Primer for detecting sulfate reducing bacteria in jet fuel, application and detection method

A detection method and jet fuel technology, which are applied in biochemical equipment and methods, microbial determination/inspection, DNA/RNA fragments, etc. very good effect
CN111363841AInactive Publication Date: 2020-07-03LOGISTICAL ENGINEERING UNIVERSITY OF PLA
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
LOGISTICAL ENGINEERING UNIVERSITY OF PLA
Publication Date
2020-07-03
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention belongs to the technical field of biological detection, and discloses a primer for detecting sulfate reducing bacteria in jet fuel, application and a detection method. The primer comprises outer primers F3 and B3, inner primers FIP and BIP and two loop primers LB and LF; the sequence of the outer primer F3 is shown as SEQ ID NO. 1, the sequence of the outer primer B3 is shown as SEQID NO. 2, the sequence of the inner primer FIP is shown as SEQ ID NO. 3, the sequence of the inner primer BIP is shown as SEQ ID NO. 4, the sequence of the loop primer LB is shown as SEQ ID NO. 5, andthe sequence of the loop primer LF is shown as SEQ ID NO. 6. The method has the advantages of being high in sensitivity and specificity.
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Description

technical field

[0001] The invention relates to the technical field of biological detection, in particular to a primer for detecting sulfate-reducing bacteria in jet fuel, an application and a detection method. Background technique

[0002] The description of the background technology of the present invention belongs to the relevant technology related to the present invention, and is only used to illustrate and facilitate the understanding of the content of the present invention. prior art on the filing date.

[0003] Sulfate-Reducing Bacteria (SRB) is a class of facultative anaerobic microorganisms that can reduce sulfate ions and other oxidized sulfur to sulfide and obtain energy from it. Sulfate-reducing bacteria have tenacious vitality and are widely distributed in nature. They are often found in soil, ocean and oil field pipelines. At the same time, sulfate-reducing bacteria are also the most corrosive and widely distributed microorganisms. There are many kinds of sul...

Claims

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