A method for improving the nucleus retention rate and high-quality pearl rate of Pinctada martensii
A technology of pearl oyster martensii and seed planting, which is applied in the fields of application, climate change adaptation, fish farming, etc., can solve problems affecting the enthusiasm and economic benefits of pearl farming, high mortality of pearl oysters, and deterioration of the breeding environment, etc. problem, to achieve the effect of inhibiting bacterial resistance, significant social benefits, and easy promotion
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Embodiment 1
[0020] Method for treating pinctada martensii nucleating tools, bead nuclei and cell pieces with disinfectant filtered seawater prepared with disinfectant
[0021] (1) Preparation of disinfection treatment solution: Heat 10,000 ml of natural seawater filtered through coarse sand and fine sand to 100°C for 3 minutes, then place it in a cool place to cool to room temperature; add 0.1 g of tetracycline per 100 ml of seawater Take 10 grams of tetracycline in 10,000 milliliters of filtered seawater, grind the weighed tetracycline tablets into powder, add them to sterilized seawater and shake to prepare a solution with a weight-to-volume ratio of 0.1%.
[0022] (2) Preparation of maintenance solution for small pieces of cells: add 0.1 gram of tetracycline, 0.5 gram of glucose and 0.3 gram of compound amino acid per 100 milliliters of water, weigh 10 grams of tetracycline in 10,000 milliliters of sterilized seawater and grind them into powder, 50 grams of glucose and 30 grams of comp...
Embodiment 2
[0031] The method of using physiological saline to prepare disinfectant to treat the nucleating tools, bead nuclei and cell pieces of Pinctada martensii
[0032] (1) Preparation of disinfection treatment solution: add 0.1 g of cefixime dispersible tablets per 100 ml of normal saline, use 8000 ml of medical 0.9% sodium chloride injection (normal saline), weigh 8 g of cefixime, grind Add it into normal saline in powder form and shake it evenly to form a solution with a weight-to-volume ratio of 0.1%.
[0033] (2) Preparation of maintenance solution for small cell pieces: add 0.1 g of cefixime, 0.7 g of glucose and 0.5 g of compound amino acid per 100 ml of water, weigh 8 g of cefixime in 8000 ml of normal saline, and grind it into powder, 56 g Glucose and 40 grams of compound amino acids were dissolved in normal saline and shaken evenly. The use time of the maintenance solution is less than 4 hours.
[0034] (3) Disinfection treatment of nuclear implantation tools: 30 minutes ...
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