 2

[0026] Adjust culture conditions to control HCO in Spirulina platensis liquid 3 - Concentration increased from 1.2g / L to 2.7g / L, CO 3 2- The concentration increased from 8.0g / L to 11.0g / L, the light increased from 30000lux to 70000lux, and the temperature of the algae liquid increased from 29℃ to 34℃, so that the pitch of Spirulina platensis increased from 45μm to 65μm. The length of algal filaments increases from 340 μm to 740 μm, thereby obtaining spirulina species with high filtration and harvesting efficiency. Spirulina with increased pitch and length of algal filaments was cultivated on a large scale in the photobioreactor of the runway pool, and the culture conditions were optimized and controlled: the pH value of the algae liquid was 10.0, NO 3 - The concentration is 150mg / L, PO 4 3- The concentration is 40mg / L, and the incubation time is 72 hours.

[0027] Collect 1 liter of algae liquid sample cultured in the photobioreactor, filter it three times with a filter...

Embodiment 3

[0029] Adjust culture conditions to control HCO in Spirulina platensis liquid 3 - Concentration increased from 1.5g / L to 3.0g / L, CO 32- The concentration increased from 8.5g / L to 11.5g / L, the light increased from 32000lux to 72000lux, and the temperature of the algae liquid increased from 30°C to 35°C, so that the pitch of Spirulina platensis increased from 50μm to 70μm. The length of algal filaments increases from 360 μm to 760 μm, thereby obtaining spirulina species with high filtration and harvesting efficiency. Spirulina with increased pitch and length of algae filaments was cultivated on a large scale in the photobioreactor of the runway pool, and the culture conditions were optimized and controlled: the pH value of the algae liquid was 10.5, NO 3 - The concentration is 155mg / L, PO 4 3- The concentration is 42mg / L, and the incubation time is 72 hours.

[0030] Collect 1 liter of algae liquid sample cultured in the photobioreactor, filter it three times with a filter...

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Abstract

The invention relates to a biomass-energy utilization technology and aims at providing a method for increasing the growth carbon-sequestration rate in spirulina by increasing the spiral distance and the length of spirulina filaments. The method comprises the following steps of: taking a strain of spirulina platensis, adopting a conventional method to culture the strain till the spiral distance ofthe spirulina filaments is 40-50mu m and the length of the spirulina filaments is 320-360mu m; adjusting the culture conditions, and finally obtaining the spirulina platensis with the spiral distanceof the spirulina filaments being 60-70mu m and the length of the spirulina filaments being 720-760mu m; expanding the culture scale in a photobioreactor of a raceway pond so as to improve the densityand the filtering and harvesting efficiency of biomass in spirulina solution. The method provided by the invention has the beneficial effects that the spiral distance of the spirulina filaments can beincreased, simultaneously the length of the spirulina filaments is increased, and the density of the biomass of the spirulina solution can be increased, so that the harvesting efficiency of the biomass and the growth carbon-sequestration rate of the spirulina platensis after the spirulina solution flows through a filtering device are greatly increased.

Description

technical field [0001] The invention relates to biomass energy utilization technology, in particular to a method for increasing the pitch and length of algae filaments and improving the growth and carbon fixation rate of spirulina. Background technique [0002] The pitch and length of spirulina filaments are closely related to the biomass density and the harvesting efficiency of the filter device. When the pitch is less than 39 μm and the length of the filaments is less than 300 μm, the biomass density of spirulina will decrease, and the filaments will pass through. The ingress of the filter screen into the effluent resulted in a reduction in harvesting efficiency, resulting in a 28% reduction in harvested biomass yield. Therefore, increasing the pitch and length of spirulina filaments can effectively improve the biomass density of spirulina and the efficiency of filtration and harvesting, which has important economic significance for actual production. The unique helical s...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C12R1/01
CPCC12N1/20
Inventor 程军岑可法刘建忠周俊虎杨卫娟王智化张彦威周志军何勇
Owner ZHEJIANG UNIV
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