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A method for rapidly immobilizing cells on a magnetic fixed bed

A technology of immobilized cells and magnetic fixed beds, applied in microorganism-based methods, biochemical equipment and methods, immobilized on or in inorganic carriers, etc. Eliminate the cumbersome operation of the elution and preparation process, achieve rapid immobilization and repeated use, have little effect on cell activity, and overcome the cumbersome process

Active Publication Date: 2022-02-08
HUAQIAO UNIVERSITY +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This traditional method of embedding and immobilizing cells has a series of disadvantages: 1. The process of collecting cells and washing cells is cumbersome; 2. When vigorously mixed with polymer materials, the cells are easily inactivated; 3. The size of the formed microcapsules is relatively small. Large, often have internal diffusion limitations; 4. The whole preparation process is cumbersome to operate, takes a long time, and the cost is high
[0003] At present, there are some methods of immobilizing cells by using magnetic fixed beds, but the traditional magnetic fixed beds mostly use ferromagnetic substances as magnetic media, such as iron powder, iron wire, steel wire, steel balls, etc. After the magnetic field is removed, there will still be residual magnetism, which will make it difficult to elute the superparamagnetic particles attached to the bed by the magnetic field, which seriously hinders the reuse of the magnetic fixed bed in immobilizing cells.

Method used

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  • A method for rapidly immobilizing cells on a magnetic fixed bed
  • A method for rapidly immobilizing cells on a magnetic fixed bed

Examples

Experimental program
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Effect test

Embodiment 1

[0032] 1) Preparation of superparamagnetic fluid: Mix 500mL ferric chloride solution with a concentration of 0.4mol / L and 500mL ferric chloride solution with a concentration of 0.2mol / L in equal volumes, stir vigorously, heat to 70°C, Quickly add 120mL of concentrated ammonia water (25-28%) to the reaction solution, continue to stir rapidly for 1 hour, magnetically separate after the reaction is completed, and remove the supernatant to obtain a superparamagnetic fluid;

[0033] 2) Preparation of superparamagnetic magnetic medium: take a hollow capillary glass tube with a length of 200mm, an inner diameter of 0.1mm, and a wall thickness of 0.1mm, fill the tube with the superparamagnetic fluid prepared in step 1), and move it down with the help of a permanent magnet Remove the dispersion liquid in the superparamagnetic fluid, fill the hollow capillary glass tube with superparamagnetic particles completely, after vacuum drying, sinter and close the two ends with an alcohol lamp to...

Embodiment 2

[0040] 1) Preparation of superparamagnetic fluid: Mix 500mL ferric chloride solution with a concentration of 0.4mol / L and 500mL ferric chloride solution with a concentration of 0.2mol / L in equal volumes, stir vigorously, and heat to 90°C. Quickly add 120mL concentrated ammonia water to the reaction solution, continue to stir rapidly for 1 hour, magnetically separate after the reaction is completed, remove the supernatant, and obtain a superparamagnetic fluid;

[0041] 2) Preparation of superparamagnetic magnetic medium: take a hollow capillary glass tube with a length of 500mm, an inner diameter of 0.2mm, and a wall thickness of 0.1mm, fill the tube with the superparamagnetic fluid prepared in step 1), and move it down with the help of a permanent magnet Remove the dispersion liquid in the superparamagnetic fluid, fill the hollow capillary glass tube with superparamagnetic particles completely, after vacuum drying, sinter and close the two ends with an alcohol lamp to form a su...

Embodiment 3

[0047] 1) Preparation of superparamagnetic fluid: Mix 500mL ferric chloride solution with a concentration of 0.4mol / L and 500mL ferric chloride solution with a concentration of 0.2mol / L in equal volumes, stir vigorously, heat to 70°C, Quickly add 120mL concentrated ammonia water to the reaction solution, continue to stir rapidly for 1 hour, magnetically separate after the reaction is completed, remove the supernatant, and obtain a superparamagnetic fluid;

[0048] 2) Preparation of superparamagnetic magnetic medium: take a hollow capillary glass tube with a length of 300mm, an inner diameter of 0.1mm, and a wall thickness of 0.1mm, fill the tube with the superparamagnetic fluid prepared in step 1), and move it down with the help of a permanent magnet Remove the dispersion liquid in the superparamagnetic fluid, fill the hollow capillary glass tube with superparamagnetic particles completely, and after vacuum drying, both ends are solidified and sealed with 502 glue to form a sup...

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Abstract

The invention discloses a method for rapidly immobilizing cells on a magnetic fixed bed, which has the advantages of simple process, low energy consumption, short time consumption, simple equipment, continuous operation, less influence on the activity of immobilized cells, and convenient loading and unloading of cells A series of advantages, such as biocatalysis and other fields have broad application prospects.

Description

technical field [0001] The invention belongs to the technical field of cell immobilization, in particular to a method for rapidly immobilizing cells in a fermentation broth, and in particular to a method for rapidly immobilizing cells on a magnetic fixed bed. Background technique [0002] Cell immobilization is an important method for reusing cells in whole-cell catalysis and fermentation processes, which is of great significance for improving production efficiency and saving costs in industrial processes. Common living cell immobilization methods mainly include adsorption and embedding methods. The cell density fixed by the adsorption method is small, so the embedding method has become the most commonly used method for living cell immobilization. The basic process of the embedding method is as follows: First, cells are collected from the fermentation broth by centrifugation, sedimentation or filtration, then the cells are washed to remove harmful metabolites on the surface,...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N11/14C12R1/19
CPCC12N11/14
Inventor 陈国柠檬赵珺郭洪伟陈宏文
Owner HUAQIAO UNIVERSITY
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