Cell separation device and method for using same

Abstract

A cell separation device configured for separating cells from micro-carriers or spheroids in a liquid is provided. The cell separation device includes a vessel comprising a first port, a second port,and a cavity; and a porous mesh disposed within the cavity to divide the cavity into a first compartment and a second compartment, wherein the first port is in communication with the first compartmentof the cavity, the first port located to a first side of the porous mesh, wherein the second port is in communication with the second compartment of the cavity, the second port located to a second side of the porous mesh, and wherein the porous mesh is positioned within the cavity to have a substantially vertical orientation or an inclined orientation with respect to a flow of liquid through theporous mesh.

Description

[0001] Cross References to Related Applications [0002] This application claims the benefit of priority to U.S. Provisional Application Serial No. 62 / 270,950, filed December 22, 2015, which is based upon the contents of that application and is hereby incorporated by reference in its entirety. technical field [0003] The present disclosure relates to cell separation devices and methods of using cell separation devices to separate cells from microcarriers or spheroids in a liquid, such as a cell culture medium. Background technique [0004] There are several known techniques for isolating cells from microcarriers in cell culture medium including, for example, differential gradient centrifugation, acoustic resonance, tangential flow filtration, spin filters, and sedimentation using conical or inclined plates. Most of these known techniques require expensive capital equipment and are complex to operate. Two less complex and commercially available devices for isolating cells f...

AI Technical Summary

Problems solved by technology

Understandably, the steps required make using the HyQ Harvestainer cumbersome

Images