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60 results about "Desulfobulbus sp." patented technology

Sulfur autotrophic denitrification-based sewage treatment system capable of recycling sulfur

The invention relates to the field of sewage treatment, in particular to a sewage treatment system capable of recycling sulfur based on sulfur autotrophic denitrification, which comprises a pretreatment tank, an aerobic tank, a sulfur autotrophic denitrification reactor, an anaerobic tank and a secondary sedimentation tank which are sequentially connected, and the part of the secondary sedimentation tank containing supernatant mixed liquor is communicated with the aerobic tank; the anaerobic tank contains sulfate reducing bacteria, and sulfate radicals (SO4 < 2->) in the sewage discharged from the sulfur autotrophic denitrification reactor are reduced into S < 2-> by the anaerobic tank under the action of the sulfate reducing bacteria; water containing S < 2-> in the anaerobic tank enters a secondary sedimentation tank, and supernatant mixed liquor in the secondary sedimentation tank flows back to the aerobic tank; sulfur oxidizing bacteria are contained in the aerobic tank, and S < 2-> in the liquid flowing back from the secondary sedimentation tank is oxidized into S < 0 > and S2O3 < 2-> by the aerobic tank under the action of the sulfur oxidizing bacteria. According to the scheme, the problem that more SO4 < 2-> is generated in the current sulfur autotrophic denitrification process is solved, meanwhile, the consumption of electron donors is reduced, and the cost is reduced.
Owner:CHONGQING GELIN ENVIRONMENTAL PROTECTION TECH CO LTD

Water quality monitoring device and monitoring method thereof

The invention discloses a water quality monitoring device and a monitoring method thereof, belongs to the technical field of water quality monitoring, and solves the problems that trace hydrogen sulfide generated by sulfate reducing bacteria in a deposited layer at the bottom of a water tank which is not cleaned for a long time in a low-oxygen environment reacts with a platinum membrane electrode of a residual chlorine sensor to generate a platinum sulfide covering layer; and the electrode reading is continuously virtually high and drifts slowly. Comprising an optical transmitting module, an optical receiving module, a spectrum separation module, a signal conditioning module, a lock-in amplification module, a spectrum feature extraction module and a hydrogen sulfide recognition engine. Residual chlorine is monitored in a non-contact mode, deep ultraviolet pulse light is emitted to penetrate through a water body, a reflection spectrum is received, second derivative spectral characteristics are extracted through multichannel signal processing, and multivariable regression compensation is executed to calculate a real residual chlorine value after hydrogen sulfide interference characteristics are recognized. Meanwhile, the drift trend is quantified based on the double-time-constant integral, graded early warning is triggered, industrial standard signals are finally output, and the problem of electrode contact pollution is avoided in the whole process.
Owner:SHAOXING KEQIAO ENVIRONMENTAL TECH SERVICE CO LTD

Method for treating acid mine wastewater by nanofiber material reinforced sulfate reducing bacteria

The invention discloses a method for treating acid mine wastewater by using nanofiber material reinforced sulfate reducing bacteria, which comprises the following steps: immobilizing the sulfate reducing bacteria by using the nanofiber material as a carrier to obtain immobilized sulfate reducing bacteria, and treating the immobilized sulfate reducing bacteria in the acid mine wastewater. According to the method, the nanofiber material and the sulfate reducing bacteria are combined to efficiently treat the acid mine wastewater, graphene oxide (GO) in the nanofiber material can be reduced into reduced graphene oxide (rGO) by the sulfate reducing bacteria, growth and metabolism of the sulfate reducing bacteria and removal of pollutants in the wastewater are promoted, the maximum Pb < 2 + > adsorption capacity reaches 260.94 mg / g, and the acid mine wastewater can be efficiently treated. The maximum Cr < 6 + > adsorption capacity reaches 130.25 mg / g, and the Pb < 2 + > removal rate of the nanofiber material immobilized sulfate reducing bacteria reaches up to 96%.
Owner:NANJING TECH UNIV

Method for reinforcing denitrification of sulfur circulation microbial system by using siderophore

