Method and kit for differentiating mesenchymal stem cells into epidermis cells

A technology of mesenchymal stem cells and epidermal cells, applied in the field of a method and a kit for mesenchymal stem cells to differentiate into epidermal cells, can solve the problems of poor induction and differentiation ability of mesenchymal stem cells, unfavorable formation of epidermal cells, and non-specificity of differentiation To achieve the effect of improving the ability and specificity of inducing differentiation, increasing the speed of culture and proliferation, and reducing the time of inducing differentiation

Active Publication Date: 2017-07-07
华夏(青岛)生物科技有限公司
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Problems solved by technology

For example: the study of bone marrow mesenchymal stem cells induced to differentiate into epidermal stem cells in vitro (Long Jianhong, et al., Journal of Central South University, 2006, 06, 32-38) discloses a method for inducing bone marrow mesenchymal stem cells to differentiate into epidermal stem cells, The method is to add bone marrow mesenchymal stem cells into a differentiation-inducing culture medium for culturing, wherein the differentiation-inducing culture medium contains 20% fetal bovine serum, 8 μg / L EGF, and 50% HaCaT cell supernatant DMEM culture fluid; in addition, other growth factors, penicillins or antibiotics need to be added to the general differentiation-induced culture fluid. Poor, not conducive to the formation of epidermal cells, and the differentiation specificity is not strong

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  • Method and kit for differentiating mesenchymal stem cells into epidermis cells
  • Method and kit for differentiating mesenchymal stem cells into epidermis cells
  • Method and kit for differentiating mesenchymal stem cells into epidermis cells

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Embodiment 1

[0020] A method for differentiating mesenchymal stem cells into epidermal cells, the method comprising the steps of: adding mesenchymal stem cells into an inducing differentiation medium, at 37° C., 5% CO 2 In an incubator with saturated humidity, induce differentiation; the induced differentiation solution is mainly configured from a differentiation composition and an aqueous DMEM medium solution; the volume of the aqueous DMEM medium solution used to configure 5 mg of the differentiation composition is 1 mL; the The concentration of the DMEM medium aqueous solution is 10 mg / mL, and the differentiation composition is mainly prepared from the following ingredients in parts by weight: 1 part of transferrin, 0.5 parts of vitamin C, 5 parts of quercetin, α-tocopherol 2 parts of succinic acid monoester, 10 parts of isoflavone, 1 part of hydrogenated lecithin and 2 parts of lysine.

Embodiment 2

[0022] A method for differentiating mesenchymal stem cells into epidermal cells, the method comprising the steps of: adding mesenchymal stem cells into an inducing differentiation medium, at 37° C., 5% CO 2 In an incubator with saturated humidity, induce differentiation; the induced differentiation solution is mainly configured from a differentiation composition and an aqueous DMEM medium solution; the volume of the aqueous DMEM medium solution used to configure 7.5 mg of the differentiation composition is 1 mL; The concentration of the aqueous DMEM medium solution is 15 mg / mL, and the differentiation composition is mainly prepared from the following components by weight: 1.5 parts of transferrin, 0.6 parts of vitamin C, 60 parts of quercetin, α-tocopherol 3 parts of succinic acid monoester, 12 parts of isoflavone, 1.5 parts of hydrogenated lecithin and 3 parts of lysine.

Embodiment 3

[0024] A method for differentiating mesenchymal stem cells into epidermal cells, the method comprising the steps of: adding mesenchymal stem cells into an inducing differentiation medium, at 37° C., 5% CO 2 In an incubator with saturated humidity, induce differentiation; the induced differentiation solution is mainly configured from a differentiation composition and an aqueous DMEM medium solution; the volume of the aqueous DMEM medium solution used to configure 10 mg of the differentiation composition is 1 mL; the The concentration of the DMEM medium aqueous solution is 25 mg / mL, and the differentiation composition is mainly prepared from the following components in parts by weight: 3 parts of transferrin, 1 part of vitamin C, 10 parts of quercetin, α-tocopheryl amber 5 parts of acid monoester, 15 parts of isoflavone, 3 parts of hydrogenated lecithin, 5 parts of lysine, 1 part of maltitol, 5 parts of potassium dihydrogen phosphate, 3 parts of agar and 3 parts of biotin.

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Abstract

The invention provides a method and kit for differentiating mesenchymal stem cells into epidermis cells. The method includes the steps that the mesenchymal stem cells are added into an induced differentiation solution, and induced differentiation is conducted in a culture box with the temperature of 37 DEG C and the CO2 saturation humidity of 5%, wherein the induced differentiation solution is mainly prepared from a differentiation compound and a DMEM aqueous solution. The epidermis cells in a sufficient number are obtained after induced differentiation of the mesenchymal stem cells rich in autologous source. The induced differentiation solution used in the method can remarkably improve the induced differentiation capacity and specificity in the process that the mesenchymal stem cells are differentiated into the epidermis cells, induced differentiation time is remarkably shortened, and the survival rate of the epidermis cells obtained after induced differentiation is increased.

Description

technical field [0001] The invention belongs to the technical field of cell differentiation, and in particular relates to a method and a kit for differentiating mesenchymal stem cells into epidermal cells. Background technique [0002] Epidermal cells have multi-differentiation potential and form skin appendages such as sweat glands, hair and hair follicles in addition to regenerating the multilayered epidermal structure of the skin. Therefore, epidermal cells are ideal seed cells for constructing tissue-engineered skin. However, it is difficult to obtain and purify epidermal cells, which hinders the application of epidermal cells as seed cells for tissue engineering skin. Mesenchymal stem cells also have multi-directional differentiation potential, and can differentiate into a variety of tissue cells under different induction conditions, such as muscle cells, osteoblasts, fat cells, liver cells, cardiomyocytes, lung cells, nerve cells, etc. Ideal seed cells for tissue eng...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/071C12N5/0775
CPCC12N5/0625C12N2500/05C12N2500/12C12N2500/24C12N2500/30C12N2500/32C12N2500/34C12N2500/35C12N2500/36C12N2500/38C12N2506/1346
Inventor 汤苏阳
Owner 华夏(青岛)生物科技有限公司
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