Grouper fermentation Chinese herbal medicine feed additive capable of protecting liver as well as preparation method and application of grouper fermentation Chinese herbal medicine feed additive, and grouper feed
A technology for fermenting Chinese herbal medicines and feed additives, applied in applications, animal feeds, animal feeds, etc., can solve the problems of not being able to meet the needs of the aquaculture industry, toxic and side effects, and poor palatability, so as to improve immunity, promote proliferation, and reduce dosage Effect
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[0034] In addition, the embodiment of the present invention also provides a preparation method of the above-mentioned grouper liver-protecting fermented Chinese herbal medicine feed additive. For reference, the preparation method may include the following steps: preparing the Chinese herbal medicine mixture into a Chinese herbal medicine fermentation medium, and fermenting the Chinese herbal medicine The medium is inoculated with the seed liquid of Bacillus, and fermented.
[0035] The preparation of the Bacillus seed solution may include the following steps: inoculating the Bacillus in the seed medium, and then culturing at 28-32°C for 22-26h (preferably at 30°C for 24h).
[0036] The ingredients of the seed medium (in liquid form) include peptone, yeast extract and sodium salt. Among them, peptone and yeast extract mainly provide nitrogen sources for bacillus, and such nitrogen sources can also be replaced by soybean powder or ammonium sulfate. The carbon source in the seed...
Embodiment 1
[0053] (1) Prepare 10 g / L peptone, 5 g / L yeast extract, and 10 g / L NaCl to prepare a liquid seed medium with a pH of 7.0, sterilize at 121 °C for 30 min, and cool to room temperature before use.
[0054] (2) Pick 1 ring of Bacillus pumilus (Bacillus pumilus) preserved on the slant, inoculate it into the liquid seed medium prepared in step (1), and culture it on a shaking table at 30°C and 200r / min for 24h to obtain spores Bacillus seed solution is ready for use.
[0055] (3) with the ginkgo leaf of 8 weight parts, the licorice root of 18 weight parts, the artichoke of 5 weight parts, the milk thistle of 2 weight parts, the Ligustrum lucidum of 10 weight parts, the Artemisia argyi of 15 weight parts, the Astragalus, 8 parts by weight of Bupleurum, 3 parts by weight of Angelica sinensis, 10 parts by weight of turmeric, 3 parts by weight of Artemisia annua and 10 parts by weight of Schisandra chinensis are dried, pulverized, passed through an 80-mesh sieve, and evenly mixed to ob...
Embodiment 2
[0060] (1) Prepare 10g / L peptone, 5g / L yeast extract, and 10g / L NaCl to prepare a liquid seed medium with a pH of 6.9, sterilize at 121°C for 35min, and cool to room temperature before use.
[0061] (2) Pick 1 ring of Bacillus pumilus (Bacillus pumilus) preserved on the slant, inoculate it into the liquid seed medium prepared in step (1), and cultivate it on a shaking table at 28°C and 180r / min for 26h to obtain spores Bacillus seed solution is ready for use.
[0062] (3) with the ginkgo leaf of 8 weight parts, the licorice root of 18 weight parts, the artichoke of 5 weight parts, the milk thistle of 2 weight parts, the Ligustrum lucidum of 10 weight parts, the Artemisia argyi of 15 weight parts, the Radix Astragali, 8 parts by weight of Bupleurum, 3 parts by weight of Angelica sinensis, 10 parts by weight of Turmeric, 3 parts by weight of Artemisia caprifolia and 10 parts by weight of Schisandra chinensis are dried, pulverized, passed through a 100-mesh sieve, and evenly mixe...
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