The invention discloses a method for reinforcing denitrification of a sulfur circulation microbial system by using an iron carrier, and belongs to the technical field of nitrogen-containing wastewater treatment. The method comprises the following steps: inoculating sulfate reducing bacteria and thiobacillus denitrificans into a culture medium containing iron ions or ferrous ions for domestication; and applying the domesticated complex microbial inoculant to wastewater denitrification. In a sulfur circulating microorganism system, Fe < 2 + > can promote the activity of a biological denitrification key enzyme in a biological system by forming an iron carrier in a form of FeS, S < 2-> is fixed in the system and prevented from escaping, and when electron donors in the system are insufficient, the Fe < 2 + > can be used as a substitute electron donor for biological denitrification, so that the requirement on an external carbon source is reduced, and the energy consumption is reduced. And meanwhile, the impact load resistance of the system can be enhanced. Through sulfate reduction and sulfur autotrophic denitrification and iron coupling, sulfate in wastewater is converted into electron donors, a carbon-nitrogen-sulfur-iron biological co-reaction system is formed, the defects of a single-strain denitrification system are overcome, the denitrification effect is enhanced, and energy consumption and cost are reduced.
Owner:XIANGTAN UNIV

Sulfate reducing bacteria with phosphate solubilizing capacity and application thereof in ecological restoration of soil

The invention relates to sulfate reducing bacteria with phosphate solubilizing capacity and application of the sulfate reducing bacteria in soil ecological restoration, and belongs to the technical field of soil environment microorganisms. The sulfate reducing bacterium disclosed by the invention is Desulfosporosinus sp.QY25, and is preserved in the Guangdong Microbial Culture Collection Center on August 6, 2024, and the preservation number of the sulfate reducing bacterium is GDMCC No: 64963. The invention provides a sulfate reducing bacterium Desulfosporosinus sp.QY25 which is high in dissimilatory sulfate reducing capacity and can be used for converting insoluble phosphorus in soil into soluble phosphorus which can be absorbed by plants. The invention further provides a preparation method of the sulfate reducing bacterium Desulfosporosinus sp.QY25 and an application of the sulfate reducing bacterium Desulfosporosinus sp.QY25. The strain can restore soil ecology, neutralize pH, reduce the content of SO4 < 2->, reduce the bioavailability of heavy metals, improve the utilization efficiency of phosphorus in soil, improve the activity of acid phosphatase in tailings and enable plants to grow well.
Owner:SOUTH CHINA NORMAL UNIV

Synthetic microbial agent for treating uranium-contaminated soil and preparation method of synthetic microbial agent

The invention discloses a synthetic microbial agent for treating uranium-contaminated soil and a preparation method of the synthetic microbial agent. The synthetic microbial agent is prepared from the following bacteria: P.adami, P.megatherium, bacillus subtilis, Artobacter volluticus, symbiotic fungi, Aspergillus niger, sulfate reducing bacteria and Shewanella. The symbiotic fungi are mucor lastein, mucor unimingguminum, mucor circinelloides, mucor dense, rhizopus arrhizus and phylum phthochytrium parasitic fungi. The prepared synthetic microbial agent and synthetic microbial embedded balls are applied to uranium-contaminated soil remediation, the key enzyme activity can be effectively improved, the soil metabolism function is enhanced, meanwhile, exchangeable uranium with high ecological risk is converted into stable and low-harm residual uranium, the mobility and bioavailability of uranium in soil are effectively weakened, and the uranium-contaminated soil remediation effect is improved. Therefore, immobilized remediation of uranium is realized, and a good soil uranium fixing effect is achieved.
Owner:SOUTHWEAT UNIV OF SCI & TECH

Bioelectrochemical method for efficiently defluorinating and degrading perfluorinated compounds and application of bioelectrochemical method

