LAMP quantitative detection kit for clostridium difficile tcdA virulent gene
A technology of Clostridium difficile and virulence genes, which is applied in the determination/inspection of microorganisms, DNA/RNA fragments, recombinant DNA technology, etc., can solve the problems of difficult strain typing, long detection time, insufficient sensitivity, etc., and achieve positioning Precise, high sensitivity, strong specific effect
Active Publication Date: 2018-10-12
厦门蓝特生物科技有限公司
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Some of these methods take a long time to detect, some are not sensitive enough and it is difficult to do strain typing
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[0035] In order to make the object, technical solution and advantages of the present invention clearer, the present invention will be further described in detail below in conjunction with specific embodiments. It should be understood that the specific embodiments described here are only used to explain the present invention, not to limit the present invention.
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The invention discloses a LAMP quantitative detection kit for a clostridium difficile tcdA virulent gene. An LAMP detection primer group of the clostridium difficile tcdA virulent gene, a qLAMP quantitative detection kit of the clostridium difficile tcdA virulent gene, and a method for quantitative detection of the clostridium difficile tcdA virulent gene are included. A tcdA gene LAMP detection technique and a real-time fluorescent quantitation technique are effectively combined (qLamp), the time is saved, specificity is high, sensitivity is high, and the purposes of tcdA virulent strain typing and quantitation can be achieved.
Description
technical field [0001] The invention belongs to the field of quantitative detection kits, in particular to a Clostridium difficile tcA LAMP Quantitative Detection Kit for Toxic Genes. Background technique [0002] Clostridium difficile was first discovered by Hall et al. in 1935 and got its name because it is strictly anaerobic and difficult to isolate and culture. Clostridium difficile is an conditionally pathogenic strain. When a patient takes antibiotics for a long period of time, the intestinal flora becomes unbalanced. At this time, Clostridium difficile proliferates in large numbers and releases a large amount of toxins, causing great harm to the human intestinal tract. damage. Studies have found that most Clostridium difficile produce two toxic proteins, exotoxin A and exotoxin B. Exotoxin A first binds to the toxin receptors of mucosal cells, destroying the intestinal mucosal barrier, causing intestinal inflammation and injury, and then exotoxin B further damages ...
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IPC IPC(8): C12Q1/689C12Q1/6844C12N15/11
CPCC12Q1/6844C12Q1/689C12Q2531/119C12Q2561/113C12Q2563/107C12Q2545/114Y02A50/30
Inventor 杨璐溪李晓燕
Owner 厦门蓝特生物科技有限公司