Rapeseed black shank specific sequence and lamp detection primers and application

A technology for blackleg bacteria and rape, which is applied to the field of blackleg bacteria specific sequences and LAMP detection primers, can solve problems such as the specificity variation of amplification, and achieve the effects of controlling false positives, good specificity, and avoiding false positives
CN108949916BActive Publication Date: 2021-09-07HUAZHONG AGRI UNIV
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
HUAZHONG AGRI UNIV
Publication Date
2021-09-07

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Abstract

The invention belongs to the technical field of plant protection, and discloses the specific sequence of blackleg pathogen of rapeseed, LAMP detection primers and applications. The invention provides a specific detection target of L.biglobosa, and the target has good specificity, strong stability and high sensitivity. characteristics, it can distinguish the closely related species of Brassica napus with higher homology, avoiding the generation of false positives. The LAMP primers designed for this target can detect 112 fg / μL of L.biglobosa genomic DNA, which provides a good tool for field detection of Brassica napus and rapid high-throughput inspection and quarantine at ports.
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Description

technical field

[0001] The invention belongs to the technical field of plant protection, and in particular relates to the specific sequence of blackleg pathogen of rapeseed, a LAMP detection primer and application thereof. Background technique

[0002] Rapeseed blackleg (Blackleg) is a worldwide disease of rapeseed. According to reports, in each rapeseed production season, the global economic loss is as high as 900 million U.S. dollars (Fernando et al., 2016). The pathogen is the fungus of the genus Micrococcomus, which can seriously damage Brassicaceae plants such as rapeseed. Leptosphaeria fungi include two closely related species: Leptosp haeria maculans and L. biglobosa (Shoemaker and Brun., 2001) (Kaczmarek., 2009). The former is mainly distributed in Canada, Australia, Europe and other places, and the infection site is at the base of the rapeseed stem, which will cause the stem base to rot and cause the stem to fall, causing serious damage to rapeseed production and c...

Claims

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