A culture medium for inducing spore production of Phytophthora capsici, preparation method and application

Abstract

The invention belongs to the technical field of culture mediums and discloses a culture medium of inducing phytophthora capsici to rapidly generate a large amount of zoosporangiums as well as a preparation method and application of the culture medium. The culture medium selects orange juice as a main raw material, and has a suitable pH value and abundant nutrition; the culture medium is prepared from a variety of inorganic and organic nutrients such as saccharides, protein, vitamin B1 and vitamin C which provide various nutrients required by the growth of the phytophthora capsici, so that theformation of phytophthora capsici sporangia is facilitated; by utilizing the culture medium, a large amount of the zoosporangiums can be induced within a short period; the experiment proves that the sclertium per square centimeter in 24h can generate as high as 31.9 thousands of the zoosporangiums, so that the problem that a general solid medium difficultly generates a large amount of the sporangia is solved and the difficult problem that the zoosporangiums generation by induction through a hydroponic method are easily polluted is also avoided.

Description

technical field [0001] The invention belongs to the technical field of culture medium, and relates to a culture medium for inducing spore production of Phytophthora capsici, more specifically, to a culture medium for inducing Phytophthora capsici to rapidly produce a large number of zoosporangia, a preparation method and an application thereof. Background technique [0002] Pepper blight (Phytophthora capsici) is a worldwide devastating disease caused by the infection of Phytophthora capsici Leonian. Phytophthora capsici belongs to the phytopathogenic oomycetes, and its pathogenic bacteria usually overwinter on the diseased plant residues, soil and seeds with oospores and chlamydospores, and become the source of primary infection of the disease in the next year. After the onset, the diseased part produces sporangia , sporangia are re-infected by wind, rain, irrigation and other agricultural activities, causing the spread and damage of the disease. In addition to harming pep...

AI Technical Summary

Problems solved by technology

The conventional method for Phytophthora capsici to produce sporangia is to use solid medium such as potato agar medium, corn flour medium, carrot medium, oat medium, etc. to cultivate under light for a certain period of time, or to add mycelium blocks to sterile water The hydroponic method of internally inducing sporangia. The former takes a long time to produce sporangia and it is difficult to obtain a large number of sporangia. Although the latter can quickly obtain a large number of zoosporangia, the operation steps are cumbersome and are easily contaminated by other bacteria during the operation.

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