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Method for rapidly accumulating microalgae grease through pH regulation and control

A microalgae oil and rapid technology, applied in the fields of bioengineering and bioenergy, can solve the problems of low carbon sequestration efficiency, inactivation of carbonic anhydrase, complicated operation, etc. The effect of high volume density

Inactive Publication Date: 2014-04-30
ZHEJIANG UNIV
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Problems solved by technology

[0003] At present, there are still some bottlenecks in the research field of carbon sequestration and oil production of microalgae: high concentration of CO 2 It will inhibit the growth of microalgae and reduce the biomass yield; high concentration of CO 2 It will inhibit the oil accumulation of microalgae and reduce the oil yield; traditional oil promotion methods such as nitrogen suppression are complicated to operate, consume a lot of energy, and are expensive
[0008] High concentration of CO in flue gas 2 Generally, it will inhibit carbonic anhydrase and cell carbon fixation metabolism of microalgae, and CO 2 The continuous increase of the concentration may lead to the inactivation of free carbonic anhydrase in the liquid phase, thereby hindering the CO 2 fixed
As a result, biomass growth is inhibited and carbon sequestration is generally less efficient

Method used

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  • Method for rapidly accumulating microalgae grease through pH regulation and control
  • Method for rapidly accumulating microalgae grease through pH regulation and control
  • Method for rapidly accumulating microalgae grease through pH regulation and control

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Embodiment 1

[0045] (1) Pre-cultivate 5L of Chlorella vulgaris liquid (Chlorella vulgaris, provided by the Key Laboratory of Marine Bioengineering of Ningbo University) in an airlift reactor with a volume of 7L. 25±1°C, 5000lux continuous culture for about 5 days to enter the stable growth period, the biomass density of Chlorella is 0.6g L -1 .

[0046] (2) will be filled with 0.6g L -1 , 5L chlorella reactor at 2L min -1 Flow switch inlet with CO 2 flue gas (CO 2 Content is 10%), 5000lux light intensity, 24h light, temperature maintained at 25±1℃. The nitrate content in the initial culture solution was determined to be around 5mM.

[0047] (3) in CO 2 Cultured under flue gas conditions for 5 days to enter the stationary phase, on the 7th day of culture will contain CO 2 The flue gas was changed to air (that is, after the gas path was switched), and the pH value and dissolved oxygen (DO) of the culture medium were monitored online, and the oil content in the algae was determined by ...

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Abstract

The invention discloses a method for rapidly accumulating microalgae grease through pH regulation and control. The method comprises the following steps: (1) preculturing microalgae of biomass, and culturing microalgae in a reactor till the microalgae reach the stable growth period; (2) culturing in CO2, namely, introducing a CO2 waste gas into the reactor to culture the microalgae obtained in step (1) till the microalgae reach the stable period, and continuously culturing for 2-3 days; and (3) rapidly accumulating microalgae grease, namely, stopping introducing CO2 gas into the reactor but introducing air till the pH value of a culture liquid is higher than 10. The method overcomes the problem that the microalgae grease content is not high under the condition of high concentration CO2, achieves rapid accumulation of the grease under the condition of a sufficient nitrogen source, and has the advantages of high biomass density, rapid grease accumulation, low operation cost, convenience, rapidness, small energy consumption and the like.

Description

technical field [0001] The invention relates to the fields of bioengineering and bioenergy, in particular to a p A method for regulating the rapid accumulation of oil in microalgae. Background technique [0002] The increase of greenhouse gases in the atmosphere has led to global warming, and microalgae biological carbon sequestration technology is considered to be one of the effective methods to solve this problem. Microalgae biotechnology can also promote the accumulation of intracellular oil content through the control of culture conditions. It has the characteristics of fast growth and high oil content, so it is an important source of raw materials for biodiesel. [0003] At present, there are still some bottlenecks in the research field of carbon sequestration and oil production of microalgae: high concentration of CO 2 It will inhibit the growth of microalgae and reduce the biomass yield; high concentration of CO 2 It will inhibit the oil accumulation of microalgae ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P7/64C12R1/89
Inventor 程丽华姜加伟周伟康徐新华陈欢林
Owner ZHEJIANG UNIV
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