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Method for promoting rapid germination of paris polyphylla seeds

A kind of seed and rapid technology, applied in the direction of seed and rhizome treatment, application, agriculture, etc., can solve the problems of complicated operation and poor practicability, and achieve the effect of simple operation, universal applicability, and high germination rate

Inactive Publication Date: 2018-11-16
CHENGDU INST OF BIOLOGY CHINESE ACAD OF S
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This technology is more complicated in operation, and its practicality in operation is not very strong.

Method used

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  • Method for promoting rapid germination of paris polyphylla seeds
  • Method for promoting rapid germination of paris polyphylla seeds
  • Method for promoting rapid germination of paris polyphylla seeds

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039] Embodiment one: soaking frequency and time are preferred

[0040] 1. Select 1,900 seeds of Chonglou yunnanensis that were fresh and mature in the same year, uniform in size, in the same state, and stored for the same storage time, and divided them into 19 groups, with 100 seeds in each group. The weight of seeds was not significantly different within and between groups. Seed germination was performed using the method described above. Among them, the soaking time is " / ", indicating that the soaking has not been carried out; the concentration of sodium tungstate is 0.1%, the concentration of hydrogen peroxide is 2%, the concentration of potassium nitrate is 19mmol / L, and the concentration of gibberellin is 500mg / L , the concentration of mercury is 0.1%. The specific operating parameters of each group are shown in Table 1.

[0041] The specific preparation parameters of each group in table 1

[0042]

[0043] 2. Calculate the average germination rate of the above-men...

Embodiment 2

[0054] Embodiment two: preferred soaking sequence

[0055] 1. Select 1,100 seeds of Chonglou yunnanensis that were fresh and mature, uniform in size, in the same state, and stored for the same storage time in the same year, and divided them into 11 groups, with 100 seeds in each group. There was no significant difference in seed weight within each group and between groups. Using the method above, adjust the order of soaking for seed germination. The concentration of sodium tungstate is 0.1%, the concentration of hydrogen peroxide is 2%, the concentration of potassium nitrate is 19mmol / L, the concentration of gibberellin is 500mg / L, and the concentration of mercury is 0.1%.

[0056] Among them, the number X is used to represent the Xth soaking, for example: the soaking sequence is: 12345, which means that the first soaking, the second soaking... until the fifth soaking is carried out in sequence; for another example, the soaking sequence is : 13452, indicating that the soaking...

Embodiment 3

[0068] Embodiment three: the concentration of soaking medicinal liquid is preferred

[0069] 1. Select 1,300 seeds of Chonglou yunnanensis that were fresh and mature in the same year, uniform in size, in the same state, and stored for the same storage time, and divided them into 13 groups with 100 seeds in each group. There was no significant difference in seed weight within each group and between groups. Seed germination was performed using the method described above. Wherein, the concentrations of soaking liquids in each group are shown in Table 7.

[0070] Table 7 Concentration of soaking liquid in each group

[0071]

[0072] 2. Calculate the average germination rate of the above-mentioned groups at 30 days, 45 days, 60 days, 90 days and 180 days respectively. Among them, seeds that have not germinated within 180 days are counted as germination failure; 180 days with a germination rate lower than 90% are counted as program failure. The specific results are shown in T...

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Abstract

The invention belongs to the technical field of biology, and particularly relates to a method for promoting rapid germination of paris polyphylla seeds. The method specifically comprises removing seedcoat; soaking with a sodium tungstate solution for 10-14 hours; soaking with hydrogen peroxide for 6-10 hours; soaking with a potassium nitrate solution and a gibberellin solution for 36-48 hours; soaking with hydrogen peroxide for 6-10 hours for the second time; then soaking with the potassium nitrate solution and the gibberellin solution for 36-48 hours for the second time; soaking with alcoholfor 25-30 seconds; continuing to soak with a mercury bichloride solution for 6-10 minutes, washing, drying in a sterile environment, and inoculating to a 1 / 2MS culture medium, wherein the inoculationarea is larger than or equal to 0.09cm<2> / seed. The method has universal applicability to the paris genus seeds; the germination is early, and the germination rate is high, wherein the germination starts on the 30th day, and the average germination rate on the 60th day can be 80% or more. The method fully utilizes the physiological characteristics of the paris polyphylla seeds, and is simple to operate and low in costs of raw materials, and can be popularized to practical application.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a method for promoting the rapid germination of the seeds of the plant. Background technique [0002] The plant of the genus Chonglou is a plant of the Liliaceae family. Its subordinates mainly include Chonglou Yunnan, Aescin, etc. Its rhizomes can be used as medicine. The main raw materials of various Chinese patent medicines and new drugs such as Qingre Zhike Granules and Antiviral Granules. Due to the huge medicinal and economic value of Chonglou, the market has been in short supply for a long time, and the wild resources have been shrinking rapidly. Therefore, the domestic planting model of Chonglou has been born, which mainly includes two methods: asexual reproduction of tubers and sexual reproduction of seedlings. However, the seeds of Chonglou have two characteristics of morphological dormancy and physiological dormancy at the same time. It takes two winters and o...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01C1/00
CPCA01C1/00
Inventor 樊小莉王涛刘琴徐智斌冯波周强杨莉
Owner CHENGDU INST OF BIOLOGY CHINESE ACAD OF S