A cell cryopreservation liquid for human umbilical cord mesenchymal stem cells
A technology of stem cells and human umbilical cord, applied in the field of cell cryopreservation of human umbilical cord mesenchymal stem cells, can solve the problems of pollution, reduce antibiotic content, increase cell survival rate, etc.
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Embodiment 1
[0026] A cell cryopreservation solution for human umbilical cord mesenchymal stem cells, composed of serum-free culture medium, serum substitute, dimethyl sulfoxide, streptomycin sulfate and additives, wherein the percentage of ingredients is: serum-free culture medium: 41.8 %, serum substitute: 50%, dimethylsulfoxide: 8%, 200ug / ml streptomycin sulfate: 0.1%, additive: 0.1%.
[0027] The serum-free medium contains the following components added to the basal culture medium, and the final concentrations of each component are: human serum albumin 1-2 g / L, transferrin 5-10 mg / L, fibronectin 2-2 8mg / L, laminin 1~4mg / L, Fe(NO3)3 9H2O 50g / L, FeSO4 7H2O4 17g / L, estradiol 1~3μg / L, testosterone 2~5μg / L, progesterone 1~3μg / L, dexamethasone 39.25~117.74μg / L, insulin 5~10mg / L, riboflavin 376.36mg / L, coenzyme A80.96~242.87mg / L, butyldiamine 4.41~6.17mg / L, taurine Acid 1~2mg / L, aminoethanol 0.61~1.85mg / L, pyruvic acid 8.81~26.42mg / L, sodium selenate 3.78~7.56μg / L, L-glutamine 292.3~584.6mg...
Embodiment 2
[0038]A cell cryopreservation solution for human umbilical cord mesenchymal stem cells, composed of serum-free culture medium, serum substitute, dimethyl sulfoxide, streptomycin sulfate and additives, wherein the percentage of ingredients is: serum-free culture medium: 46.8 %, serum substitute: 45%, dimethylsulfoxide: 8%, 200ug / ml streptomycin sulfate: 0.1%, additive: 0.1%.
[0039] The serum-free medium contains the following components added to the basal culture medium, and the final concentrations of each component are: human serum albumin 1-2 g / L, transferrin 5-10 mg / L, fibronectin 2-2 8mg / L, laminin 1~4mg / L, Fe(NO3)3 9H2O 50g / L, FeSO4 7H2O4 17g / L, estradiol 1~3μg / L, testosterone 2~5μg / L, progesterone 1~3μg / L, dexamethasone 39.25~117.74μg / L, insulin 5~10mg / L, riboflavin 376.36mg / L, coenzyme A80.96~242.87mg / L, butyldiamine 4.41~6.17mg / L, taurine Acid 1~2mg / L, aminoethanol 0.61~1.85mg / L, pyruvic acid 8.81~26.42mg / L, sodium selenate 3.78~7.56μg / L, L-glutamine 292.3~584.6mg / ...
Embodiment 3
[0050] A cell cryopreservation solution for human umbilical cord mesenchymal stem cells, composed of serum-free culture medium, serum substitute, dimethyl sulfoxide, streptomycin sulfate and additives, wherein the percentage of ingredients is: serum-free culture medium: 51.8 %, serum substitute: 40%, dimethylsulfoxide: 8%, 200ug / ml streptomycin sulfate: 0.1%, additive: 0.1%.
[0051] The serum-free medium contains the following components added to the basal culture medium, and the final concentrations of each component are: human serum albumin 1-2 g / L, transferrin 5-10 mg / L, fibronectin 2-2 8mg / L, laminin 1~4mg / L, Fe(NO3)3 9H2O 50g / L, FeSO4 7H2O4 17g / L, estradiol 1~3μg / L, testosterone 2~5μg / L, progesterone 1~3μg / L, dexamethasone 39.25~117.74μg / L, insulin 5~10mg / L, riboflavin 376.36mg / L, coenzyme A80.96~242.87mg / L, butyldiamine 4.41~6.17mg / L, taurine Acid 1~2mg / L, aminoethanol 0.61~1.85mg / L, pyruvic acid 8.81~26.42mg / L, sodium selenate 3.78~7.56μg / L, L-glutamine 292.3~584.6mg...
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