Specific sequence of konjac soft rot bacteria, and detection primer and application thereof

Abstract

The invention belongs to the technical field of plant protection and discloses a specific sequence of konjac soft rot bacteria, and a detection primer and application thereof. The nucleotide sequenceof the specific sequence is as shown in SEQ ID NO.1. Compared with identification targets before the application date, the specific detection target of the konjac soft rot bacteria, which is providedby the invention, has the characteristics of good specificity, strong stability and high sensitivity, and related species or subspecies of the konjac soft rot bacteria, which have high homology with the konjac soft rot bacteria can be well distinguished, so that false positive results are avoided. The konjac soft rot bacteria can be detected accurately, and thus a good tool is provided for field detection and early diagnosis of konjac soft rot disease.

Description

technical field [0001] The invention belongs to the technical field of plant protection, and in particular relates to the specific sequence of konjac soft rot pathogen and its detection primer and application. Background technique [0002] Konjac (Amorphophallus konjac) is a perennial herb whose bulbs are rich in glucomannan and widely used in food, chemical engineering, medicine, agriculture, and petroleum (Li et al., 2010). China is the main producer of konjac, with a planting area of ​​about 1.13×10 5 hectares (Wu Jinping, 2014). With the increase of konjac planting and continuous cropping area in my country, the occurrence of konjac bacterial soft rot has increased year by year, resulting in huge losses in konjac production (Wu et al., 2010, 2012). According to statistics, general konjac soft rot can lead to a 30%-50% yield reduction, and in some planting areas, the yield can be reduced by more than 80%, and sometimes even no harvest (Zhang Xin et al., 2010; Wu et al.,...

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Problems solved by technology

[0004] At present, although conventional PCR and real-time quantitative PCR have been applied to the detection of konjac soft rot (Ma Gaizhuan et al., 2009; Wu et al., 2011), but PCR detection technology requires special equipment and high detection cost. It is not suitable for rapid application in the field, and most of the previously reported specific primers for the detection of Konjac soft rot are based on 16S rDNA and UPR specific primers (Ma Gaizhuan et al., 2009; Kang et al., 2003; Wu et al., 2011), but the applicant found that the primers designed for 16SrDNA have high homology bacteria for some closely related species or subspecies, it is difficult to distinguish, especially difficult to distinguish subspecies; and UPR-specific primers expand The added target sequence has high homology with its close relatives, for example, the homology with Pectobacterium carotovorum subsp.odoriferum is as high as 95%, and the homology with Pectobacterium carotovorum is 94%, which will cause Non-specific amplification, causing false positive problems

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