Cell separation machine temperature control system suitable for islet cell purification

Abstract

The invention discloses a cell separation machine temperature control system suitable for islet cell purification. The system comprises a cell separation mechanism, a cooling system and a machine boxprovided with a working cavity, wherein the cell separation mechanism comprises an actuator provided with a rotating shaft and further comprises a shaft sleeve, a centrifugal shaft, a centrifugal cupand a bearing which are arranged in the working cavity; the rotating shaft of the actuator is in linkage with the centrifugal shaft; and the cooling system comprises a cooling device, an upper radiator and a lower radiator. The cell separation machine temperature control system suitable for the islet cell purification has the advantages that the lower radiator is arranged, so the heat can be takenaway by the lower radiator during bearing working, thereby effectively preventing the heat generated during bearing working from being transmitted to the centrifugal cup, and the quality of the isletcell purification is effectively ensured; the upper radiator is arranged, the centrifugal cup can be effectively cooled, and it is ensured that the purification process can be controlled to be performed at a good temperature condition. The cell separation machine temperature control system suitable for the islet cell purification is used for the islet cell purification.

Description

technical field [0001] The invention relates to the field of medical technology, in particular to a temperature control system of a cell separator suitable for the purification of pancreatic islet cells. Background technique [0002] Islet cell transplantation is currently a very promising treatment for diabetes. Isolation and purification of sufficient, high-purity, and highly active islet cells from the pancreas is a key step in islet transplantation and the key to determining the success of islet transplantation. [0003] The isolation and purification steps of islets are relatively complicated. After the pancreas is perfused with collagenase for digestion, the islets are purified by continuous density gradient centrifugation, and then the extracted islets are evaluated for their status and safety, and are transplanted into the patient after meeting the safety standards. Purification of islet cells can increase the safety of transplantation, reduce immunogenicity and impr...

AI Technical Summary

Problems solved by technology

The room temperature that can be achieved in the usual laminar flow laboratory is 18°C. According to the proportional estimation, the possible temperature of the centrifuge cup at the room temperature of 18°C ​​is 24°C, which obviously cannot meet the equipment conditions of 4°C required for the separation and purification of islet cells.

Images