The invention relates to a bioelectrochemical method for efficiently removing fluorine and degrading perfluorinated compounds and application of the bioelectrochemical method, and belongs to the technical field of pollutant treatment. According to the method disclosed by the invention, the sulfate reducing flora (SRB) which takes Clostridiumsensuricto as a main dominant bacterium (the relative abundance is 46.06%) is enriched. The SRB rapidly grows on the surface of the cathode by using electrons provided by the cathode, and ferrous sulfide with conductivity is synthesized on the surface of the cathode, so that the resistance is further reduced, and electron transfer between the SRB and the cathode is promoted. The reacted cathode forms a biological membrane with Tissierella (the relative abundance is 41.32%) as a main dominant bacterium, and compared with an electrochemical method without a biological membrane, the bioelectrochemical method has the advantage that the degradation rate and the defluorination rate of perfluorinated compounds are remarkably improved. In addition, by optimizing the composition of the electrolyte, the degradation rate and the defluorination rate can be further improved, and the defluorination rate can reach 90% or above.
Owner:SUN YAT SEN UNIV +1

Method for improving biological sulfate removal efficiency

The invention belongs to the technical field of biological environmental protection, and relates to a method for improving biological sulfate removal efficiency. According to the process, sulfate is better removed through multiple stages, pH adjustment of inlet water is combined with addition of zero-valent iron in the three-stage A bioreactor, FeS precipitation is generated by the zero-valent iron and S < 2-> ions in the system, a large number of S < 2-> ions are removed, meanwhile, the reaction efficiency of sulfate reducing bacteria is promoted, and conversion of sulfate radicals is improved; the dissolved oxygen concentration is controlled in the aerobic tank, sulfur ions are converted, S2-and SO4 < 2-> ions in water are further removed, and the sulfate radical removal rate of final effluent can be larger than or equal to 90%.
Owner:CHAMBROAD CHEM IND RES INST CO LTD

Cyclohexenylsuccinic acid dieshers as bactericidal inhibitors against corrosion

PendingAZ20250215AMicrobiologySuccinic acid
The invention relates to the field of petrochemicals, in particular to the synthesis of diesters of cyclohexenyl succinic acid and their application as bactericidal inhibitors against sulfate-reducing bacteria. General formula: where R=R1= -С6Н11-; -CH2C6H5 R = n-C4H9; R1 = iso-C4H9-; R = n-C4H9; R1 = n-C7H15-; R = n-C4H9; R1 = n-C9H19-; R = С6Н11 -; R1 =-CH2C6H5 diesters of cyclohexenyl succinic acid have been claimed as bactericidal-inhibitors against corrosion.
Owner:ARETN ACADEMICIAN Y H MAMMADALIYEV INSTITUTE OF PETROCHEMICAL PROCESSES

Sulfur conversion driven chlorinated hydrocarbon polluted underground water treatment method

The invention belongs to the technical field of sewage treatment, and particularly relates to a sulfur conversion driven chlorohydrocarbon polluted underground water treatment method. According to the method, the multifunctional biochar is prepared through high-temperature ammonia gas modification, PS can be activated to oxidize and remove chlorinated hydrocarbon, then sulfide reduction is catalyzed to remove residual chlorinated hydrocarbon, a porous structure of BC provides pollutant adsorption and fixation functions, and after MFBC, PS and sulfate reducing bacteria are sequentially added into a chlorinated hydrocarbon pollution medium, the pollution medium is subjected to high-temperature ammonia gas modification to remove the residual chlorinated hydrocarbon. The sulfur-containing species undergo a cyclic conversion process from PS-sulfate ion-sulfide-elemental sulfur-sulfate ion. In the conversion process, various oxidation and reduction active substances can be used for effective detoxification of chlorinated hydrocarbon, and MFBC and sulfate reducing bacteria play a key role in mediating sulfide conversion. According to the method, the chlorohydrocarbon in the high-permeability medium can be rapidly removed firstly, and then the chlorohydrocarbon slowly released by the low-permeability medium can be slowly removed at low cost. And the method is low-carbon and environment-friendly, has no secondary pollution such as heavy metals and has obvious application advantages.
Owner:SOUTHERN UNIVERSITY OF SCIENCE AND TECHNOLOGY

Method for simulating hydrogen permeation value of defective pipeline influenced by corrosion of sulfate reducing bacteria

The invention provides a numerical simulation method for hydrogen permeation of a defective pipeline influenced by corrosion of sulfate reducing bacteria. Comprising the following steps: (1) determining hydrogen permeation key parameters of a pipeline in a sulfate reducing bacteria corrosion environment through a sulfate reducing bacteria corrosion simulation experiment; and (2) constructing a multi-physics field coupling model by utilizing a finite element method, and realizing the numerical simulation of the hydrogen concentration distribution of the defective pipeline under the corrosion influence of the sulfate reducing bacteria through coupling of an electrochemical physics field and a substance transfer physics field. And (3) outputting a hydrogen concentration distribution result of the defect-containing pipeline, and revealing an influence rule of corrosion of the sulfate reducing bacteria on the hydrogen concentration distribution. According to the method, the technical blank of hydrogen permeation numerical simulation of the defect-containing pipeline in a sulfate reducing bacteria corrosion scene is filled, and theoretical support is provided for hydrogen damage evaluation, safety design and protection of the defect-containing pipeline.
Owner:FUZHOU UNIV

Control of sulfate-reducing bacteria under anaerobic conditions using glutaraldehyde and peroxymonosulfate

PCT designated stageWO2025240302A1BiocideDisinfectantsBiotechnologyMicroorganism
Disclosed herein are methods for controlling anaerobic microorganisms, especially sulfate -reducing bacteria, in anaerobic environments or under anaerobic conditions in water-containing systems using a combination of glutaraldehyde and an alkali metal or alkaline earth metal peroxymonosulfate.
Owner:LANXESS CORPORATION

Sulfate-reducing bacteria culture medium and application thereof

The application provides a sulfate-reducing bacteria culture medium and application thereof, and components of the culture medium include: a carbon source, a nitrogen source, a phosphorus source, calcium chloride, magnesium chloride, an inorganic sulfur source, an organic sulfur compound with reducibility, inorganic trace elements, agar and water; wherein the mass content of the organic sulfur compound with reducibility is 5-30 mg / L, the mass content of the inorganic trace elements is 152-365 mg / L; the organic sulfur compound with reducibility includes lipoic acid and / or thiamine hydrochloride; and the inorganic trace elements include a combination of zinc elements, manganese elements, molybdenum elements and boron elements. The application introduces inorganic trace elements required by biological cell metabolism and rich sulfur electron acceptor species, promotes the growth and metabolism process of sulfate-reducing bacteria, and enables SRB cells to form visible colonies at a faster speed.
Owner:PETROCHINA CO LTD

SRB biological reaction network and preparation method and application thereof

The present application relates to the technical field of heavy metal pollution remediation, and particularly relates to a SRB biological reaction net and a preparation method and application thereof.The present application provides a preparation method of the SRB biological reaction net, comprising the following steps: (1) mixing and stirring iron manganese phosphate, sulfate-reducing bacteria and a sodium alginate solution to obtain a mixed solution; (2) immersing a metal net in the mixed solution, and then taking out the metal net and placing it in a calcium chloride solution for solidification.The SRB biological reaction net prepared by the present application can improve the activity of sulfate-reducing bacteria, weaken the influence of heavy metals on the sulfate-reducing bacteria, reduce the loss of bacterial bodies and materials caused by groundwater flow, and improve the material utilization rate.The preparation method of the present application is simple, the product has good stability, and can be widely used.
Owner:BEIJING RESEARCH INSTITUTE OF CHEMICAL ENGINEERING AND METALLURGY +1

