White palm seedling breeding method
A white palm and tissue culture seedling technology, applied in the field of plant seedling raising, can solve the problems of long-term lack of light, humidity sensitivity, and difficulty in flowering, and achieve the effects of strengthening mitochondrial activity, thick diameter, and developed root system.
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Embodiment 1
[0018] The method for breeding seedlings of Rhododendron, specifically comprises the following steps:
[0019] 1) Pick the seeds that are plump in shape and nearly intact in size, disinfect them with 1% sodium hypochlorite solution for 3 minutes, rinse them with distilled water, put them in an ice bath at 0°C for 2 hours to germinate, and the water contains 10g / L ethanol and 15mg / LN - Benzyl quinine chloride, absorb the water with absorbent paper, put a layer of filter paper and double gauze in the culture dish, put the seeds in the incubator for germination; the temperature of the incubator is 24°C, and the light time is 10h , the light intensity is 2000Lux, spray water every day, and stop spraying after germination; 2) Select a sponge with a length of 20cm, a width of 15cm, and a thickness of 0.5cm, wash it with distilled water and soak it in alcohol with a volume fraction of 75% for 30 minutes, and then rinse it with distilled water Wash and set aside; roll a piece of spong...
Embodiment 2
[0021] The method for breeding seedlings of Rhododendron, specifically comprises the following steps:
[0022] S1: Pick the seeds that are full in shape and close to intact in size, disinfect them with 2% sodium hypochlorite solution for 5 minutes, rinse them with distilled water, put them in an ice bath at 1°C for 3 hours to germinate, and the water contains 12g / L ethanol and 18mg / LN - Benzyl quinine chloride, dry the water with absorbent paper, put a layer of filter paper and double gauze in the culture dish, put the seeds in the incubator for germination; the temperature of the incubator is 26°C, and the light time is 12h , the light intensity is 2400Lux, spray water every day, and stop spraying after germination; adding a small amount of N-benzylquinine chloride and an appropriate amount of ethanol in the germination aqueous solution can help germination, under the action of an appropriate amount of ethanol, N-benzylquinone After the quinine chloride enters the seeds of th...
Embodiment 3
[0027] The method for breeding seedlings of Phyllostachys praecox, comprising: germination acceleration, induction culture, subculture multiplication culture, tissue culture seedling rooting, specifically includes the following steps:
[0028] Pregermination: pick the seeds that are full in shape and nearly intact in size, disinfect them with 2% sodium hypochlorite solution for 4 minutes, rinse them with distilled water, put them in an ice bath at 0°C for 2 hours, and the water contains 10g / L ethanol and 17mg / LN - Benzyl quinine chloride, absorb the water with absorbent paper, put a layer of filter paper and double gauze in the culture dish, put the seeds in the incubator for germination; the temperature of the incubator is 25°C, and the light time is 10h , the light intensity is 2200Lux, spray water every day, and stop spraying after germination; adding a small amount of N-benzylquinine chloride and an appropriate amount of ethanol in the germination aqueous solution can help ...
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