Nucleic acid aptamer for recognizing liver cancer cells, and screening method and purpose thereof

Abstract

The invention discloses a nucleic acid aptamer for recognizing liver cancer cells, and a screening method and a purpose thereof. The nucleotide sequence of the nucleic acid aptamer comprises the feature sequence part shown as Seq ID No.1. The purpose comprises the purpose of the nucleic acid aptamer to the design and preparation of anti-liver-cancer medicine or detection reagents. The detection reagents comprise enzyme-linked immunosorbent assay, immunoradiometric assay or fluorescence method. The invention also comprises a target preparation and a medicine composition. The target preparationcomprises the nucleic acid aptamer and a medically acceptable carrier or a shaping agent. The medicine composition comprises the nucleic acid aptamer, at least one antitumor medicine and a medically acceptable carrier. Compared with the prior art, the nucleic acid aptamer can be used for specifically recognizing the liver cancer cells; good application prospects are realized.

Description

technical field [0001] The present invention relates to the field of biomedicine, in particular to a nucleic acid aptamer for recognizing liver cancer cells and its screening method and application. Background technique [0002] Primary liver cancer is one of the most common and most malignant tumors in the world. Therefore, research on the treatment of liver cancer is urgent. In the clinical treatment plan, although surgical treatment can cure liver cancer, it is limited to a small number of patients. For most patients, due to factors such as large lesions or high postoperative recurrence rate, they cannot be cured. Therefore, chemotherapy is still the main treatment method, but the current chemotherapy of liver cancer still lacks effective therapeutic drugs to ensure the chemotherapy effect of liver cancer, and it is urgent to further develop new therapeutic drugs or methods combined with the research on the pathogenesis of liver cancer. [0003] The tumor stem cell hypot...

AI Technical Summary

Problems solved by technology

In the clinical treatment plan, although surgical treatment can cure liver cancer, it is limited to a small number of patients. For most patients, due to factors such as large lesions or high postoperative recurrence rate, they cannot be cured.

Therefore, chemotherapy is still the main treatment method, but the current chemotherapy of liver cancer still lacks effective therapeutic drugs to ensure the chemotherapy effect of liver cancer. It is urgent to further develop new therapeutic drugs or methods in combination with the research on the pathogenesis of liver cancer.

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