A method for controlling sulfate-reducing bacteria using a bacteriophage

The application discloses a method for controlling sulfate-reducing bacteria by using bacteriophages. The application provides a method for inhibiting hydrogen sulfide production in oilfields, which comprises the following steps: 1) filtering oilfield produced liquid and collecting filtrate; the filtering is performed by using a filter membrane with a pore size of 0.2 microns, or by using a filter membrane with a pore size of 0.2 microns in combination with a filter membrane with a pore size of 0.1 micron; 2) concentrating the filtrate to obtain concentrated filtrate, so that the concentration of bacteriophages in the concentrated filtrate is greater than or equal to 1.0*10 6 / ml; and 3) injecting the concentrated filtrate collected in the step 2 back into the oilfield to realize the inhibition of hydrogen sulfide production in the oilfield. The experiment of the application proves that the bacteriophage concentrate can inhibit the production of sulfide and hydrogen sulfide by sulfate-reducing bacteria; microorganisms with a size greater than 0.1 micron and less than 0.2 micron can promote the bacteriophage concentrate to inhibit the production of sulfide and hydrogen sulfide by sulfate-reducing bacteria.
Owner:WEILI BIOTECHNOLOGY (WEIHAI) CO LTD

Rapid analysis method of metal corrosive microorganism sulfate reducing bacteria

The invention provides a rapid analysis method of metal corrosive microorganism sulfate reducing bacteria, and belongs to the technical field of biology.The rapid analysis method comprises the steps that a to-be-detected microorganism genome DNA sample and a photosensitizer As2MoO6 atNiS are taken to jointly construct a triple asymmetric photon PCR system, asymmetric photon PCR amplification is carried out under the condition of LED light source excitation, after amplification is completed, the sample is detected, and then the sample is detected. The obtained amplification product is mixed with a photo-thermal signal probe prepared from a photosensitizer As2MoO6 coated NiS, and photo-thermal temperature change detection is carried out. The rapid analysis method provided by the invention has the characteristics of high efficiency, convenience and accuracy, and is suitable for rapid detection of sulfate reducing bacteria.
Owner:ZHEJIANG UNIV CITY COLLEGE

Fluorescent probe, fluorescent probe kit and method for detecting sulfate-reducing bacteria

The present invention provides a fluorescent probe, a fluorescent probe kit and a method for detecting sulfate-reducing bacteria. The fluorescent probe has the following structural formula. Divalent sulfur anions are used to replace fluorescent molecules in the fluorescent probe, thereby separating metal ions from the fluorescent molecules, and the fluorescent molecules re-exhibit fluorescent properties. Moreover, experiments have shown that when hydrogen sulfide is further used to provide divalent sulfur anions for nucleophilic substitution with the fluorescent probe, the maximum fluorescence intensity of the fluorescent molecule is linearly related to the concentration of hydrogen sulfide, and the concentration of hydrogen sulfide can be characterized. The above-mentioned existing detection methods have proved that the concentration of hydrogen sulfide and the bacterial content of sulfate-reducing bacteria are linearly related, and the above-mentioned fluorescent molecules can be used to make fluorescent probes to detect the bacterial content of sulfate-reducing bacteria. The method for detecting sulfate-reducing bacteria based on the above-mentioned fluorescent probe does not require the expansion and cultivation of sulfate-reducing bacteria, can be used to detect the substance to be detected, and is easy to operate.
Owner:CHINA SPECIAL EQUIP INSPECTION & RES INST +1

Bio-based oxygen and acid inhibitor as well as preparation method and application thereof

The invention discloses a bio-based oxygen and acid inhibitor and a preparation method and application thereof, and belongs to the technical field of mine acid wastewater source control, and the preparation method comprises the following steps: mixing sulfate reducing bacteria sludge and municipal sewage activated sludge according to a mass ratio of 1: (2-5), and carrying out hydrolysis reaction at 150-200 DEG C; sodium carbonate and calcium chloride are mixed according to the mass ratio of (1.2-1.5): 1, then the mixture is added into the mixed sludge obtained after the hydrolysis reaction, and the bio-based oxygen and acid inhibitor is obtained. The bio-humus contained in the bio-based oxygen barrier inhibitor and metal ions contained in ore generate a cross-linking reaction, sulfate reducing bacteria release biomass to induce biological inhibition on iron-sulfur oxidizing bacteria, the dual effects of synchronous oxygen barrier and biological inhibition are achieved, generation of acid mine wastewater is inhibited from multiple angles, and the biological inhibition effect is good. And the bio-based oxygen-blocking bacteriostatic agent is biodegradable, environment-friendly in material selection, wide in raw material source and convenient to operate, and has a relatively good application prospect in the aspect of acidic wastewater source control in mineral separation and processing industries.
Owner:GUANGDONG GUANGYE YUNLIU MINING CO LTD +1

