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A kind of microbial bacterial agent of Bacillus velei
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A microbial agent, bacillus technology, applied in the direction of microorganisms, microorganisms, biocides, etc., to achieve the effect of large spore production, various dosage forms, and convenient use methods
Active Publication Date: 2020-05-29
NORTH CHINA PHARMA GROUP AINO
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Bacillus Velez is a new species of Bacillus named by Ruiz-Garcfa in 2005. It is a new type of biocontrol bacteria. In recent years, relevant research has gradually increased. Some researchers have found that Bacillus Velez is effective against cucumber powdery mildew melons. Powdery mildew monofilamentia, Fusarium tritici, Fusarium solani, etc. have antagonistic activity, but there are few reports on Bacillus against peanut root rot
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preparation example Construction
[0043] Two, the preparation of the former powder of Bacillus Velez 1721
[0044] A variety of microbial preparations can be made from Bacillus veilei 1721 as the active ingredient. To prepare the bacterium for the preparation of microbial inoculum, it is necessary to prepare the original powder of Bacillus velesii 1721. The preparation steps of the original powder are as follows:
[0045] A. Inoculate Bacillus Velez 1721 into LB liquid medium, shake culture to bacterial density OD 600 The value is 0.6-0.8, and the strain is obtained;
[0046] The content of tryptone in the LB liquid medium is 9-11g / L, the content of yeast extract is 4-6g / L, the content of sodium chloride is 9-11g / L, and the pH value of the LB medium is 7.0-7.2;
[0047] B. Insert the bacterial classification in step A into the seed tank by 9~11% of the medium volume used in the seed tank, the cultivation temperature of the seed tank is 35~37°C, the tank pressure is 0.05~0.07MPa, and the ventilation ratio is 1...
Embodiment 1
[0073] The preparation steps of Bacillus 1721 granules are as follows:
[0074] Prepare granules according to the above steps, and the specific composition content and process parameters are shown in the following table:
[0078] The preparation steps of Bacillus Velez 1721 wettable powder are as follows:
[0079] Prepare the wettable powder according to the above steps, and the specific component content and process parameters are shown in the following table:
[0080] table 3
[0081]
[0082]
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Abstract
The invention discloses a microbial agent of Bacillus Velez, which belongs to the field of development and application of microorganisms. The Bacillus Velez is Bacillus Velez 1721, which has been published in Chinese microbial strains on January 29, 2018 It is registered in the General Microorganism Center of the Preservation Management Committee, and the deposit number is CGMCC No.15324. The Bacillus Velez 1721 provided by the present invention can be made into wettable powder, suspension concentrate, water-dispersible granule or granule, and has high antibacterial activity in various dosage forms, acts on peanuts, and can effectively control peanuts Root rot helps to reduce the amount of chemical agents used and residues in agricultural products, and has the advantages of safe use, no residue, and convenient use.
Description
technical field [0001] The invention relates to bacillus veles, in particular to a microbial agent for preventing and treating plant diseases. Background technique [0002] With the requirement of intensive agricultural production and sustainable development, crop diseases and insect pests have become the bottleneck restricting the sustainable development of modern agriculture. Various fungal diseases have caused a large number of crops and commercial crops to reduce or even fail to harvest, causing a lot of losses to the country and people's property. [0003] Peanut, also known as groundnut, belongs to the annual herb of Papilionaceae. Peanuts are known as "vegetable meat", with an oil content of up to 50%, high quality and a delicate smell. In addition to being edible, it is also used in printing and dyeing and papermaking industries. Peanuts are also a traditional Chinese medicine, suitable for malnutrition, spleen and stomach disorders, cough, phlegm, asthma, and lack...
Claims
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