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Wheat grain color related CAPS marker and detection method thereof

A wheat grain and labeling technology, applied in biochemical equipment and methods, recombinant DNA technology, microbial measurement/inspection, etc., can solve time-consuming and other problems

Active Publication Date: 2018-08-28
ANHUI AGRICULTURAL UNIVERSITY
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AI Technical Summary

Problems solved by technology

The former needs to construct a mapping population, which takes a long time, and the QTL is only limited to the difference loci in the two parents

Method used

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  • Wheat grain color related CAPS marker and detection method thereof
  • Wheat grain color related CAPS marker and detection method thereof
  • Wheat grain color related CAPS marker and detection method thereof

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Embodiment Construction

[0027] 1. Grain color determination:

[0028] The color of the seed coat was measured with a colorimeter (Minolta CR-5). The instrument uses the L*a*b color system to evaluate colors (CIE 1931). In this color system, the L value measures black (0)-white (100), when a is a positive value, it means red, otherwise it is green, when b is a positive value, it means yellow, otherwise it is blue. The brightness of the seed is also a factor that should be considered for the color of the seed coat, so the present invention uses a / L value to represent the color of the seed coat, and uses GC (grain color) to represent it. Weigh about 20 g of seeds and place them in a petri dish with a radius of about 25 mm in the instrument, repeat three times, and take the average value to represent the color phenotype value of each variety. In the Beijing 411 / Hongmangchun 21 population, the color phenotype of the grain was measured by the colorimeter in 2017, which was recorded as 17GrainColor-JH; in 20...

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Abstract

The invention discloses a wheat grain color related CAPS marker and a detection method thereof and relates to the technical field of molecular breeding of wheat. The CAPS marker is named as KU4104-6AL, and has a sequence of SEQ ID NO. 1. A method for identifying the wheat grain color comprises the following steps: 1, extracting DNA of to-be-detected wheat genome; 2, performing PCR (Polymerase Chain Reaction) amplification on the DNA of the wheat genome by utilizing sequences such as SEQ ID NO. 2 and SEQ ID NO.3 so as to obtain an amplification product; 3, performing digestion on the amplification product by utilizing a NruI incision enzyme having a recognition sequence of TCG / CGA so as to obtain enzyme-digested products; 4, ensuring that the color of corresponding wheat grains is dark whenthe enzyme-digested products are 214bp and 212bp, and naming genotype of the corresponding amplification product as KU4104-6A-a; ensuring that the color of corresponding wheat grains is light when the enzyme-digested product is 426bp. The CAPS marker KU4104-6AL developed by the method in the invention is capable of effectively distinguishing colors of the wheat grains and is used for genetic improvement of sprouting resistance of wheatears and flour quality.

Description

Technical field [0001] The invention relates to the technical field of wheat molecular breeding, in particular to a CAPS marker related to the color of wheat grains and a detection method thereof. Background technique [0002] The continuous rainy weather before wheat harvest may cause the physiologically mature grains to germinate directly on the wheat ears. This phenomenon is called Pre-harvest sprouting (PHS). Germination of wheat ears not only reduces yield, but also deteriorates quality (especially processing quality), affecting seed value. Most major wheat producing countries in the world suffer from ear germination, such as China, Canada, Australia, the United States, Japan, etc. Ear germination occurs more frequently in the middle and lower reaches of the Yangtze River, the southwest wheat region, the northeast spring wheat region, and the southern wheat region of Huanghuai ( Xiao et al. 2002; Liu et al. 2013; Zhou et al. 2017). [0003] Wheat grain color is closely relat...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6895C12N15/11
CPCC12Q1/6895C12Q2600/13C12Q2600/156
Inventor 常成闵晓宇张海萍姜昊曹佳佳未文新谢红咏张璨刘凯王建锋卢杰司红起马传喜
Owner ANHUI AGRICULTURAL UNIVERSITY
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