Device and method for carrying out end treatment on old kiln water by using corn straws

The invention discloses a device and a method for carrying out end treatment on old kiln water by using corn straws, and belongs to the technical field of water treatment.The method comprises the following steps: carrying out vulcanization precipitation and neutralization precipitation on the old kiln water, and then refluxing a sulfate ion aqueous solution without heavy metal ions into a two-phase reactor B, the toxic action of heavy metal ions on sulfate reducing bacteria is effectively weakened. According to the method, two processes of hydrolysis and pyrolysis are simultaneously adopted for the corn straws, so that small-molecular organic matters such as acetic acid, lactic acid and ethanol which are required by sulfate reducing bacteria are obtained as electron donors, and the biochar which can be used for advanced treatment of water quality is also obtained. A bioreactor of sulfate reducing bacteria is divided into a two-phase reactor A and a two-phase reactor B, and the reaction environments of the two-phase reactor A and the two-phase reactor B are controlled through purified water reflux and sulfate radical reflux, so that the competitive effect of anaerobic microorganisms is effectively solved.
Owner:河北省煤田地质局新能源地质队

Method for heavy metal removal in oligotrophic environments using semiconductor mineral-strengthened microorganisms

The application discloses a method for removing heavy metal in oligotrophic environment by using semiconductor mineral reinforced microorganisms, which utilizes light energy to replace organic matter to drive sulfate-reducing bacteria metabolism to remove heavy metal ions. The sphalerite produced by microbial metabolism or natural sphalerite or chemically synthesized sphalerite is excited to produce photoelectrons under light conditions, and the sulfate-reducing bacteria with extracellular electron transfer capacity utilize the photoelectrons to carry out sulfate reduction metabolism to produce hydrogen sulfide, which reacts with heavy metal ions (such as lead ions) in wastewater to form heavy metal sulfide precipitates such as lead sulfide, so as to remove the heavy metal ions from the water phase. The method cooperatively utilizes light energy and chemical energy to efficiently treat heavy metal pollution, and simultaneously avoids problems such as secondary pollution, active sludge production and greenhouse gas emission in the treatment process, has low treatment cost, is environment-friendly, and has a wide application prospect.
Owner:PEKING UNIV

Organic complexes of hexamethylenediamine amide of oleic acid as bactericidal inhibitors

PendingAZ20250276APolymer scienceHexamethyldiamine
The invention relates to the field of petrochemicals, in particular to the preparation of organic complexes of hexamethylenediamine amide of oleic acid and their use as bactericidal-inhibitors against sulfate-reducing bacteria. The organic complexes of hexamethylenediamine amide of oleic acid, having the general formula: [C17H33CONH(CH2)6NH3] + [COOR] – where R=H or R=CH3, are claimed as bactericidal-inhibitors.
Owner:ARETN ACADEMICIAN Y H MAMMADALIYEV INSTITUTE OF PETROCHEMICAL PROCESSES

Method for realizing long-acting control of sulfate reducing bacteria by inhibiting hydrogen metabolism and application

The invention discloses a method for realizing long-acting control of sulfate reducing bacteria by inhibiting hydrogen metabolism and application, and belongs to the technical field of microorganism prevention and control. According to the method, polysulfide is added into a liquid microenvironment where sulfate reducing bacteria survive, and the method is suitable for the liquid environment where biological acidification / corrosion inhibitors such as sulfate analogues and nitrate exist and can be applied to biological acidification / biological corrosion control in the environments of oil reservoirs, produced water and the like. The method provided by the invention overcomes the defects of existing technologies of controlling biological acidification by adding nitrate and sulfate analogues and the like, and realizes effective inhibition of metabolic activity and species abundance of sulfate reducing bacteria in environments with high organic matter electron donors and low sulfate electron acceptors; and the progress of the oil reservoir biological acidification and biological corrosion control technology is promoted in principle.
Owner:PEKING UNIV

