Application of metformin to reversion of cisplatin resistance of ovarian cancer
A metformin and ovarian cancer technology, applied in the new medical application of metformin, the application field of metformin in reversing cisplatin resistance in ovarian cancer, can solve the problems that plague the treatment of ovarian cancer
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Embodiment 1
[0025] Example 1. Metformin inhibits the proliferation of cisplatin-resistant ovarian cancer cells in vitro
[0026] (1) Detection of the effect of different concentrations of metformin on the proliferation of cisplatin-resistant ovarian cancer cell lines
[0027] Cisplatin-resistant ovarian cancer cell lines CP70 and C13K were divided into 8×10 3 / hole and 1×10 4 The concentration per well was inoculated in a 96-well plate for 24 hours (i.e. after 24 hours of cell starvation, the medium was DMEM / F12, purchased from sigma, catalog number D6241), and replaced with metformin containing different concentrations (purchased from sigma, catalog number No. D150969-5G) complete medium (with no metformin as the control, metformin concentrations were set to 0.01mmol / L, 0.1mmol / L, 1mmol / L and 10mmol / L), after 48 hours with BrdU-ELISA reagent Roche (Cell Proliferation ELISA, BrdU (colorimetric) Cat. No. 11647229001) was used to detect changes in the proliferation of each cell (prolifera...
Embodiment 2
[0032] Example 2. Metformin promotes apoptosis of cisplatin-resistant ovarian cancer cell lines
[0033] Use AnnexinV / PI kit (purchased from Invitrogen Company) to detect by flow cytometry, after obtaining different concentrations of metformin treatment (cultivation method is the same as in Example 1), the results are as follows: figure 2 As shown, it can be seen that the percentage of apoptosis of ovarian cancer drug-resistant cell lines (C13K and CP70) after metformin culture was significantly increased (lower right quadrant), and increased with the increase of metformin concentration.
Embodiment 3
[0034] Example 3. Metformin changes the cell cycle of cisplatin-resistant ovarian cancer cells
[0035] Different concentrations of metformin were used to culture ovarian cancer drug-resistant cell lines (C13K and CP70) for 24 hours (the culture method was the same as in Example 1), and then detected by flow cytometry, the results were as follows: figure 1 As shown, it can be seen that the proportion of cell cycle G1 phase is significantly increased, indicating that cell growth is retarded.
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