A preparation method and application of an amperometric immunosensor using dual detection methods for mutual verification

An immunosensor, amperometric technology, used in nanotechnology for sensing, nanotechnology for materials and surface science, instruments, etc., can solve the problem that the detection method cannot explain the accuracy of the sensor, and achieve accurate quantification. The effect of detecting carcinoembryonic antigen, good conductivity, and accelerating electron transfer

Inactive Publication Date: 2020-02-14
SHANDONG UNIV OF TECH
View PDF10 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

A single detection method cannot account for the accuracy of the constructed sensor

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0046] Example 1 Preparation of Au@Pd NDs / Fe 2+ -CS / PPy NTs / Ab 2 include:

[0047] ① Preparation of gold-palladium core-shell nanodendrites (Au@Pd NDs)

[0048] Take 0.4 mL, 1.0 wt% chloroauric acid solution and add it to 49 mL of secondary water, heat it to boiling, quickly add 1.0 mL, 1.0 wt% sodium citrate solution, and keep boiling for 10 min under stirring conditions to obtain gold nanoparticles Particle dispersion: Add 80 mg of hexadecylpyridinium chloride, 8.0 mL of secondary water and 0.5 mL of 20 mmol / L palladium chloride solution to 15 mL of gold nanoparticle dispersion, and stir for 10 min Add 1.0 mL, 0.1 mol / L freshly prepared ascorbic acid solution, place at 40°C for 4 h, centrifuge, wash with water, and freeze-dry to obtain Au@Pd NDs;

[0049] ② Preparation of chitosan-functionalized polypyrrole nanotubes (CS / PPy NTs)

[0050] Take 0.2 g of ferric chloride and disperse it in 12 mL of secondary water, add 12 mL of 10 mmol / L methyl orange solution under stirrin...

Embodiment 2

[0055] Example 2 Preparation of Au@Pd NDs / Fe 2+ -CS / PPy NTs / Ab 2 include:

[0056] ① Preparation of gold-palladium core-shell nanodendrites (Au@Pd NDs)

[0057] Add 0.5 mL, 1.0 wt% chloroauric acid solution into 49 mL of secondary water, heat to boiling, quickly add 1.3 mL, 1.0 wt% sodium citrate solution, and keep boiling for 10 min under stirring conditions to obtain gold nanoparticles Particle dispersion: Add 85 mg of hexadecylpyridinium chloride, 8.0 mL of secondary water and 0.8 mL of 20 mmol / L palladium chloride solution to 18 mL of gold nanoparticle dispersion, and stir for 10 min Add 1.3 mL, 0.1 mol / L freshly prepared ascorbic acid solution, place at 40°C for 4 h, centrifuge, wash with water, and freeze-dry to obtain Au@Pd NDs;

[0058] ② Preparation of chitosan-functionalized polypyrrole nanotubes (CS / PPy NTs)

[0059] Take 0.25 g of ferric chloride and disperse it in 14 mL of secondary water, add 13 mL of 10 mmol / L methyl orange solution under stirring conditions...

Embodiment 3

[0064] Example 3 Preparation of Au@Pd NDs / Fe 2+ -CS / PPy NTs / Ab 2 include:

[0065] ① Preparation of gold-palladium core-shell nanodendrites (Au@Pd NDs)

[0066] Add 0.6 mL, 1.0 wt% chloroauric acid solution into 49 mL of secondary water, heat to boiling, quickly add 1.5 mL, 1.0 wt% sodium citrate solution, and keep boiling for 10 min under stirring conditions to obtain gold nanoparticles Particle dispersion: Add 90 mg of hexadecylpyridinium chloride, 8.0 mL of secondary water and 1.0 mL of 20 mmol / L palladium chloride solution to 20 mL of gold nanoparticle dispersion, and stir for 10 min Add 1.5 mL, 0.1 mol / L freshly prepared ascorbic acid solution, place at 40°C for 4 h, centrifuge, wash with water, and freeze-dry to obtain Au@Pd NDs;

[0067] ② Preparation of chitosan-functionalized polypyrrole nanotubes (CS / PPy NTs)

[0068] Take 0.3 g of ferric chloride and disperse it in 15 mL of secondary water, add 15 mL of 10 mmol / L methyl orange solution under stirring conditions,...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention belongs to the technical fields of novel nanomaterials, immune analysis and biosensing, and provides a preparation method and application of a current-type immune sensor that utilizes double detection methods to verify each other. Specifically, aminated graphene loaded with gold nanoparticles (Au NPs / NH 2 ‑GS) is the substrate platform, and the adsorption Fe of gold-palladium core-shell nanodendrites 2+ Chitosan-functionalized polypyrrole nanotube composites (Au@Pd NDs / Fe 2+ ‑CS / PPy NTs) as a sandwich amperometric immunosensor constructed as a marker. The constructed immunosensor uses the time-current curve method and square wave pulse voltammetry to detect different concentrations of carcinoembryonic antigen, and the mutual verification of the linear parts of the two detection results can improve the accuracy of the sensor, and it is the carcinoembryonic antigen The accurate detection of a reliable detection method.

Description

technical field [0001] The invention belongs to the technical fields of novel nanomaterials, immune analysis and biosensing, and provides a preparation method and application of a current-type immune sensor that utilizes dual detection methods to verify each other, and specifically relates to a gold-palladium-based core-shell nanodendrite Adsorption of Fe 2+ The chitosan-functionalized polypyrrole nanotube composite material is used to construct an amperometric immunosensor, and the application of the immunosensor in the detection of carcinoembryonic antigen by time-current curve method and square wave pulse voltammetry. Background technique [0002] According to the report of the World Health Organization in February 2018, about 8.8 million people died of cancer in the world in 2015. Chen Wanqing and others also mentioned in the article "Cancer statistics in China, 2015" that the total number of new cancer cases in China in 2015 is expected to be 4.292 million, and the exp...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
Patent Type & Authority Patents(China)
IPC IPC(8): G01N27/327G01N33/574B82Y30/00B82Y15/00
CPCB82Y15/00B82Y30/00G01N27/3277G01N27/3278G01N33/57473G01N2800/7028
Inventor 王平裴福彬马恩慧李明党余昊轩薛建强王瑞琛董云会李月云
Owner SHANDONG UNIV OF TECH
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap