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A screening method for germplasm resources of almond-scented tea tree

A screening method and tea tree technology are applied in the field of screening of almond fragrant tea tree germplasm resources, which can solve the problems of labor-intensive and time-consuming, slow screening speed, large workload, etc., and achieve easy popularization and use, easy implementation, and strong practicability. Effect

Active Publication Date: 2020-05-01
TEA RES INST GUANGDONG ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0002] "Almond Fragrance" black tea is black tea made from a special tea tree raw material. The finished tea has an almond milk aroma. It is extremely difficult to breed varieties with high-quality resources
[0003] The current screening method is to collect fresh leaves from a single plant or to process and trial-produce black tea after cutting and breeding a single plant, but these two methods have a huge workload and the screening speed is relatively slow
Using the method of collecting fresh leaves from a single plant for processing and trial production, due to the small number of tea greens in a single tea tree, processing is difficult, and the original characteristics of the germplasm resources cannot be maintained, so tea tree germplasm resources with certain characteristics cannot be screened, and The separate processing of each tea tree consumes a lot of manpower and time, and the efficiency is low. However, the method of using asexual cutting seedlings to cultivate and plant the cutting seedlings and then processing and trial production will take more than 4 years. This large-scale cutting breeding will eventually It is possible to screen thousands of copies of tea tree germplasm resources to produce "almond fragrance" varieties, but this method has the disadvantages of time-consuming, high cost, and heavy workload.

Method used

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  • A screening method for germplasm resources of almond-scented tea tree
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  • A screening method for germplasm resources of almond-scented tea tree

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Experimental program
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Effect test

Embodiment 1

[0023] The invention provides a technical scheme: a method for screening germplasm resources of almond-flavored tea tree, which is characterized in that it comprises the following steps:

[0024] S1. Raw material selection: select three tea tree raw materials, two of which have the "almond fragrance" tea tree germplasm qualification, and the other has no "almond fragrance" tea tree, and collect one bud and two leaves of the three tea trees;

[0025] S2. Sample grinding: Weigh 60 mg sample, put 360 μL of cold methanol and 40 μL of internal standard into a grinder for grinding, take it out, and ultrasonically extract it for 30 minutes;

[0026] S3. Sample extraction: add 200 μL of chloroform to the sample in S2, mix well, add water, mix well, and extract by ultrasonic for 30 minutes; The oximation reaction was carried out in a shaking incubator at 37°C, and the reaction time was 90 minutes. After the sample was taken out, it was placed at room temperature for 30 minutes;

[002...

Embodiment approach

[0037] The present invention provides another embodiment, a method for screening germplasm resources of almond-scented tea tree, which is characterized in that it comprises the following steps:

[0038] S1. Raw material selection: select three tea tree raw materials, two of which have the "almond fragrance" tea tree germplasm qualification, and the other has no "almond fragrance" tea tree, as a control;

[0039] S2. Sample grinding: Weigh 50 mg of sample, put it into a grinder and grind it, after taking it out, extract it by ultrasonic for 20 minutes;

[0040] S3. Sample extraction: add 100 μL of chloroform to the sample in S2, mix well, add water, mix well, and extract by ultrasonic for 10 min; Carry out the oximation reaction in a shaking incubator, take out the sample, and place it at room temperature for 30 minutes;

[0041] S4. Data analysis: collect sample data for GC-MS metabolomics analysis.

[0042] In step S1, the three tea tree samples were all collected in the st...

Embodiment 3

[0045] A method for screening almond-scented tea tree germplasm resources, characterized in that it comprises the steps of:

[0046] S1. Raw material selection: select three tea tree raw materials, two of which have the "almond fragrance" tea tree germplasm qualification, and the other has no "almond fragrance" tea tree, as a control;

[0047] S2. Sample grinding: Weigh 70 mg of sample, put it into a grinder and grind it, after taking it out, extract it by ultrasonic for 40 minutes;

[0048] S3. Sample extraction: add 300 μL of chloroform to the sample in S2, mix well, add water, mix well, and extract by ultrasonic for 130 min; Carry out the oximation reaction in a shaking incubator, take out the sample, and place it at room temperature for 30 minutes;

[0049] S4. Data analysis: collect sample data for GC-MS metabolomics analysis.

[0050] In step S1, the three tea tree samples were all collected in the state of one bud and two leaves; in step S2, 360 μL of cold methanol and ...

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Abstract

The invention discloses a method for screening almond-flavored tea tree germplasm resources. The method comprises the following steps of selecting three parts of tea raw materials, wherein the two parts have the almond-flavored tea tree germplasm resources, and the rest part does not have the almond-flavored tea tree germplasm resources; weighing 50-70 mg of a sample, putting the sample into a grinding machine for grinding, taking out the sample, and carrying out ultrasonic extraction for 20-40 minutes; adding 100-300 [mu]L of chloroform into the sample, performing uniform mixing, adding water, performing uniform mixing, and carrying out ultrasonic extraction for 10-30 minutes; adding a methoxyamine hydrochloride pyridine solution into a glass-derived small bottle, carrying out vortex oscillation for 2 minutes, carrying out oximation reaction in a concussion incubator, taking out the sample, and placing the sample at the room temperature for 30 minutes; and collecting sample data to perform GC-MS metabonomics analysis. Through the technical scheme, the content of mandelonitrile in tea leaf metabolites can be rapidly analyzed, so that the screening work of the almond-flavored tea tree germplasm resources can be rapidly realized, and the working time of original 4-year breeding is greatly shortened.

Description

technical field [0001] The invention relates to the technical field of tea planting, in particular to a method for screening germplasm resources of almond-flavored tea trees. Background technique [0002] "Almond Fragrance" black tea is black tea made from a special tea tree raw material. The finished tea has an almond milk aroma. It is extremely difficult to breed varieties with high quality resources. [0003] The current screening method is to collect fresh leaves from a single plant or to process and trial-produce black tea after cutting and breeding a single plant, but these two methods have a huge workload and the screening speed is relatively slow. Using the method of collecting fresh leaves from a single plant for processing and trial production, due to the small number of tea greens in a single tea tree, processing is difficult, and the original characteristics of the germplasm resources cannot be maintained, so tea tree germplasm resources with certain characteris...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N30/02G01N30/06
CPCG01N30/02G01N30/06
Inventor 姜晓辉吴华玲方开星陈栋王秋霜秦丹丹李红建
Owner TEA RES INST GUANGDONG ACAD OF AGRI SCI
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