Sialyloligosaccharide-platinum nanoparticle, preparation method and use thereof in immunodetection of influenza virus

A sialic acid oligosaccharide, platinum nanotechnology, applied in the preparation of sugar derivatives, chemical instruments and methods, sugar derivatives, etc., can solve problems such as poor stability of results required by experimental conditions

Active Publication Date: 2018-10-09
INST OF MICROBIOLOGY - CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, in this detection, platinum nanoparticles still need to use protein antibodies to bind to target molecules, which is difficult to solve the problems of relatively harsh experimental conditions and poor stability of results.

Method used

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  • Sialyloligosaccharide-platinum nanoparticle, preparation method and use thereof in immunodetection of influenza virus
  • Sialyloligosaccharide-platinum nanoparticle, preparation method and use thereof in immunodetection of influenza virus
  • Sialyloligosaccharide-platinum nanoparticle, preparation method and use thereof in immunodetection of influenza virus

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0185] Embodiment 1 has the preparation of the sialic acid oligosaccharide of connecting arm and connecting arm

[0186] According to Examples 1-9 of the inventor's prior application CN103554198A, sialooligosaccharides with linking arms, lactose with linking arms and corresponding linking arms (as shown in Table 1) were prepared.

Embodiment 2

[0187] The preparation of embodiment 2 platinum nanoparticles

[0188] Add 17 mL of deionized water (Millipore membrane filtration), 2 mL of 7.72 mM chloroplatinic acid solution, and 0.5 mL of 10 mM sodium citrate solution into a 100 mL round bottom flask (soaked in aqua regia for 30 min), and stir at room temperature for 5 min. Quickly add 0.25mL 100mM sodium borohydride solution and stir for 15min. Stirring was stopped and allowed to stand at room temperature for 2 hours. Pass through a membrane and store at 4°C until use.

[0189] The as-prepared platinum nanoparticles were imaged by transmission electron microscopy, and the results were as follows: image 3 shown. The electron microscope model used is JEOL 1400.

[0190] Carry out XRD measurement to the prepared platinum nanoparticle, the result is as follows Figure 4 shown. The average diameter of the obtained platinum nanoparticles was calculated to be 2.5±0.2 nm.

Embodiment 3

[0191] Example 3 Preparation of sialooligosaccharide-platinum nanoparticles and lactose platinum nanoparticles

[0192] Take 2 milliliters of platinum nanoparticles prepared in Example 2 (average diameter is 2.5 ± 0.2nm, final concentration is 1.4 μ M), add 0.6-0.7 mg of any sialooligosaccharide with linking arm prepared in Example 1 or Lactose with linking arm and corresponding linking arm, make the molar ratio of the sialic acid oligosaccharide with linking arm or the lactose with linking arm and platinum nanoparticle be 110:1, and the sialic acid oligosaccharide with linking arm The ratio of sugar or lactose with linking arm to the corresponding linking arm was 5:1, and stirred at room temperature for 24 hours to obtain 9 kinds of sialooligosaccharide-platinum nanoparticles and 2 kinds of lactose platinum nanoparticles. After the reaction, wash twice with ultrapure water and once with PBS buffer (14000 rpm, 30 min). The product was dissolved in 1 milliliter of PBS buffer s...

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Abstract

The invention relates to a sialyloligosaccharide-platinum nanoparticle, a preparation method and use thereof in immunodetection of influenza virus. The sialyloligosaccharide-platinum nanoparticle is obtained by connecting sialyloligosaccharide having a linker arm to a surface of a platinum nanoparticle by an using S-Pt covalent bond. A immunodetection method in the invention completely avoids using antibodies and enzymes, and has advantages of high stability, easy preparation and acquisition and the like.

Description

technical field [0001] The invention relates to a sialic acid oligosaccharide-platinum nano particle and a preparation method thereof, as well as the application of the sialic acid oligosaccharide-platinum nano particle in immune detection of influenza virus. Background technique [0002] Influenza (flu) is a zoonotic infectious disease caused by influenza virus, and its host involves various animals such as people, pigs, birds, horses and dolphins. Studies have confirmed that the glycoprotein hemagglutinin (HA) on the surface of influenza virus can specifically recognize sugar chain receptors on the surface of host cells, which is the biological basis for influenza virus to infect the host, then replicate and continue to spread. Certain influenza virus variants have high pathogenicity and / or high mortality for multiple hosts, seriously threatening the health of animals and humans. Therefore, there is a need in the art for rapid and accurate detection of influenza virus. ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07H15/26C07H1/00G01N33/569G01N33/68G01N33/535
CPCC07H1/00C07H15/26G01N33/535G01N33/56983G01N33/68G01N2800/26
Inventor 李学兵郑隆堂张振兴吕迅
Owner INST OF MICROBIOLOGY - CHINESE ACAD OF SCI
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