Angiotensin-converting enzyme inhibitory peptide derived from Enteromorpha and its preparation method and application

Abstract

The invention discloses an angiotensin-converting enzyme inhibitory peptide derived from Enteromorpha enteromorpha and its preparation method and application, characterized in that the amino acid sequence of the inhibitory peptide is SEQ ID NO1: Phe-Gly-Met-Pro-Leu-Asp-Arg and / or SEQ ID NO 2: Met-Glu-Leu-Val-Leu-Arg, its preparation method includes the step of preparing Enteromorpha crude protein; adding trypsin enzymolysis to the Enteromorpha crude protein solution to obtain the enzymatic hydrolysis product; adding enzyme The hydrolysis product is subjected to gel chromatography after ultrafiltration separation, and the step of collecting the third absorption peak component containing ACE inhibitory peptide; the component containing ACE inhibitory peptide is subjected to gel chromatography, and then separated by high performance liquid chromatography , the 8th absorption peak component containing ACE inhibitory peptide is collected to obtain the inhibitory peptide, which has the advantages of strong hypotensive activity, high safety, no toxic side effects, and easy absorption by the human body.

Description

technical field [0001] The present invention relates to an angiotensin-converting enzyme inhibitory peptide and a preparation method thereof, in particular to a marine functional polypeptide with angiotensin-converting enzyme (ACE) inhibitory activity isolated and purified from the large marine green alga Enteromorpha and its preparation methods and applications. Background technique [0002] Angiotensin converting enzyme (ACE) is a zinc metalloprotease and one of the important proteases in the renin-angiotensin system. It plays an important role in the regulation of human blood pressure. By acting on the end of angiotensin I to remove His-Leu to generate angiotensin II, it can contract arterial smooth muscle and rapidly cause blood pressure to rise. Inhibiting ACE activity is an effective way to lower blood pressure. Most of the current drugs for the treatment of hypertension are chemical synthetic products, which have some adverse reactions, such as cough, taste disturba...

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Problems solved by technology

Ningbo University from the marine microalga Isoflagellates ( Isochrysis ) isolated and purified ACE antihypertensive peptide, its structure is Tyr--Met-Gly-Leu-Asp-Leu-Lys (patent name: an angiotensin-converting enzyme inhibitory peptide derived from marine microalgae, ZL201410172674.X ), but there are still relatively few ACE inhibitory peptides from seaweed, many of which cannot be further applied because the primary results of the peptides cannot be determined

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