Method for preparing sulfate reducing bacteria (SRB) microbial inoculum by using active carbon (HC)

The invention discloses a method for preparing a sulfate reducing bacteria (SRB) microbial inoculum by using activated carbon (HC). According to the invention, the HC is used as a cheap fixing carrier for fixing the SRB, so that the rate and the acid resistance of the SRB for treating the acid mine wastewater (AMD) can be effectively improved. The method provided by the invention has the advantages of high treatment efficiency and low cost, can relieve acid stress when the AMD is treated by SRB, and is an immobilization method which is high in efficiency, easy to operate and free of pollution.
Owner:TIANJIN UNIV +1

A method of preventing leakage in a carbon dioxide geological storage process

The application provides a method for preventing leakage in a carbon dioxide geological storage process, comprising the following steps: preparing a sulfate-reducing bacteria culture medium; extracting sulfate-reducing bacteria, culturing the sulfate-reducing bacteria in the sulfate-reducing bacteria culture medium at 28-38 DEG C to obtain cultured sulfate-reducing bacteria; for a stratum region with a sulfate mineral content of no less than 5 wt.%, exploring the stratum, selecting a stratum with a high sulfate mineral content, injecting the cultured sulfate-reducing bacteria into fractures in the stratum through a filling pipe, and standing; for a cap rock region with a sulfate mineral content of less than 5 wt.%, mixing sulfate and the cultured sulfate-reducing bacteria, standing to obtain a mixed solution, and then injecting the mixed solution into fractures in the stratum, and standing. The technical scheme of the application forms a cemented filling material through the interaction between microorganisms and stratum minerals, and can effectively seal fractures around an injection well in a micro view.
Owner:SHENZHEN POLYTECHNIC

Construction of a method for detecting sulfate-reducing bacteria metabolic enzyme function genes

The present application relates to a kind of functional gene detection method, specifically sulfate-reducing bacteria metabolic enzyme functional gene detection method.The specific sequence of three different hairpin DNA chains is designed according to the specificity of sulfate-reducing bacteria metabolic enzyme, after mixing three different hairpin DNA chains, incubation is carried out by adding the sample to be detected, after incubation, hemin, TMB and H2O2 are added to the system to react, and the key metabolic enzyme DNA of sulfate-reducing bacteria in the sample to be measured is qualitatively / quantitatively detected by color reaction.The preparation method of the present application is simple to operate, and the reaction is rapid, and visible to naked eye.The construction of sulfate-reducing bacteria metabolic enzyme functional gene detection method has potential application value in the research fields of molecular biology, biological analytical chemistry and medical detection.
Owner:INST OF OCEANOLOGY - CHINESE ACAD OF SCI

Rapid detection device for sulfate reducing bacteria

The utility model relates to the technical field of sulfate reducing bacteria detection, in particular to a rapid detection device for sulfate reducing bacteria. The rapid detection device for the sulfate reducing bacteria comprises a detection bottle, a detachable bottle cap is mounted on the detection bottle, a mounting through cavity is formed in the bottle cap, an elastically connected storage barrel is inserted into the mounting through cavity, a dipping carbon column is stored in the storage barrel, and an elastic telescopic sleeve is mounted on the outer barrel wall of the storage barrel. According to the rapid detection device for the sulfate reducing bacteria, the storage barrel is pressed downwards to drive the storage barrel to slide into the detection bottle, at the moment, the spring is compressed, after the storage barrel slides to a proper height, the fastening screw is rotated to enable the storage barrel to be fixed on the guide rod, at the moment, the elastic telescopic sleeve is contracted, and the storage barrel is not prone to falling off due to the through groove formed in the periphery. Therefore, unreacted H2S can be quickly absorbed by the impregnated carbon column in the storage cylinder, and the hidden danger that H2S overflows after the detection bottle is opened is avoided.
Owner:XINJIANG SHENGLI XINGKE TESTING TECH CO LTD

