Kit for detecting anti-psychosis drugs in serum and plasma by liquid chromatography tandem mass spectrometry method and application thereof

Abstract

The invention provides a kit for detecting anti-psychosis drugs in a serum and plasma by a liquid chromatography tandem mass spectrometry method. The kit comprises drug standards: amsulpiride, aripiprazole, dehydroaripiprazole, chlorpromazine, clozapine, n-desmethylclozapine, risperidone, 9-hydroxyrisperidone, quetiapine, olanzapine, ziprasidone; drug internal standard compounds: amplepiride-d5, aripiprazole-d8, chlorpromazine-d3, clozapine-d8, risperidone-d4, 9-hydroxyrisperidone-d4, quetiapine-d8, olanzapine-d8, ziprasidone-d8; drug extraction compounds: methanol solution 60% (volume ratio),acetonitrile solution 20%, isopropanol solution 10%, purified water 10%; negative plasma; and a diluent: 50% aqueous methanol solution. The kit can be used to simultaneously detect anti-psychosis drugs and active metabolites thereof, the detection time is short and the flux is large.

Description

technical field [0001] The invention relates to the technical field of medicine, in particular to a kit for detecting anti-schizophrenic drugs in serum plasma by liquid chromatography tandem mass spectrometry and its application. The kit is suitable for detecting anti-schizophrenic drugs in serum plasma by using tandem mass spectrometry . Background technique [0002] Schizophrenia (schizophrenia) is a very stubborn and painful mental illness with a large number of patients. At present, schizophrenia control drugs are used clinically to control schizophrenia. However, although anti-schizophrenia drugs do not belong to the category of drugs at the legal level, they can affect people's psychological processes such as thinking, emotion, and will, and then change individuals. Long-term use will lead to drug resistance and dependence. Excessive use can easily cause poisoning, and severe cases can lead to death. Therefore, when patients use this antipsychotic drug, it is necessa...

AI Technical Summary

Problems solved by technology

[0004] At present, there have been certain developments in the detection of psychotropic drugs by liquid chromatography tandem mass spectrometry on the market. For example, Chinese application number CN 107807178A discloses a "liquid chromatography-mass spectrometry detection method for drugs and antipsychotic drugs in blood", using In the simultaneous detection of 9 kinds of drugs and 14 kinds of antipsychotic drugs, another example in 2010 Zhang Xiuyao et al. ("Ultra-high performance liquid chromatography-tandem mass spectrometry simultaneously and rapidly confirmed the detection of 42 psychotropic drugs and their metabolites in plasma and urine" , Chromatography, 2010, 28(1): 23‐33) proposed an ultra-high performance liquid chromatography‐tandem mass spectrometry method for the simultaneous rapid detection of 42 psychotropic drugs and their metabolites in plasma and urine. Drugs include antidepressants, antiepileptic drugs, antipyretic and analgesic drugs, etc. However, the detection methods of the prior art have the following disadvantages: Although there are many types of drugs detected, the detection accuracy is often uncontrollable and very vulnerable to The influence of matrix effect, and the detection time is long and the efficiency is low, which cannot meet the needs of clinical high-throughput sample detection

In addition, most of the current detection methods only detect the concentration of specific drugs and ignore the situation of their active metabolites, thus limiting the application of antipsychotic drug detection

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