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A method for genetic control of the tea-foot moth by utilizing the reproductive regulation of the endosymbiont wolbachia

A genetic control and technology of tea inchworm, applied in the field of gray tea inchworm control, can solve the problem of unsatisfactory control effect of gray tea inchworm, and achieve the effect of ecological restoration and enrichment of control methods

Active Publication Date: 2021-03-16
TEA RES INST CHINESE ACAD OF AGRI SCI
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Problems solved by technology

For example, when using Eo NPV virus was found in the process of controlling tea inchworm, Eo NPV virus has a good effect on the control of small tea inchworm; but the effect of control of gray tea inchworm is not ideal

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0018] (1) Species identification of tea geometrids in control plots

[0019] 1. Use the 5-point sampling method in the control plot to collect samples of tea geometrid larvae (the number of samples is not less than 20), soak them in absolute alcohol and store them, and bring them back to the laboratory for species identification using molecular biology methods. First, use the Tiangen kit to extract insect genomic DNA, and use genomic DNA as a template to amplify COI Fragment sequence, amplification primers are LepF1: (5'-ATTCAACCAATCATAAAGATATTGG-3'); LepR1 (5'-CTCCWCCAGCAGGATCAAAA-3'). The amplified products were sent to Qingke Biological Co., Ltd. for sequencing. After the sequencing results were spliced ​​by Seqman 5.01), the MEGA 5.0 software was used to compare and calculate the genetic distance. If the genetic distance was less than 2.5%, it was considered as the tea-foot moth. The standard sequence of C. cinereus was downloaded from GenBank, and the gene accession n...

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PUM

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Abstract

The invention discloses a method for performing genetic control on ectropis grisescens Warren by utilizing the reproductive regulation effect of endosymbiotic bacteria Wolbachia, and belongs to the technical field of ectropis grisescens Warren control methods. The method includes the steps of (1) control object authentication; (2) prediction of the occurrence quantity and occurrence period of ectropis grisescens Warren in a control field; (3) establishment of a pure strain of ectropis grisescens Warren without Wolbachia; (4) release of pupae or adults. The method is safe to the environment andthe tea quality and good in control effect.

Description

technical field [0001] The invention belongs to the technical field of prevention and control methods of gray tea inchworm, and in particular relates to a method of using endosymbiotic bacteria Wolbachia A method for genetic control of the reproductive regulation of Grifola cinerea. Background technique [0002] The gray tea-foot moth is the most important pest of tea trees in my country, mainly distributed in tea-producing provinces such as Fujian, Hunan, Hubei, Jiangxi, Anhui, Henan, Zhejiang, and Jiangsu. The gray tea-foot moth has 6 to 7 generations a year, and its larvae mainly feed on the leaves of tea trees to cause damage. When it occurs seriously, it will eat up all the leaves of the tea garden, often causing major economic losses to tea production. At present, there are many control methods about the gray tea inchworm, the most important of which is chemical control. Due to the repeated spraying and irrational use of chemical pesticides throughout the year, the r...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01G13/00A01K67/033
CPCA01G13/00A01K67/033
Inventor 王志博肖强白家赫周孝贵唐美君
Owner TEA RES INST CHINESE ACAD OF AGRI SCI
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