A kind of detection method of glyphosate

A detection method, glyphosate technology, which is applied in the field of food safety detection, can solve the problems of inability to realize real-time online rapid detection, achieve good application prospects and potential application value, cheap and easy-to-obtain raw materials, and reduce costs

Active Publication Date: 2020-08-04
CHONGQING UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] In view of the above-mentioned deficiencies in the prior art, the purpose of the present invention is to provide a detection method for glyphosate, which solves the problem that the existing detection method cannot realize real-time online rapid detection

Method used

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  • A kind of detection method of glyphosate
  • A kind of detection method of glyphosate
  • A kind of detection method of glyphosate

Examples

Experimental program
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Effect test

Embodiment 1

[0031] 1) Synthesis of coumarin:

[0032] Weigh 1.26g of 4-(diethylamino)salicylic acid, 1.93g of 4-hydroxy-6-methyl-2-pyrone and 0.5g of triethylbenzyl ammonium chloride into 20ml of ethanol, mix well , take it out after refluxing at 90°C for 4 hours, cool naturally to room temperature to obtain a crude product, recrystallize the crude product, dissolve it in absolute ethanol, and finally separate it by thin-layer chromatography, and use 90 % ethanol solution is dissolved in coumarin solution. Wherein the eluent in the thin-layer chromatography plate is dichloromethane and triethylamine with a volume ratio of 1:9.

[0033] 2) Take the coumarin solution, divide it into 9 parts on average, and add a certain amount of Cu to each part of the coumarin solution 2+ Solution to obtain the reaction solution, mix evenly, so that the Cu in the solution 2+ The final concentrations in the reaction solution were 0 μM, 5 μM, 10 μM, 15 μM, 20 μM, 25 μM, 30 μM, 40 μM and 50 μM, and finally...

Embodiment 2

[0036] 1) Synthesis of coumarin:

[0037] Weigh 1.26g of 4-(diethylamino)salicylic acid, 1.93g of 4-hydroxy-6-methyl-2-pyrone and 0.5g of triethylbenzyl ammonium chloride into 20ml of ethanol, mix well , take it out after refluxing at 90°C for 4 hours, cool naturally to room temperature to obtain a crude product, recrystallize the crude product, dissolve it in absolute ethanol, and finally separate it by thin-layer chromatography, and use 90 % ethanol solution is dissolved in coumarin solution. Wherein the eluent in the thin-layer chromatography plate is dichloromethane and triethylamine with a volume ratio of 1:9.

[0038] 2) Take the coumarin solution, divide it into 10 parts on average, each 2.7ml, add 75uL of Cu with a concentration of 25mM to each coumarin solution 2+ solution, and then add a certain amount of glyphosate standard solution to the above mixed solution to obtain the total reaction solution, so that the final concentration gradient of glyphosate in the tota...

Embodiment 3

[0041] Test method is the same as embodiment 2, wherein incubation time is 5min, and the ethanol solution that dissolves coumarin is variable, and wherein, the volume ratio of ethanol and water is respectively 7:3, 7.5:2.5, 8:2, 8.5:1.5, 9:1, 9.5:0.5, and 10:0. Such as Figure 4 shown.

[0042] from Figure 4 It can be seen that under the condition that other factors are the same, when the volume ratio of ethanol to water is 9:1, the fluorescence recovery intensity of coumarin reaches the maximum value.

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Abstract

The invention discloses a glyphosate detecting method. Green fluorescence of coumarin is quenched by the electron transfer effect on the basis of copper ions; carboxyl and phosphate groups on glyphosate and the copper ions can be chelated to form a compound, so that the fluorescence of coumarin is recovered. The detecting method has the advantages that in the glyphosate detecting process, the operation is simple; the detection specificity is high; the detection sensitivity is high; the response speed is high; the result is sensitive and reliable; the cost is low; the real-time online fast andspecific detection can be realized; the method can be used for simple and fast detection on glyphosate pesticide residues in tap water and rice. Good application prospects and potential application values are realized in the fields of detection and analysis.

Description

technical field [0001] The invention relates to the technical field of food safety detection, in particular to a detection method for glyphosate. Background technique [0002] Glyphosate, whose chemical name is N-(phosphonomethyl)aminoacetic acid, is the most widely used and produced broad-spectrum non-selective herbicide in the world. The toxicology of glyphosate is mainly through the inhibition of choline ester Enzyme activity leads to nervous system dysfunction, and cases of water pollution, vegetation destruction, and livestock poisoning caused by glyphosate are increasing year by year. For this reason, all countries in the world have strengthened the detection of glyphosate residues in food. my country's national standard "Maximum Residue Limits of Pesticides in Food" (GM2763-2005) and the Sanitation Standard for Drinking Water (GB5749-2006) both There are strict limits on maximum residue levels (MRL) in crops such as rice, wheat, corn, fruit, sugarcane and cottonseed o...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N21/64
CPCG01N21/6428G01N2021/6432
Inventor 侯长军杨奕霞霍丹群侯经洲刘自山
Owner CHONGQING UNIV
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