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Molecular markers closely linked to as98 dwarf traits in upland cotton and their applications

A technology of molecular markers and upland cotton, applied in the field of molecular biology, can solve the problem of not finding molecular markers

Active Publication Date: 2020-05-12
INST OF COTTON RES CHINESE ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Coarse mapping of dwarfing traits in AS98 was achieved by constructing segregating populations, but no molecular markers closely linked to dwarfing traits were found

Method used

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  • Molecular markers closely linked to as98 dwarf traits in upland cotton and their applications
  • Molecular markers closely linked to as98 dwarf traits in upland cotton and their applications
  • Molecular markers closely linked to as98 dwarf traits in upland cotton and their applications

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] (1) The dwarf variety AS98 of upland cotton was used as the male parent, and the normal tall upland cotton plant LHF10 was used as the female parent to obtain F 1 generation, and then self-crossed to obtain F containing 225 individual plants 2 .

[0024] (2) Extract parent, F 2 Genomic DNA of young leaves of a single plant in a population.

[0025] (3) The two parents AS98 and LHF10 were amplified with the designed 7 pairs of primers (Table 1). The amplification results showed that 14k-1, 14k-2 and 14k-3 of the 7 pairs of primers were amplified in AS98 The product band is single and there is no amplified product in LHF10 ( figure 2 ). Subsequent use of 14k-1 primers as molecular markers in F 2 Genotype analysis was carried out in the segregation population, and the results showed that the phenotypic traits of the individual plants that amplified the products were dwarf, and the phenotypic traits of the individual plants that failed to amplify the products were all...

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Abstract

The invention belongs to the field of molecular biology and discloses a molecular marker closely linked to dwarfing traits of upland cotton AS98 and application. The molecular marker is located on a D12 chromosome, a specific primer pair using the molecular marker can amplify 120 bp. products, a forward primer sequence of the specific primer pair is shown as SEQ ID No.1, and a reverse primer sequence of the specific primer pair is shown as SEQ ID No.2. A pair of primers for identifying mutants and wild types of the upland cotton dwarfing material AS98 are found out. 90% or more of phenotypic variations can be explained in an F2 population constructed by the upland cotton AS98 and a wild type LHF10. The molecular marker can be used for molecular marker-assisted selection and map cloning ofupland cotton AS98 dwarfing varieties, and a certain theoretical basis is provided for revealing cotton dwarfing mechanism and cultivating new dwarfing varieties.

Description

technical field [0001] The invention belongs to the field of molecular biology, and in particular relates to a molecular marker closely linked with the dwarfing trait of upland cotton AS98. Background technique [0002] Plant dwarfism is an important agronomic trait. Dwarf varieties have the characteristics of strong lodging resistance, suitable for close planting and mechanized management, which are of great significance for reducing labor intensity, improving fertilizer utilization rate and increasing yield. An important gene resource for studying plant stem growth and hormone regulation. [0003] Cotton has an infinite growth habit and the plants are tall. At present, in most cotton-producing countries in the world, the chemical inhibitor mepipeptide is widely used to control the growth of cotton, so that the plant height is generally controlled at about 90-100cm. Especially since the success of the "dense, short, early" planting technology in Xinjiang, the use of chem...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/6895C12N15/11
CPCC12Q1/6895C12Q2600/13
Inventor 杜雄明季高翔潘兆娥何守朴龚文芳贾银华王立如耿晓丽庞保印
Owner INST OF COTTON RES CHINESE ACAD OF AGRI SCI