Model capable of real-timely observing growth of sugarcane tissue culture seedling root

A technology for root growth and tissue culture seedlings, which is applied in the field of real-time observation of the root growth model of sugarcane tissue culture seedlings, can solve problems such as the inability to observe root growth in real time, and achieves significant bacteriostatic effect, strong practicability, and simple operation.

Inactive Publication Date: 2018-11-06
FUJIAN AGRI & FORESTRY UNIV
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Problems solved by technology

In view of the problem that the root growth of sugarcane cannot be observed in real time during the existing sugarcane research process, the growth of the sugarcane root system can be observed in real time through the growth model of the sugarcane tissue culture root system in liquid culture, which facilitates the smooth development of sugarcane root research

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  • Model capable of real-timely observing growth of sugarcane tissue culture seedling root
  • Model capable of real-timely observing growth of sugarcane tissue culture seedling root

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Embodiment 1

[0033] Embodiment one: a kind of preparation method of real-time observation sugarcane tissue culture seedling root growth model, preparation steps are as follows:

[0034] 1. Configuration of nutrient solution:

[0035] The nutrient solution formula for cultivating the root growth of sugarcane tissue culture seedlings is: KNO 3 1000mg, NH 4 NO 3 1000mg, Mg(SO) 4 •7H 2 O 300mg, KH 2 PO 3 150mg, CaCl 2 • 2H 2 O 400mg, FeSO 4 •7H 2 O 28mg, Na2 -EDTA 37mg, MnSO 4 • 4H 2 O 20mg, ZnSO 4 •H 2 O 8mg, H 3 BO 3 2mg, 10% NaClO 40mL, Timentin 15 mg, 2(C 3 h 5 o 2 ) Ca (calcium propionate) 30mg, C 12 h 25 SO 3 Na (sodium dodecyl sulfonate) 0.2mg, add water to dissolve and dilute to 1000mL.

[0036] 2. Prepare the root growth model of sugarcane tissue culture seedlings:

[0037] A. Take a transparent rectangular container, which is equipped with a root support for the crop roots to absorb water and nutrients, and inject a nutrient solution with a volume concentra...

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Abstract

The invention relates to a model capable of real-timely observing the growth of sugarcane tissue culture seedling root. The preparation method comprises: preparing a nutrition solution and preparing asugarcane tissue culture seedling root growth model. According to the present invention, the prepared culture liquid has significant antibacterial effect; by using the nutrition solution, the sugarcane tissue culture seedling root growth process can be carried out under the condition close to the natural environment so as to achieve the real plant growth environment, the growth and development condition of the sugarcane tissue culture seedling root can be real-timely observed for 24 h, the data during the growth and development can be collected, the reliable data can be provided for the rootresearch, and the foundation can be established for the research on the sugarcane root; the equipment is simple, the operation is convenient, and the practicability is strong; and with the model, theproblem that the root growth condition cannot be real-timely observed in the long-term sugarcane root research is solved, and the condition is provided for the intuitive and real-time observation anddetection of the sugarcane tissue culture seedling root growth condition.

Description

technical field [0001] The invention relates to a crop root growth model, in particular to a real-time observation root growth model of sugarcane tissue culture seedlings, which provides scientific data for real-time detection of the root growth status of sugarcane tissue culture seedlings, and belongs to the field of biotechnology. Background technique [0002] sugar cane( Saccharum Complex) is the most important sugar crop in my country. Sucrose accounts for 80% of the world's total sugar production and more than 92% of my country's total sugar production. At the same time, sugarcane has high photosynthetic efficiency and is the C4 crop with the highest biological yield among cultivated crops. One of the best crops for producing green fuel ethanol. Sugarcane production has important practical significance and far-reaching impact on increasing farmers' income in sugarcane areas, adjusting industrial structure and ensuring national energy security. With the advancement of ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/008
Inventor 杨颖颖高世武许莉萍林庆良阙友雄苏亚春郭晋隆吴期滨唐柳青
Owner FUJIAN AGRI & FORESTRY UNIV
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