Nasopharyngeal carcinoma marker and application thereof

A nasopharyngeal carcinoma and marker technology, which is applied to nasopharyngeal carcinoma markers and their application fields, can solve the problems of lack of trauma and high sensitivity, and achieves low price, high sensitivity and specific effects.

Active Publication Date: 2020-05-01
THE FIFTH AFFILIATED HOSPITAL SUN YAT SEN UNIV
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AI Technical Summary

Problems solved by technology

[0004] Aiming at the defect of the lack of less invasive and highly sensitive markers in the prior art, the present invention provides a nasopharyngeal carcinoma marker

Method used

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  • Nasopharyngeal carcinoma marker and application thereof

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Experimental program
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Effect test

Embodiment 1

[0023] Example 1 Comparison of the positive proportion of TREM-2 in peripheral blood CD8 lymphocytes and CD14 monocytes of nasopharyngeal carcinoma patients

[0024] 1. Collection of test samples: The peripheral blood of the subjects was collected, including 45 healthy people and 40 nasopharyngeal cancer patients.

[0025] 2. The detection method specifically includes the following steps:

[0026] (1) Use lymphocyte separation medium to separate PBMC from peripheral blood, take 1×10 5 ~1×10 7 cells, add 1 mL of PBS, and centrifuge at 1500 rpm for 5 minutes;

[0027] (2) Specific antibody staining reaction: 100 μL reaction system contains: PBMC is 1x10 5 ~1x10 7 cells, 100 μL of PBS, 3 μL of TREM-2 antibody or isotype control antibody IgG, 2 μL of CD14 antibody, and 2 μL of CD8 antibody, and the above reaction system was placed on ice (0-4°C) and incubated in the dark for 30 minutes. Add 1 mL of PBS, centrifuge at 1500 rpm for 5 minutes, repeat 3 times;

[0028] (3) Add 5...

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Abstract

The invention belongs to the technical field of immunotherapy, and particularly relates to a nasopharyngeal carcinoma marker and application thereof. In order to develop a nasopharyngeal carcinoma marker with small trauma and high sensitivity, the invention provides the nasopharyngeal carcinoma marker. The nasopharyngeal carcinoma marker is expressed in a lymphocyte of human peripheral blood; a healthy person and a nasopharyngeal carcinoma patient can be effectively distinguished through detecting the percentage of TREM-2 and CD8 double-positive cells in a CD8 single positive cell, the TREM-2on the surface of the lymphocyte is prompted to be expected to be applied to diagnosis of the nasopharyngeal carcinoma, the rapid detection on the nasopharyngeal carcinoma is realized, the accuracy rate can reach 95%, the nasopharyngeal carcinoma marker has the advantages of high sensitivity, good specificity, easy operation, easy acceptance of patients and the like, and is low in price and suitable for popularization and application.

Description

technical field [0001] The invention belongs to the technical field of immunotherapy, in particular to a nasopharyngeal carcinoma marker and its application. Background technique [0002] Nasopharyngeal carcinoma (NPC) is a polygenic hereditary disease and one of the most common head and neck malignancies in my country. It is related to tumor factors and has obvious racial aggregation and geographical distribution. Its pathogenesis is still unclear. According to the Global Tumor Statistics Report, the areas inhabited by Chinese in East Asia and Southeast Asia are high incidence areas of nasopharyngeal cancer. The main incidence areas in my country are located in Guangdong, Guangxi, Hong Kong and other southern provinces and cities. The annual incidence of nasopharyngeal cancer in these areas is about 20%. / 100000. The pathological types of nasopharyngeal carcinoma are mainly undifferentiated and poorly differentiated squamous cell carcinoma with high degree of malignancy. I...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/574
CPCG01N33/57407G01N33/57492G01N2333/70503G01N2333/70517
Inventor 黄曦吴永坚尹欢
Owner THE FIFTH AFFILIATED HOSPITAL SUN YAT SEN UNIV
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