Grouper fermentation Chinese herbal medicine feed additive capable of protecting liver as well as preparation method and application of grouper fermentation Chinese herbal medicine feed additive, and grouper feed

A technology for fermenting Chinese herbal medicines and feed additives, applied in applications, animal feeds, animal feeds, etc., can solve the problems of not being able to meet the needs of the aquaculture industry, toxic and side effects, and poor palatability, so as to improve immunity, promote proliferation, and reduce dosage Effect

Active Publication Date: 2018-08-17
JIMEI UNIV
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Problems solved by technology

But under the existing technical conditions, it also has many unresolved problems: (1) large dosage, slow effect; (2) poor palatabilit

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  • Grouper fermentation Chinese herbal medicine feed additive capable of protecting liver as well as preparation method and application of grouper fermentation Chinese herbal medicine feed additive, and grouper feed
  • Grouper fermentation Chinese herbal medicine feed additive capable of protecting liver as well as preparation method and application of grouper fermentation Chinese herbal medicine feed additive, and grouper feed
  • Grouper fermentation Chinese herbal medicine feed additive capable of protecting liver as well as preparation method and application of grouper fermentation Chinese herbal medicine feed additive, and grouper feed

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preparation example Construction

[0034] In addition, the embodiment of the present invention also provides a preparation method of the above-mentioned grouper liver-protecting fermented Chinese herbal medicine feed additive. For reference, the preparation method may include the following steps: preparing the Chinese herbal medicine mixture into a Chinese herbal medicine fermentation medium, and fermenting the Chinese herbal medicine The medium is inoculated with the seed liquid of Bacillus, and fermented.

[0035] The preparation of the Bacillus seed solution may include the following steps: inoculating the Bacillus in the seed medium, and then culturing at 28-32°C for 22-26h (preferably at 30°C for 24h).

[0036] The ingredients of the seed medium (in liquid form) include peptone, yeast extract and sodium salt. Among them, peptone and yeast extract mainly provide nitrogen sources for bacillus, and such nitrogen sources can also be replaced by soybean powder or ammonium sulfate. The carbon source in the seed...

Embodiment 1

[0053] (1) Prepare 10 g / L peptone, 5 g / L yeast extract, and 10 g / L NaCl to prepare a liquid seed medium with a pH of 7.0, sterilize at 121 °C for 30 min, and cool to room temperature before use.

[0054] (2) Pick 1 ring of Bacillus pumilus (Bacillus pumilus) preserved on the slant, inoculate it into the liquid seed medium prepared in step (1), and culture it on a shaking table at 30°C and 200r / min for 24h to obtain spores Bacillus seed solution is ready for use.

[0055] (3) with the ginkgo leaf of 8 weight parts, the licorice root of 18 weight parts, the artichoke of 5 weight parts, the milk thistle of 2 weight parts, the Ligustrum lucidum of 10 weight parts, the Artemisia argyi of 15 weight parts, the Astragalus, 8 parts by weight of Bupleurum, 3 parts by weight of Angelica sinensis, 10 parts by weight of turmeric, 3 parts by weight of Artemisia annua and 10 parts by weight of Schisandra chinensis are dried, pulverized, passed through an 80-mesh sieve, and evenly mixed to ob...

Embodiment 2

[0060] (1) Prepare 10g / L peptone, 5g / L yeast extract, and 10g / L NaCl to prepare a liquid seed medium with a pH of 6.9, sterilize at 121°C for 35min, and cool to room temperature before use.

[0061] (2) Pick 1 ring of Bacillus pumilus (Bacillus pumilus) preserved on the slant, inoculate it into the liquid seed medium prepared in step (1), and cultivate it on a shaking table at 28°C and 180r / min for 26h to obtain spores Bacillus seed solution is ready for use.

[0062] (3) with the ginkgo leaf of 8 weight parts, the licorice root of 18 weight parts, the artichoke of 5 weight parts, the milk thistle of 2 weight parts, the Ligustrum lucidum of 10 weight parts, the Artemisia argyi of 15 weight parts, the Radix Astragali, 8 parts by weight of Bupleurum, 3 parts by weight of Angelica sinensis, 10 parts by weight of Turmeric, 3 parts by weight of Artemisia caprifolia and 10 parts by weight of Schisandra chinensis are dried, pulverized, passed through a 100-mesh sieve, and evenly mixe...

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Abstract

The invention relates to a grouper fermentation Chinese herbal medicine feed additive capable of protecting the liver as well as a preparation method and application of the grouper fermentation Chinese herbal medicine feed additive, and a grouper feed, and belongs to the field of feeds. The feed additive comprises ginkgo leaves, licorice roots, artichoke, silybum marianum, glossy privet fruits, argy wormwood leaves, astragalus membranaceus, radix bupleuri, radix angelicae sinensis, turmeric, capillary wormwood herb, Chinese magnoliavine fruits and bacillus. The feed additive is safe and environmental-friendly and is free from toxic and side effects, and Chinese herbal medicines are high in drug effects and low in toxic and side effects. The preparation method comprises the following stepsof preparing a Chinese herbal medicine fermentation medium, then performing inoculating with seed liquid of bacillus and performing fermentation. The method is simple, the utilization rate of effective components of the Chinese herbal medicines can be increased, and bitter taste of the Chinese herbal medicines can be removed. The feed additive is applied to the grouper feed, so that grouper livercell and tissue damage caused by various reasons can be effectively prevented, damage to breeding of groupers, caused by liver diseases can be reduced, and the breeding benefits are increased. The grouper feed containing the feed additive has the effects of improving the entire health and the production performance of the groupers.

Description

technical field [0001] The invention relates to the field of feed, and in particular to a grouper liver-protecting fermented Chinese herbal medicine feed additive, a preparation method and application thereof, and a grouper feed. Background technique [0002] In recent years, due to the continuous deepening of aquaculture intensification, the continuous deterioration of the aquaculture environment, and drug abuse, fish diseases have been rampant, which has impacted the production of aquaculture and caused immeasurable economic losses. As an important organ for metabolism, digestion, excretion and immunity in fish, the liver is damaged by various metabolic toxins, viruses, bacteria, etc. in the deteriorating environment, which has aroused widespread concern in the aquaculture industry. [0003] In the past, the use of antibiotics, chemically synthesized drugs and steroid hormones was often used as an important way to treat or prevent fish liver diseases. However, a large amou...

Claims

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Application Information

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IPC IPC(8): A23K50/80A23K10/30A23K10/12
Inventor 宋凯鲁康乐王玲张东玲
Owner JIMEI UNIV
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