Method for inducing initial B cells to differentiate into regulatory B cells in in-vitro way and culture conditions thereof

A technology for inducing differentiation and B cells, applied in cell culture active agents, biochemical equipment and methods, tissue culture, etc., to achieve the effect of improving survival rate, good application prospects, and improving the ability to induce differentiation
CN108588019AActive Publication Date: 2018-09-28BEIJING CHAOYANG HOSPITAL CAPITAL MEDICAL UNIV
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
BEIJING CHAOYANG HOSPITAL CAPITAL MEDICAL UNIV
Publication Date
2018-09-28

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Abstract

The invention discloses a method for inducing initial B cells to differentiate into regulatory B cells in an in-vitro way and culture conditions thereof. The method comprises the following step of performing induced culture on the initial B cells in an inducing culture medium, wherein the inducing culture medium consists of an RPMI-1640 culture medium, fetal calf serum, lipopolysaccharide, a CD40ligand, interleukin 1 beta, interleukin 6, interleukin 4, interleukin 21 and interleukin 23. The method has the advantages that the initial B cells are cultured by the inducing culture medium, so thatthe enough number of regulatory B cells (CD19<+>CD24<hi>CD27<+>) is obtained; the ability of inducing the initial B cells to differentiate into the regulatory B cells (CD19<+>CD24<hi>CD27<+>) is obviously improved, the survival rate of the regulatory B cells (CD19<+>CD24<hi>CD27<+>) after inducing differentiating is increased, and the good application prospect is obtained.
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Description

technical field

[0001] The invention belongs to the field of biological technology, and in particular relates to a method for inducing naive B cells to differentiate into regulatory B cells in vitro and culture conditions thereof. Background technique

[0002] Pleural effusion is a common clinical symptom characterized by pathological fluid accumulation in the pleural cavity. The pleural cavity is the potential gap between the visceral and parietal pleura. In normal people, there is 5-15 mL of fluid in the pleural cavity, which acts as a lubricant during respiratory movement. Pleural effusion occurs when fluid in the pleural space increases or decreases for any reason. There are a large number of immune cells in pleural effusion, such as lymphocytes, macrophages, granulocytes, and pleural mesothelial cells, among which B cells play an important immune role in pleural effusion. In the 1970s, scientists first described an immunosuppressive B cell, and many years later, the f...

Claims

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