A kind of separation and cultivation technology of sand shell insect

A technology of sand beetles and sand shells, which is applied in the field of microbiology, can solve problems such as difficult success and lack of sand beetle isolation and cultivation techniques

Active Publication Date: 2020-12-08
INST OF URBAN ENVIRONMENT CHINESE ACAD OF SCI
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  • Abstract
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  • Claims
  • Application Information

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Problems solved by technology

[0003] At present, there is still a lack of complete and standardized sandworm isolation and cultivation technology at home and abroad. Because sandworms have some unique requirements for their growth environment, and even only reproduce in high temperature seasons, conventional culture techniques are used to purify individuals isolated from the wild. Cultivation, generally difficult to succeed

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  • A kind of separation and cultivation technology of sand shell insect
  • A kind of separation and cultivation technology of sand shell insect
  • A kind of separation and cultivation technology of sand shell insect

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Embodiment

[0026] Isolation and Culture of Acanthus chinensis

[0027] 1) Field collection and enrichment of Acarina chinensis: Use 25# plankton net (64 μm pore size) to trawl the surface of the water body of Tingxi Reservoir in Xiamen City, Fujian Province for 10 minutes, 40 times. An enrichment sample was collected every 4-5 trawls, transferred to a sterile bottle for temporary storage, and shipped back to the laboratory within 4 hours.

[0028] 2) Isolation and purification of worm bodies: put the enriched Acanthus spp. on a petri dish or a concave glass slide, use a micropipette or a pipette gun (1-10μL) to clean the worm bodies under an inverted microscope or a dissecting microscope. Pick them one by one, soak them in ultrapure water for about 5 minutes, and rinse them 3-4 times.

[0029] 3) Starvation treatment of worm bodies: put the isolated and purified sand beetle worm bodies into a petri dish filled with ultrapure water, and starve them for 2-3 days at room temperature.

[0...

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Abstract

The invention discloses a difflugia separation and culture technique and belongs to the field of microbiology. The technique is mainly characterized in that single difflugia can be separated and purified from a natural water body, and indoor subculture can be implemented. A great amount of difflugia individuals can be obtained through indoor culture, obtaining time and space limits of the difflugia can be overcome, and rich experiment materials can be provided for deeply and systermatically studying difflugia. The technique is simple and efficient in method, low in cost and wide in applicationrange.

Description

technical field [0001] The invention belongs to the field of microbiology, in particular to the separation and cultivation technology of Difflugia, which can separate and purify a single Difflugia body from a natural water body, and carry out subculture. Background technique [0002] Testate amoebae are a common class of free-living unicellular eukaryotes, characterized by small size, high abundance, and high diversity, and play an important role in material cycle and energy flow in terrestrial and aquatic ecosystems. It has positive significance for maintaining ecological balance. Difflugia is a genus with the most species of shell insects. As a single-celled organism, sand beetle is not only an excellent material for studying genetics and evolution, but also sensitive to environmental ecological changes, and can indicate changes in climate, hydrology and other ecological factors. In recent years, with the in-depth research on sand shell insects, it is particularly import...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/10C12N1/02
CPCC12N1/02C12N1/10
Inventor 杨军王文平任可欣
Owner INST OF URBAN ENVIRONMENT CHINESE ACAD OF SCI
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