31results about How to "Very good" patented technology

The invention discloses a method for separating and purifying pyrroloquinoline quinone in fermentation liquid by a molecularly imprinted solid-phase extraction method. According to the method, the pyrroloquinoline quinone is obtained by performing separation and purification on the fermentation liquid rich in the pyrroloquinoline quinone through a pyrroloquinoline quinone molecularly imprinted polymer. According to the method, silicon dioxide is used as a base material, the pyrroloquinoline quinone is used as a template molecule, methacrylic acid is used as a functional monomer, ethylene glycol dimethacrylate is used as a cross-linking agent, and the pyrroloquinoline quinone molecularly imprinted polymer is obtained through a thermal polymerization technique. Adsorptivity experiments prove that the pyrroloquinoline quinone molecularly imprinted polymer prepared by the method disclosed by the invention has good specific selective properties of adsorbing target objects namely the pyrroloquinoline quinone, and the largest adsorption volume can reach 10mg / mg (molecularly imprinted polymers). Solid-phase extraction is performed on the target object namely the pyrroloquinoline quinone by using the polymer as a solid-phase extraction filling, so that the purpose of efficiently separating and purifying the pyrroloquinoline quinone from the fermentation liquid is realized.

The invention belongs to the technical field of plant protection, and discloses the specific sequence of blackleg pathogen of rapeseed, LAMP detection primers and applications. The invention provides a specific detection target of L.biglobosa, and the target has good specificity, strong stability and high sensitivity. characteristics, it can distinguish the closely related species of Brassica napus with higher homology, avoiding the generation of false positives. The LAMP primers designed for this target can detect 112 fg / μL of L.biglobosa genomic DNA, which provides a good tool for field detection of Brassica napus and rapid high-throughput inspection and quarantine at ports.

The present invention discloses the composition and producing process of rubber for brake. The rubber consists of synthetic rubber, sulfurizing agent, antiageing agent, activator, tackifier, stearic acid, promoter, reinforcing agent, platicizer, etc. The production process includes raw rubber plastication, mixing, hotmixing, forming and sulfurizing, and can obtain optimal material combination. The rubber product has raised stability against heat, oxygen and ageing, heat resistance, airtight performance, etc.

The invention discloses a gamma delta T cell surface-active molecule and a kit for quickly diagnosing active tuberculosis. According to the gamma delta T cell surface-active molecule and the kit disclosed by the invention, a specific T cell surface molecule is applied to the diagnosis of active tuberculosis for the first time; the gamma delta T cell surface-active molecule comprises a myeloid cell induced receptor 1 and / or a V delta 2T cell receptor; by using the two cell surface molecules and corresponding kits thereof, quick detection of active tuberculosis can be realized; the detection method and the detection kit of active tuberculosis have the advantages of high sensitivity, good specificity, simple operation, simplicity, convenience, quickness and the like; meanwhile, the detection method and the detection kit are low in price and are suitable for popularization and application.

The invention belongs to the technical field of plant protection, and discloses the specific sequence of konjac soft rot pathogen and its detection primer and application. The nucleotide sequence of the specific sequence is shown in SEQ ID NO.1. The konjac soft rot provided by the invention Compared with the identification target before the application date, the specific detection target of putrefaction fungus has the characteristics of good specificity, strong stability and high sensitivity. Very good distinction, avoiding the generation of false positives. The existence of konjac soft rot can be accurately detected, which provides a good tool for field detection of konjac soft rot and early diagnosis of the disease.

The invention discloses a reagent set and a method for detecting avian influenza viruses and chicken parvoviruses. The reagent set for detecting or assisting in detecting the avian influenza viruses and the chicken parvoviruses comprises a primer pair named as M-P and a primer pair named as ChPV-P, the M-P comprises two single-chain DNA (deoxyribonucleic acid) shown as SEQ ID No.1 and SEQ ID No.2 in a sequence table, and the ChPV-P comprises two single-chain DNA shown as SEQ ID No.3 and SEQ ID No.4 in the sequence table. Experimental results show that the reagent set and the method for detecting or assisting in detecting the avian influenza viruses and the chicken parvoviruses are good in specificity and accuracy and high in sensibility and can be used for specifically detecting the avian influenza viruses and the chicken parvoviruses, and the sensibility of detecting the avian influenza viruses and the chicken parvoviruses is 100fg.

The invention provides a general foot-and-mouth disease virus structural protein antibody and a blocking ELISA detection reagent kit thereof, and belongs to the technical field of virus detection. Thegeneral foot-and-mouth disease virus structural protein antibody and the blocking ELISA detection reagent kit thereof comprise the following composition of an antigen coating elisa plate and a biotinmarker concentrating monoclonal antibody, wherein a monoclonal antibody in the biotin marker concentrating monoclonal antibody is a monoclonal antibody E32, and the antigen coating elisa plate is anFMDV antigen indirectly coated through a monoclonal antibody F104. The E32 antibody and the F104 antibody are specifically combined to between-genotype conservative epitope in FMDV structural proteinVP2 protein, the sensitivity and the specificity are high, the general foot-and-mouth disease virus structural protein antibody and the blocking ELISA detection reagent kit thereof are suitable for detection of structural protein antibodies after O, A and Asia1 type FMDV infected or inactivated vaccine immunity, and are a new method for monitoring FMD non-immune animal community infection conditions, and a complete set of identifying and diagnosing method is provided for a marker vaccine having FMDV structural protein VP2 epitope deletion.

The invention belongs to the technical field of plant protection, and discloses application of a specific sequence of a subspecies of leptosphaeria biglobosa to detection of the leptosphaeria biglobosa. The invention provides a specific detection target of L.biglobosa 'brassicae' (Lbb), and the target has the characteristics of good specificity, strong stability and high sensitivity, and can welldistinguish between a closely related species and the subspecies of the leptosphaeria biglobosa with relatively high homology, thereby avoiding the occurrence of false positives. An LAMP primer designed for the target can detect L.biglobosa 'brassicae' genomic DNA of 132fg / [mu]L, and provides a sensitive and rapid tool for the rapid detection and identification of Lbb, namely the subspecies of theleptosphaeria biglobosa.