Formula, manufacturing method and culture dish of culture medium

A culture medium and petri dish technology, applied in cell culture medium, culture process, tissue culture and other directions, can solve the problems of not allowing cell information to communicate and interact with each other, influence of experimental results, poor growth effect, etc., to achieve convenient research, Easy to operate, good effect of cultivation

Inactive Publication Date: 2018-08-17
ANQING MEDICAL COLLEGE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, these cell culture dishes have a common feature, that is, the absolute separation between the culture wells does not allow the mutual communication and interaction of cell information between different culture wells.
Its disadvantage is that if the sealed petri dish is opened every time, it may affect the experimental effect
[0003] The existing medium, the growth effect is not good, it is easy to cause misoperation and irreparable mistakes

Method used

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  • Formula, manufacturing method and culture dish of culture medium

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Embodiment 1

[0018]Embodiment 1 The formulation of a culture medium proposed by the present invention includes: L-cysteine ​​hydrochloride monohydrate, recombinant human insulin, basic fibroblast growth factor, L-proline, potassium chloride , D-(+)-glucose, thioglycerol, cell proliferation-promoting peptide, yeast powder, recombinant human transferrin, human serum albumin, IL-2 sustained-release microspheres, onion juice, IFN-γ sustained-release microspheres , riboflavin, vitamins, ginsenosides, D-glucose, folic acid, ferrous bile acid, gentamicin, sodium chloride, sodium bicarbonate, sodium dihydrogen phosphate, magnesium chloride, carotene, nuciferine, chloride Calcium, water-soluble vitamins, CD3-stimulating monoclonal antibody, human albumin, IL-2 sustained-release microspheres, L-leucine, Atractylodes polysaccharides, vitamin C, ginsenosides, cholesterol, lecithin, water-soluble non-polyelectrolytes Macromolecules, chlorophyll, anthocyanins, L-ascorbic acid and coagulation liquid.

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Abstract

The invention discloses a formula of a culture medium. The formula comprises L-cysteine hydrochloride monohydrate, recombinant human insulin, alkaline fibroblast growth factor, L-proline, L-leucine, atractylodes macrocephalaon polysaccharide, vitamin C, ginsenoside, cholesterol, lecithin, water-soluble non-polyelectrolyte polymer, chlorophyll, anthocyanin, L-ascorbic acid and agglomerated liquid.The formula of the culture medium has the advantages that the cost of the culture medium is low, the culture effect is good, not only can the growth requirement of bacteria be satisfied, but also thebright color of bacteria liquid of the bacteria can be ensured.

Description

technical field [0001] The invention relates to a formula of a culture medium, a preparation method and a petri dish. Background technique [0002] Cell culture technology is widely used in medical and biological research and applications. Typically, a cell culture dish is used to hold a liquid culture medium, and the cells grow adherently or in suspension in the liquid. Commonly used cell culture dishes can be divided into flat bottom and round bottom (U-shaped and V-shaped) according to the shape of the bottom; There are Terasaki plates and ordinary cell culture plates. The specific selection depends on the type of cultured cells, the required culture volume and different experimental purposes. However, these cell culture dishes have a common feature, that is, the absolute separation between the culture wells does not allow the mutual communication and interaction of cell information between different culture wells. Its disadvantage is that if the sealed petri dish is ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/00C12M1/38C12M1/36C12M1/34C12M1/22
CPCC12M23/10C12M23/38C12M31/10C12M41/12C12M41/26C12M41/48C12N5/0018C12N2500/24C12N2500/25C12N2500/32C12N2500/34C12N2500/36C12N2500/38C12N2500/44C12N2501/115C12N2501/2302C12N2501/24C12N2501/515C12N2501/90C12N2501/999
Inventor 李帆查诚张伟杨莹周明辉杨林青
Owner ANQING MEDICAL COLLEGE
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