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A special culture dish for cardiomyocytes and nerve cells

A technology for nerve cells and cardiomyocytes, which is applied in the cultivation devices of tissue cells/viruses, biochemical equipment and methods, biochemical instruments, etc. The amount of use, the effect of good light transmittance

Active Publication Date: 2022-02-01
太原市罗塞塔石生物技术有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The present invention aims to solve the technical problem of lacking a high borosilicate glass petri dish with simple pretreatment and reliable structure and strong adsorption force

Method used

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  • A special culture dish for cardiomyocytes and nerve cells
  • A special culture dish for cardiomyocytes and nerve cells
  • A special culture dish for cardiomyocytes and nerve cells

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] S1, in high-purity NH at a pressure of 5Pa 3 Under ambient conditions, vacuum plasma treatment for 10 minutes, and then vacuum freeze-drying for 1 minute;

[0031] S2, grafted O at a pressure of 30Pa 2 Under ambient conditions, vacuum plasma treatment for 4 minutes, and then vacuum freeze-drying for 10 minutes.

Embodiment 2

[0033] S1, high-purity NH at a pressure of 100Pa 3 Under ambient conditions, vacuum plasma treatment for 4 minutes, and then vacuum freeze-drying for 7 minutes;

[0034] S2, grafted O at a pressure of 70Pa 2 Under ambient conditions, vacuum plasma treatment for 7 minutes, and then vacuum freeze-drying for 1 minute.

Embodiment 3

[0036] S1, in high-purity NH at a pressure of 30Pa 3 Under ambient conditions, vacuum plasma treatment for 7 minutes, and then vacuum freeze-drying for 4 minutes;

[0037]S2, grafted O at a pressure of 5Pa 2 Under ambient conditions, vacuum plasma treatment for 1 min, and then vacuum freeze-drying for 7 min.

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Abstract

The invention discloses a special culture dish for cardiomyocytes and nerve cells, relates to the technical field of cell culture devices, in particular to a special culture dish for cardiomyocytes and nerve cells. The following scheme is adopted: it includes a barrel-shaped dish body, a through hole is vertically opened at the bottom of the dish body, and a high borosilicate glass is sealed and bonded to the lower end of the through hole, and the thickness of the high borosilicate glass is 0.13-0.175 mm, the dish body and high borosilicate glass are bonded and assembled and modified through the following two steps of pretreatment: the first step, the pressure is 5-100Pa of high-purity NH 3 environment, vacuum plasma treatment for 1-10min, then vacuum freeze-drying for 1-10min; the second step, in the pressure of 5-100Pa grafting O 2 Under ambient conditions, vacuum plasma treatment for 1-10min, then vacuum dry for 1-10min.

Description

technical field [0001] The invention relates to the technical field of cell culture devices, in particular to a special culture dish for cardiomyocytes and nerve cells. Background technique [0002] Cell culture is firstly the growth of single cells under in vitro conditions. It is widely used in biology, bioengineering, drug development, clinical and other fields. Now it has become one of the most effective methods in biology, medicine and pharmaceutical research. Cardiomyocytes cultured in vitro have the characteristics of spontaneous rhythm and contraction, making the culture of cardiomyocytes one of the basic methods and means for the study of heart diseases. The culture of nerve cells is of great significance to the treatment and research of damaged nerve tissue. [0003] In traditional cell culture methods, petri dishes generally use SiO 2 glass, SiO 2 Glass has strong adsorption to cells, but due to its poor light transmission, it is difficult to observe the growth...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12M3/00
CPCC12M23/02C12M23/20
Inventor 石鹏程张蓉王瑞
Owner 太原市罗塞塔石生物技术有限公司
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