Application and application of reagents for detecting serum exosome pigr in the preparation of kits for diagnosing primary biliary cholangitis and predicting its curative effect

Abstract

The invention belongs to the field of medical detection, and relates to a serum exosome PIGR, specifically to a use for using serum exosome pIgR as a marker for diagnosing primary biliary cholangitisand predicting curative effect. The technical solution adopted by the invention is a kit for using serum exosome pIgR as a marker for diagnosing primary biliary cholangitis and predicting curative effect, which is characterized by including the following components: a pIgR standard, a polystyrene ELISA (Enzyme Linked Immunosorbent Assay) microplate coated with pIgR antigens, a second enzyme-labeled antibody, a sample diluent, and an auxiliary reagent. The technical solution of the application method of the kit includes the following steps of: (1) preparing the sample diluent; (2) diluting thepIgR standard with a BSA-PBS solution into 8 gradients; (3) adding the standard and the sample diluent to a microplate; (4) adding the second enzyme-labeled antibody to each well; (5) adding a color developing liquid to each well; and (6) using a microplate reader to read an OD value at a wavelength of 450 nm , drawing a standard curve, and converting pIgR concentration in the sample. New kits andapplications are implemented by employing the above schemes.

Description

technical field [0001] The invention belongs to the field of medical detection, and relates to a serum exosome PIGR, specifically, the use of serum exosome pIgR as a diagnostic marker for primary biliary cholangitis and to predict curative effect. Background technique [0002] Primary biliary cholangitis (PBC), formerly known as primary biliary cirrhosis (primary biliary cirrhosis), is a chronic progressive autoimmune disease involving the intrahepatic bile duct system, mainly manifested as liver Progressive destruction of the small internal bile ducts with inflammatory changes in the portal vein eventually leads to fibrosis, cirrhosis and even liver cancer. The disease mainly occurs in middle-aged women, and only 10% of male cases. At present, the anti-mitochondrial antibody and its M2 subtype in serum are the most important auxiliary diagnostic indicators for PBC, but there are a certain proportion of missed and misdiagnosed indicators in this indicator, and it is impossi...

AI Technical Summary

Problems solved by technology

[0007] The present invention mainly solves the problems in the prior art that the methods for diagnosing primary biliary cholangitis or predicting the therapeutic effect of ursodeoxycholic acid on primary biliary cholangitis are limited and the effect is not good. Kit and application of secretory PIGR as a marker for the diagnosis and prediction of curative effect of primary biliary cholangitis

Images