Microbial compound inoculant for efficient degradation of grease, method and application thereof

A compound bacterial agent and microbial technology, applied in the field of microbial degradation, can solve the problem of low oil degradation efficiency and achieve the effect of small investment and high efficiency

Inactive Publication Date: 2018-12-18
SOUTH CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The purpose of the present invention is to overcome the defect of low efficiency of degradation of oil in oily wastewater by a single bacterial strain in the prior art, and provide a kind of microbial composite bacterial agent, by preferably compounding Bacillus licheniformis, Bacillus subtilis, proteolytic candida The ratio of yeast to Candida, and the optimal conditions for degrading oil can achieve the purpose of efficiently degrading oil

Method used

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  • Microbial compound inoculant for efficient degradation of grease, method and application thereof
  • Microbial compound inoculant for efficient degradation of grease, method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] Embodiment 1 A kind of microbial composite bacterial agent and preparation method thereof

[0031] A microbial compound bacterial agent, comprising the following bacterial species: bacillus licheniformis, bacillus subtilis, candida prions and candida. The quality ratio of each strain is as shown in Table 1:

[0032] The mass ratio of each strain in the microbial compound bacterial agent of table 1

[0033]

Bacillus licheniformis

Bacillus subtilis

Candida prionolyticus

Candida

Recipe 1

5

10

10

5

Recipe 2

5

15

15

10

Recipe 3

10

10

10

10

Recipe 4

10

15

15

10

[0034] The preparation method of the microbial composite bacterial agent comprises the following steps: S1. respectively inoculating Bacillus licheniformis and Bacillus subtilis in beef extract peptone medium, and shaking and culturing at 30°C for 48 to 72 hours; S2. Trichosaccharomyces and Candi...

Embodiment 2

[0038] The ability test of embodiment 2 microbial composite bacterial agent degrading grease under different conditions

[0039] According to the formula 3 of embodiment 1, the microbial composite bacterial agent was prepared and carried out the ability test of degrading grease under the following different conditions, and the concrete process is as follows:

[0040] 1. Optimizing the conditions for compound bacterial agent to degrade oil

[0041] After 48 hours of liquid culture for the test strains, they were compounded into seed liquids. Single factor test: Within 72 hours, different inoculum size, initial pH value, temperature and initial soybean oil content were used to detect the influence on the oil degradation rate, and the rotation speed was 150r / min. After 72 hours, samples were taken to measure the oil concentration, and the oil degradation rate was calculated.

[0042] 2. Measurement method

[0043] Take seven 25mL volumetric flasks, use soybean oil as the solut...

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Abstract

Belonging to the technical field of microbial degradation, the invention specifically discloses a microbial compound inoculant for efficient degradation of grease, a method and application thereof. The microbial compound inoculant comprises the following strains bacillus licheniformis, bacillus subtilis, proteolytic candida and candida in a mass ratio of 5-10:10-20:10-15:5-20. After inoculation ofthe microbial compound inoculant provided by the invention into oily sewage at an inoculation amount of 0.5-10% for reaction for 3 days, the grease degradation rate can reach 90% or more. The microbial compound inoculant provided by the invention can be applied to oily wastewater treatment and harmless treatment of culture wastes for resource utilization, can achieve large-scale industrial application, and has the advantages of small investment, high efficiency, no secondary pollution, etc.

Description

technical field [0001] The invention relates to the technical field of microbial degradation, in particular to a microbial composite bacterial agent and method for efficiently degrading oil and its application. Background technique [0002] With the continuous improvement of my country's economic level and living standards, the amount of oily wastewater discharged from oil processing plants and catering industries is increasing. Since grease is insoluble in water, the grease contained in sewage can easily lead to blockage of pipes. Direct discharge of oily wastewater will harm the aquatic ecosystem and seriously pollute the surrounding environment. [0003] Common treatment methods for oily wastewater mainly include physical, chemical and biological methods. At present, the most effective way to solve this problem is to let the sewage pass through the degreaser first, and then separate most of the grease in the sewage under the separation action of the degreaser. The sepa...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12N1/16C02F3/34C12R1/10C12R1/125C12R1/72C02F103/32
CPCC02F3/34C02F3/347C02F2103/322C12N1/16C12N1/20
Inventor 刘可星唐志远王雪玲
Owner SOUTH CHINA AGRI UNIV
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