15 results about "Desulfobulbus sp." patented technology

The invention relates to the field of petrochemicals, in particular to the synthesis of diesters of cyclohexenyl succinic acid and their application as bactericidal inhibitors against sulfate-reducing bacteria. General formula: where R=R1= -С6Н11-; -CH2C6H5 R = n-C4H9; R1 = iso-C4H9-; R = n-C4H9; R1 = n-C7H15-; R = n-C4H9; R1 = n-C9H19-; R = С6Н11 -; R1 =-CH2C6H5 diesters of cyclohexenyl succinic acid have been claimed as bactericidal-inhibitors against corrosion.

The invention provides a numerical simulation method for hydrogen permeation of a defective pipeline influenced by corrosion of sulfate reducing bacteria. Comprising the following steps: (1) determining hydrogen permeation key parameters of a pipeline in a sulfate reducing bacteria corrosion environment through a sulfate reducing bacteria corrosion simulation experiment; and (2) constructing a multi-physics field coupling model by utilizing a finite element method, and realizing the numerical simulation of the hydrogen concentration distribution of the defective pipeline under the corrosion influence of the sulfate reducing bacteria through coupling of an electrochemical physics field and a substance transfer physics field. And (3) outputting a hydrogen concentration distribution result of the defect-containing pipeline, and revealing an influence rule of corrosion of the sulfate reducing bacteria on the hydrogen concentration distribution. According to the method, the technical blank of hydrogen permeation numerical simulation of the defect-containing pipeline in a sulfate reducing bacteria corrosion scene is filled, and theoretical support is provided for hydrogen damage evaluation, safety design and protection of the defect-containing pipeline.

The application provides a sulfate-reducing bacteria culture medium and application thereof, and components of the culture medium include: a carbon source, a nitrogen source, a phosphorus source, calcium chloride, magnesium chloride, an inorganic sulfur source, an organic sulfur compound with reducibility, inorganic trace elements, agar and water; wherein the mass content of the organic sulfur compound with reducibility is 5-30 mg / L, the mass content of the inorganic trace elements is 152-365 mg / L; the organic sulfur compound with reducibility includes lipoic acid and / or thiamine hydrochloride; and the inorganic trace elements include a combination of zinc elements, manganese elements, molybdenum elements and boron elements. The application introduces inorganic trace elements required by biological cell metabolism and rich sulfur electron acceptor species, promotes the growth and metabolism process of sulfate-reducing bacteria, and enables SRB cells to form visible colonies at a faster speed.

The present application relates to the technical field of heavy metal pollution remediation, and particularly relates to a SRB biological reaction net and a preparation method and application thereof.The present application provides a preparation method of the SRB biological reaction net, comprising the following steps: (1) mixing and stirring iron manganese phosphate, sulfate-reducing bacteria and a sodium alginate solution to obtain a mixed solution; (2) immersing a metal net in the mixed solution, and then taking out the metal net and placing it in a calcium chloride solution for solidification.The SRB biological reaction net prepared by the present application can improve the activity of sulfate-reducing bacteria, weaken the influence of heavy metals on the sulfate-reducing bacteria, reduce the loss of bacterial bodies and materials caused by groundwater flow, and improve the material utilization rate.The preparation method of the present application is simple, the product has good stability, and can be widely used.

The application discloses a method for controlling sulfate-reducing bacteria by using bacteriophages. The application provides a method for inhibiting hydrogen sulfide production in oilfields, which comprises the following steps: 1) filtering oilfield produced liquid and collecting filtrate; the filtering is performed by using a filter membrane with a pore size of 0.2 microns, or by using a filter membrane with a pore size of 0.2 microns in combination with a filter membrane with a pore size of 0.1 micron; 2) concentrating the filtrate to obtain concentrated filtrate, so that the concentration of bacteriophages in the concentrated filtrate is greater than or equal to 1.0*10 6 / ml; and 3) injecting the concentrated filtrate collected in the step 2 back into the oilfield to realize the inhibition of hydrogen sulfide production in the oilfield. The experiment of the application proves that the bacteriophage concentrate can inhibit the production of sulfide and hydrogen sulfide by sulfate-reducing bacteria; microorganisms with a size greater than 0.1 micron and less than 0.2 micron can promote the bacteriophage concentrate to inhibit the production of sulfide and hydrogen sulfide by sulfate-reducing bacteria.

