Solid state organic fertilizer containing micromonospora fermentation extracting powder as well as preparation method and application of solid state organic fertilizer

A micromonospora and organic fertilizer technology, applied to the field of solid organic fertilizer containing micromonospora fermented extract powder and its preparation and application, to achieve the effects of improving fertility, improving crop quality, and promoting growth

Pending Publication Date: 2018-10-26
郑州豫科生物技术有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the Micromonospora fermentation industry is in a

Method used

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  • Solid state organic fertilizer containing micromonospora fermentation extracting powder as well as preparation method and application of solid state organic fertilizer
  • Solid state organic fertilizer containing micromonospora fermentation extracting powder as well as preparation method and application of solid state organic fertilizer
  • Solid state organic fertilizer containing micromonospora fermentation extracting powder as well as preparation method and application of solid state organic fertilizer

Examples

Experimental program
Comparison scheme
Effect test

Example Embodiment

[0019] Example 1

[0020] A solid organic fertilizer containing the micromonospora fermented extract powder is composed of the following raw materials in weight percentage: 70% micromonospora fermented extract powder, 3% agricultural potassium sulfate, and the rest is iso-VC fermented extract. The preparation method is to directly mix the raw materials.

[0021] The micromonospora fermented extract powder is prepared by the following steps:

[0022] 1) Fermentation broth preparation: culture spores in sandy soil for 7 days at 34°C to obtain female slant spores, continue to culture at 34°C for 7 days to obtain daughter slant spores, and culture slant spores at 34°C for 38 hours to obtain shake flask spores for the following three-stage fermentation ; Firstly, the shake flask spores were fermented at 34-36°C for 35 hours to obtain the first-level seed culture solution, and then continued to ferment at 34°C for 33 hours to obtain the second-level seed culture solution; finally, ...

Example Embodiment

[0027] Example 2

[0028] A solid organic fertilizer containing the micromonospora fermented extract powder is composed of the following raw materials in weight percentage: 70% micromonospora fermented extract powder, 3% agricultural potassium sulfate, and the rest is iso-VC fermented extract. The preparation method is to directly mix the raw materials.

[0029] The Micromonospora fermented extract powder differs from Example 1 in that in step 1), the raw materials for the fermentation medium are starch, glucose, soybean cake powder, peptone, soybean oil and amylase. During the cultivation process, respectively Supplement the material twice at 20h and 46h, add 0.2% sodium chloride and 1% fish peptone at 32h; in the third stage fermentation, the pressure used should not exceed 0.4Kg / cm 2 . In step 2), HCl is added to the fermentation broth to acidify and adjust the pH to 2.5; sodium hydroxide is added to neutralize the pH to 7.2.

Example Embodiment

[0030] Example 3

[0031] A solid organic fertilizer containing the micromonospora fermented extract powder is composed of the following raw materials in weight percentage: 70% micromonospora fermented extract powder, 3% agricultural potassium sulfate, and the rest is iso-VC fermented extract. The preparation method is to directly mix the raw materials.

[0032] The Micromonospora fermented extract powder differs from Example 1 in that in step 1), the raw material of the fermentation medium consists of starch, glucose, soybean cake powder, peptone, soybean oil and amylase. During the cultivation process, Replenish the material twice at 20h and 46h respectively, add 0.2% sodium chloride and 1% fish peptone at 32h; in the third stage fermentation, the pressure used should not exceed 0.4Kg / cm 2 . In step 2), HCl is added to the fermentation broth to acidify and adjust the pH to 2.5; sodium hydroxide is added to neutralize the pH to 7.2.

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Abstract

The invention discloses a solid state organic fertilizer containing micromonospora fermentation extracting powder as well as a preparation method and application of the solid state organic fertilizer.The solid state organic fertilizer contains the following raw materials by weight percent: 70%-80% of micromonospora fermentation extracting powder, 2%-3% of agricultural potassium sulfate and the balance of an iso-VC fermentation extract. According to the preparation method, the micromonospora fermentation extracting powder is prepared from a cell suspension through filtration, salting-out precipitation and sheet frame filtration and can be directly mixed with the raw materials of the organic fertilizer to prepare the organic fertilizer, and meanwhile, the micromonospora fermentation extracting powder contains nutrients such as micromonospora mycelium proteins and residual culture medium proteins, so that the soil fertility can be remarkably improved; and when the solid state organic fertilizer is applied to the production of green vegetables, the yield difference can reach an extremely remarkable level by statistical analysis.

Description

technical field [0001] The invention specifically relates to a solid organic fertilizer containing micromonospora fermented extract powder, a preparation method and application thereof. Background technique [0002] At present, the smog climate in my country is very serious, especially in the northern region where more than 200 days / year of smog occurs. The reason is that in recent decades, my country's rapid industrialization and urbanization have accumulated environmental problems in the process of human production and life, showing high energy consumption, high emissions, heavy pollution, overcapacity, unreasonable layout, and excessive energy consumption. Accompanied by the huge emissions of carbon dioxide, sulfur dioxide, nitrogen dioxide, and fine particulate matter PM2.5 haze components. [0003] However, in the preparation process of Qingda raw materials, Micromonas fermentation broth is inseparable. There is no complete comprehensive utilization research technology...

Claims

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Application Information

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IPC IPC(8): C05G1/00C12N1/20C12R1/29
CPCC05D1/02C12N1/20C05F11/08C05F11/10
Inventor 陈兴业陈绮铭张硌程智慧乔晓芳卢明华尚卫华唐秋艳陈卫红沈红丽苏文明王秀艳胡雪瑞
Owner 郑州豫科生物技术有限公司
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