Method for improving stress resistance of plants

The invention discloses a method for improving stress resistance of plants, and belongs to the technical field of microorganisms for agriculture and forestry. The invention discloses a microbial agent for improving waterlogging tolerance of plants. The microbial agent is prepared from the following components in parts by weight: 20-30 parts of denitrifying bacteria, 15-20 parts of sulfate reducing bacteria, 7-10 parts of special methanogenic bacteria and 3-5 parts of candida utilis. The microbial agent provided by the invention can relieve adverse effects of waterlogging stress on normal growth and development of plants, can significantly improve waterlogging tolerance of the plants and increase the survival rate of the plants, and has wide application value in agriculture and forestry production.
Owner:GUANGXI FORESTRY RES INST

A method for characterizing the particle size and composition of nanoparticles using asymmetric flow-field coupled inductively coupled plasma atomic emission spectrometry.

This invention provides a method for characterizing the particle size and composition of nanoparticles using asymmetric flow-field coupled inductively coupled plasma atomic emission spectrometry (ICP-AES), comprising: S1. pre-treating the asymmetric flow-field instrument in an anaerobic environment; S2. loading a sulfate-reducing bacteria-treated sulfate solution into the asymmetric flow-field instrument, and obtaining the characterization results after a sample introduction stage, a flushing stage, and a rinsing stage. This invention utilizes sulfate-reducing bacteria in conjunction with an asymmetric flow-field instrument to detect sulfate solutions, using the sulfate-reducing bacteria to convert sulfate ions into sulfur (S), thereby achieving effective detection of sulfate ions.
Owner:SUN YAT SEN UNIV

Baffled membrane bioreactor and method for partition recycling of sulfate wastewater

The invention provides a baffled membrane bioreactor and a method for partition recycling of sulfate wastewater, and relates to the field of wastewater treatment. The reactor is divided into a sulfate reduction compartment, an anaerobic-micro-aerobic transition compartment and a sulfur oxidation compartment which are sequentially connected in series by baffle plates arranged in the vertical direction, wherein a first filler area is arranged in the sulfate reduction compartment; a gas curtain device and a second filler area are sequentially arranged in the anaerobic-micro-aerobic transition compartment from bottom to top; and a third filler area and a sulfur trapping device are sequentially arranged in the sulfur oxidation compartment from bottom to top. The first filler area, the second filler area and the third filler area are respectively used for filling a filler loaded with sulfate reducing bacteria, a H2S adsorption filler and a filler loaded with sulfur oxidizing bacteria. According to the invention, sulfate reducing bacteria and sulfate oxidizing bacteria are effectively separated in space, and the problems of poor system stability, low sulfur conversion rate and low recovery rate caused by cross interference and flora inhibition in a reaction zone are solved.
Owner:BEIJING UNIV OF CIVIL ENG & ARCHITECTURE +1

Preparation method of broad-spectrum sterilization and desulfurization integrated agent for oil field water treatment

The invention discloses a broad-spectrum sterilization and desulfurization integrated agent for oil field water treatment, which is characterized by comprising the following components in parts by weight: 20-30 parts of hydroxyl triazine derivative, 10-15 parts of quaternary ammonium salt bactericide, 5-10 parts of organic guanidine, 5-10 parts of surfactant, 10-20 parts of solvent and 30-40 parts of distilled water. The triazine derivative is an excellent desulfurizing agent, stable desulfurization reaction is guaranteed, the reaction process is rapid, and the selectivity is high; the trifluoromethylaniline is connected to triazine derivative molecules, so that the medicament can be effectively adsorbed on the surface of sulfate reducing bacteria, the capability of exchanging ions with the outside is reduced, the growth of the bacteria is inhibited, the bacteria are killed, and the sterilization effect is improved without influencing the sulfur removal effect.
Owner:扬州润达油田化学剂有限公司