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A kind of multifunctional electrochemical sensor and its preparation method

A sensor and multi-functional technology, applied in the field of photoelectrochemistry, can solve the problems that cannot be achieved, and achieve the effects of easy control, efficient sterilization, good stability and reusability

Active Publication Date: 2020-05-01
INST OF OCEANOLOGY - CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the current photoelectrochemical sensors can only detect bacteria simply, and cannot have the multifunctional functions of simultaneous removal, detection and sterilization.

Method used

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  • A kind of multifunctional electrochemical sensor and its preparation method
  • A kind of multifunctional electrochemical sensor and its preparation method
  • A kind of multifunctional electrochemical sensor and its preparation method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039] Preparation method of multifunctional photoelectric sensor

[0040] Weigh the molar mass 2mmol, Bi(NO3 ) 3 ·5H 2 O and 0.1g PVP were added to 70mL ethylene glycol, stirred and dispersed for 3 hours, weighed 2mmol, KI was dissolved in 10mL, H 2 O, and then the two solutions were mixed evenly, and transferred to a 100mL Teflon reactor inner village. Immerse the pre-treated 304 stainless steel in the above mixed solution. Subsequently, the reaction kettle was heated to 160° C., and the reaction time was 4 hours. After the reaction is completed, the stainless steel material is taken out, rinsed with absolute ethanol and distilled water in sequence, and finally placed in a drying oven at 60°C for 5 hours to obtain a BiOI semiconductor thin film material on the surface of the stainless steel, and then the BiOI is immersed in 0.05% chlorine gold acid solution for 20 minutes, and then immersed in 10mM ascorbic acid solution for 30 minutes to obtain the AuNPs / BiOI film (see ...

Embodiment 2

[0042] Example 2: The multifunctional sensor obtained above is applied in a water body, and its detection performance on harmful microorganisms Escherichia coli.

[0043] Escherichia coli (100~10 6 ) Evaluation of multifunctional photoelectric sensors:

[0044] First prepare the bacterial suspension, inoculate the Escherichia coli stock solution into the sterilized LB liquid medium, and then place it in an air constant temperature shaker at 37°C and 150 rpm for overnight culture. After the cultured bacterial suspension was centrifuged, the bacterial suspension was diluted to 100-10 with sterile 0.01mol / L PBS (pH=7.4) buffer solution. 6 cfu / mL concentration gradient, and then immerse the multifunctional sensor prepared by the present invention in different concentrations of bacterial solution for 1 hour. Afterwards, the multifunctional sensor was washed and immersed in 100mM Na 2 SO 3 In the solution, a 500W xenon lamp was used as a light source for photoelectric signal mea...

Embodiment 3

[0046] Embodiment 3: the multifunctional photoelectric sensor of above-mentioned gained is to the killing of harmful microorganism Escherichia coli in water body:

[0047] Get gold Escherichia coli stock solution and dilute to 10 with sterilized 0.01mol / L PBS (pH=7.4) buffer solution 6 cfu / mL. Add 200 microliters of the diluted bacterial solution dropwise to the surface of the multifunctional sensor prepared in Example 1, take it after 30 minutes of light reaction, and dilute several times in sequence with 0.01mol / L PBS (pH=7.4) buffer solution according to the serial dilution method. gradient (10, 100, 100 times), and then take 100 μL from the solution of different dilution times to the prepared LB solid medium, and smear the bacterial solution evenly on the LB medium. Invert the LB medium, put it into an electric thermostat incubator and incubate at 37°C for 24 hours, count the number of colonies grown on the medium, and obtain the bacterial concentration by counting the co...

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Abstract

The invention belongs to the field of photoelectrochemistry, and particularly relates to a multifunctional electrochemical sensor for simultaneous capture, sterilization and detection and a preparation method thereof. The sensor is a BiOI flower-like film loaded with AuNPs and 4-Mercaptophenylboronic acid; wherein the loading amount of the AuNPs is 0.001 mg / cm<2>-0.01 g / cm<2>; the loading amount of the 4-Mercaptophenylboronic acid is 0.0001 mM / cm<2>-0.01 mM / cm<2>. The preparation method of the multifunctional electrochemical sensor has the advantages of simple process, easy control and low cost, and prepares the multifunctional photoelectrochemical sensor capable of simultaneous capture, sterilization and detection. Through the photocatalytic effect of a semiconductor film, harmful microorganisms in a water body can be effectively killed, pathogen in the water body can be detected according to the change of photoelectric signals of the semiconductor film, and the multifunctional electrochemical sensor has a good application prospect in the field of water purification.

Description

technical field [0001] The invention belongs to the field of photoelectrochemistry, and in particular relates to a multifunctional electrochemical sensor capable of capturing, sterilizing and detecting simultaneously and a preparation method thereof. Background technique [0002] Every year, many people die from bacterial water pollution, such as "super bacteria" pollution and so on. If bacterial contamination can be diagnosed quickly and the bacteria killed quickly, the mortality rate of bacterial infection will be greatly reduced [1]. [0003] Photoelectrochemical biosensor is an analytical method based on photoactive semiconductor materials and biologically sensitive molecules. Because photoelectrochemical sensors have the advantages of high efficiency, simplicity, easy operation and low cost, they have been widely used in the analysis of DNA[2], ions[3] and bacteria[4]. However, the current photoelectrochemical sensors can only detect bacteria simply, and cannot have t...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N27/26
CPCG01N27/26
Inventor 杨治庆王毅张盾
Owner INST OF OCEANOLOGY - CHINESE ACAD OF SCI
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