The invention relates to the field of petrochemicals, in particular to the preparation of organic complexes of hexamethylenediamine amide of oleic acid and their use as bactericidal-inhibitors against sulfate-reducing bacteria. The organic complexes of hexamethylenediamine amide of oleic acid, having the general formula: [C17H33CONH(CH2)6NH3] + [COOR] – where R=H or R=CH3, are claimed as bactericidal-inhibitors.

The application provides a system and method for treating high-concentration organic wastewater containing sulfate, belonging to the technical field of industrial wastewater treatment, and is used for solving the problems that in the related art, sulfate-reducing bacteria and methanogens compete for substrates, resulting in low methanogenesis efficiency, and sulfide accumulation inhibits microbial activity. The system separates and couples a methanogenesis electrochemical oxidation unit and a sulfate bioreduction and sulfur fixation unit through an ion exchange membrane and an external circuit, constructs a cross-serial multi-stage treatment structure, integrates intelligent collaborative control and sludge resourceization units, realizes the simultaneous performance of efficient methanogenesis of organic matter, deep sulfate reduction and in-situ sulfur fixation, has high removal rates of COD and sulfate in the treated effluent, has low H2S content in the produced biogas, and can recover sulfur and iron resources.

The invention relates to the technical field of mine environment pollution prevention and control, in particular to a method for cooperatively treating iron in mine wastewater by using iron oxidizing bacteria and sulfate reducing bacteria. According to the method, soluble iron in the AMD is removed by utilizing the microorganisms with the iron oxidation function, sulfate radicals in the AMD are removed by utilizing the microorganisms with the sulfate reduction function, and on the basis, hydrogen sulfide generated in the sulfate reduction process is used for reducing residual ferric iron in the AMD subjected to iron-oxidizing bacterium mineralization treatment; therefore, the iron oxidation and mineralization circulation of the microorganisms is established.

The invention discloses application of a bioelectricity-Fenton system driven by sulfate reducing strain shewanella in environmental pollutant degradation, and belongs to the technical field of bioelectricity-Fenton. In the constructed biological electro-Fenton system, hydrogen sulfide generated by reducing sulfate by the sulfate reducing strain shewanella participates in the biological electro-Fenton system to realize reduction of Fe < 3 + >, and the generated Fe < 2 + > reacts with hydrogen peroxide generated by electrolysis to synchronously generate hydroxyl free radicals. OH, so that efficient degradation of environmental pollutants is realized. According to the constructed bio-electro-Fenton system, sulfate ions commonly existing in the environment can be efficiently utilized, synchronously generated hydrogen sulfide participates in the Fenton system, pollutants in the environment can be efficiently removed through generated hydroxyl free radicals, pollution is avoided, the degradation cost is low, and the efficiency is high.

The present application relates to the field of sewage treatment, and particularly relates to a sewage treatment system capable of sulfur resource utilization based on sulfur autotrophic denitrification, which comprises a pretreatment tank, an aerobic tank, a sulfur autotrophic denitrification reactor, an anaerobic tank and a secondary sedimentation tank connected in sequence, and the part containing supernatant mixed liquid of the secondary sedimentation tank is communicated with the aerobic tank; the anaerobic tank contains sulfate-reducing bacteria, and the sulfate (SO4 2‑ ) in the sewage discharged from the sulfur autotrophic denitrification reactor is reduced to S 2‑ by the sulfate-reducing bacteria in the anaerobic tank; the water containing S 2‑ in the anaerobic tank enters the secondary sedimentation tank, and the supernatant mixed liquid in the secondary sedimentation tank returns to the aerobic tank; the aerobic tank contains sulfur-oxidizing bacteria, and S 2‑ in the liquid returned from the secondary sedimentation tank is oxidized to SO and S2O3 2‑ by the sulfur-oxidizing bacteria in the aerobic tank. Through the present application, the problem that more SO4 2‑ is produced in the current sulfur autotrophic denitrification process is solved, and the consumption of the electron donor is reduced, thereby reducing the cost.

