Method for preparing low temperature-resistant garbage treatment composite microbial agent

A compound bacterial agent and garbage treatment technology, which is applied in the field of microorganisms, can solve the problems of slow microbial growth, reduced garbage decomposition efficiency, and restrictions on the application of ordinary microbial agents, and achieves the effect of high sedimentation rate

Inactive Publication Date: 2018-08-14
SOUTHWEST BRANCH OF NORTHWEST RES INST CO LTD OF C R E C
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Northwest China is deep inland, the climate is dry and cold, and the winter is long. The months of the year with the highest average daily temperature below 20°C reach 8 months, and the freezing period is more than 3 months. Microorganisms grow slowly in low temperature conditions. Even stagnant, the decomposition efficiency of garbage will be greatly reduced, which limits the application of common microbial agents in solid waste treatment in this area

Method used

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  • Method for preparing low temperature-resistant garbage treatment composite microbial agent
  • Method for preparing low temperature-resistant garbage treatment composite microbial agent
  • Method for preparing low temperature-resistant garbage treatment composite microbial agent

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Experimental program
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Effect test

Embodiment 1

[0049] A preparation of a low-temperature-resistant garbage treatment composite bacterial agent, comprising the following steps:

[0050] (1) Strain collection

[0051] Collect 9 samples of garbage, landfill leachate and leachate-contaminated soil below the winter frozen layer in Qinghai garbage dump, each 500-1000g, take 5g from each sample, and transfer them to a triangular flask filled with 50mL sterile water Shake in medium for 5min to make 10 -1 Sample diluent, then dilute with sterilized water, configure 10 in sequence -2 、10 -3 、10 -4 、10 -5 、10 -6 Concentration sample dilution, each sample is equipped with the above 6 concentrations, and a total of 6 × 9 dilutions are obtained;

[0052] (2) Separation of strains

[0053] Under aseptic operation, take 600 μL from each of the above dilutions and divide them into three equal parts, and inoculate them into beef extract peptone plate medium, Gaoshi No. 1 plate medium, Chase plate medium and carboxymethyl fiber respec...

Embodiment 2

[0063] Effects on landfill leachate treatment and cellulose decomposition at low temperature:

[0064] 1. The growth status of the composite bacterial agent at low temperature

[0065] In order to compare the effects of low-temperature acclimation compound bacteria agents, we purchased two commercially available garbage treatment compound bacteria agents, commercially available agent A and commercially available agent B, and inoculated each agent in beef extract peptone liquid medium, shaking Cultivate until the bacterial concentration is 3.11-3.78×10 8 Inoculate in an Erlenmeyer flask containing 100ml enrichment medium at 10% by volume fraction, culture at 10°C with 130r / min shaking on a shaker, measure the absorbance value of the bacterial solution every 4h, and monitor the growth of the compound bacterial agent. And draw the growth curve of each group of compound bacterial agents. see results figure 1 .

[0066] The results showed that at a low temperature of 10°C, the gr...

Embodiment 3

[0074] The treatment effect of the compound bacterial agent in the outdoor simulation test:

[0075] Use a bioreactor to simulate the environment of the landfill site, fill the domestic waste into the bioreactor, and cultivate each group of compound bacterial agents to a bacterial concentration of 3.55-5.36×10 8 1 / ml, 10% volume fraction was added to landfill waste, and the same volume of sterile medium was added to the control group. During the test, the temperature of the garbage center, the concentration of ammonia nitrogen and COD in the leachate, and the sedimentation rate of the garbage were dynamically monitored.

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Abstract

The invention belongs to the technical field of microbes, and particularly relates to a method for preparing a low temperature-resistant garbage treatment composite microbial agent. The method comprises the following steps: strain collection, strain separation, strain enrichment, strain acclimation, primary strain screening, low temperature acclimation, and preparation of the composite microbial agent. The composite microbial agent prepared by the method of the invention is better than commercial common microbial agents in microbial agent growth, the removal rate of ammonia nitrogen and COD inlandfill leachate and the enzyme production activity of the cellulose at a low temperature of 10 DEG C; and outdoor simulated reactor test results show that the temperature of the center of the garbage rises fast, the concentrations of ammonia nitrogen and COD in the landfill leachate are low, and the sedimentation rate of the garbage after a reaction is high. The composite microbial agent is suitable for solid garbage treatment in northwest regions, and has a good application prospect.

Description

technical field [0001] The invention belongs to the technical field of microorganisms, and in particular relates to a preparation method of a low-temperature-resistant garbage treatment composite bacterial agent. Background technique [0002] Sanitary landfill is the main way to deal with domestic waste in Northwest my country. In recent years, with the gradual advancement of industrialization and urbanization, the production of domestic waste has increased year by year, and the problem of landfills has gradually become prominent, such as landfills Long stabilization time, continuous generation of landfill leachate with high pollution intensity, long-term emission of malodorous gas, etc. The study found that in the stabilization process of landfills, microorganisms play an important role and are the main decomposers of organic matter in the site, but under the influence of the complex environment of the landfill, only the indigenous microorganisms in the site The inefficienc...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12N1/14C12N1/36C12N1/02B09B3/00C12R1/07C12R1/13C12R1/38C12R1/885C12R1/66C12R1/465C12R1/01C12R1/645
CPCB09B3/00C12N1/02C12N1/14C12N1/20C12N1/36
Inventor 于贵李静柯佳闻苏凤舒中文
Owner SOUTHWEST BRANCH OF NORTHWEST RES INST CO LTD OF C R E C
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