The invention discloses a microbial remediation method for metal sulfide mine waste ore. The microbial remediation method comprises the following steps: S1, screening indigenous sulfate reducing bacteria; s2, preparing a passivation material; s3, after the surface of the waste ore is flattened, a passivation material is laid; and S4, herbaceous plants and shrub plants are planted on the passivated material, and regular inspection is conducted till repairing is completed. According to the method, a microorganism and vegetation metal sulfide waste rock self-repairing system can be formed, acid production and heavy metal production of the waste rock can be inhibited, and long-acting stability of passivation of the waste rock in the metal sulfide mining area and ecological restoration is achieved.

The application discloses a preparation method and use method of sulfate-reducing bacteria modified nano zero-valent iron, and particularly relates to the field of water environment pollution remediation, and comprises the preparation method of sulfate-reducing bacteria modified nano zero-valent iron, a sulfate-reducing bacteria modified nano zero-valent iron and a method for removing sulfamethoxazole (SMX) in water by the sulfate-reducing bacteria modified nano zero-valent iron. The preparation method of the sulfate-reducing bacteria modified nano zero-valent iron is more green and environmentally friendly than the physical and chemical sulfur modification nZVI preparation method, has no energy, ball milling waste or chemical reagent waste residue, and the process raw material source is stable; the prepared sulfate-reducing bacteria modified nano zero-valent iron material (SRB-nZVI) can more efficiently and quickly remove SMX than nZVI or SRB bacterial solution alone; compared with nZVI alone, the SRB-nZVI material can obviously improve the removal rate and removal rate within 7h for removing SMX, the removal rate within 7h can reach 75%, and the final removal rate can reach more than 93%.

The invention belongs to the technical field of acid wastewater treatment, and particularly relates to a sulfate reducing bacteria nutritional agent as well as a preparation method and application thereof. According to the invention, fermentation liquor of non-edible plants which is rich in amino acids (greater than or equal to 20 g / L) and has soluble chemical oxygen demand greater than or equal to 2200 mg / L is used as a basic carrier, exogenous nutrient substances and electron mediators are compounded, and the fermentation liquor and the nutrient substances of the non-edible plants provide efficient electron donors and nutritional ingredients for sulfate reducing bacteria; the electron mediator improves the electron transfer efficiency of the SRB in an anoxic environment. According to the method, the acidic mine wastewater and the sulfate reducing bacteria are subjected to adaptive culture, so that the SRB adapts to the acidic and high-sulfate environment of AMD, the metabolic efficiency of the sulfate reducing bacteria can be remarkably improved through the synergistic effect of the two modes, the growth rate of the sulfate reducing bacteria and the sulfate reduction rate are increased, and the method has the advantages of low cost, high recycling degree, easiness in large-scale production and the like.

The application relates to the technical field of water treatment, and discloses a method for recovering high-purity sulfur from wastewater based on electrochemical coupling biology, which comprises the following steps: 1) hydrogen autotrophic sulfate-reducing bacteria are used to reduce SO4 2‑ in wastewater into HS ‑ in a cathode of an electrolytic hydrogen production reactor; 2) CO2 is introduced as a carbon source, HS ‑ in cathode liquid is blown off in the form of H2S into a Fe(CN)6 3‑ / Fe(CN)6 4‑ delocalized electrochemical oxidation unit of a redox mediator, H2S is selectively oxidized into S 3‑ in the unit, the generated Fe(CN)6 0 is collected, and the generated Fe(CN)6 4‑ is introduced into an anode to be oxidized into Fe(CN)6 3‑ . The application realizes efficient removal of SO4 2‑ in wastewater and synchronous recovery of high-purity S 0 under the condition of low-carbon mode driven by electricity, and solves the problems of low S 0 purity, excessive oxidation and electrode passivation in traditional bioelectrochemical methods.

The invention relates to the field of petrochemicals, in particular to the synthesis of monoesters of cyclohexenyl succinic acid and their application as bactericides-inhibitors against sulfate-reducing bacteria. General formula: Monoesters of cyclohexenyl succinic acid, where R = C4H9-; C9H19-; -CH2C6H5, have been claimed as bactericidal corrosion inhibitors.