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Application and application of reagents for detecting serum exosome pigr in the preparation of kits for diagnosing primary biliary cholangitis and predicting its curative effect

A technology of exosomes and kits, applied in the field of medical detection, can solve the problems of limited means and poor effect.

Active Publication Date: 2020-08-25
THE FIRST AFFILIATED HOSPITAL OF WENZHOU MEDICAL UNIV
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Problems solved by technology

[0007] The present invention mainly solves the problems in the prior art that the methods for diagnosing primary biliary cholangitis or predicting the therapeutic effect of ursodeoxycholic acid on primary biliary cholangitis are limited and the effect is not good. Kit and application of secretory PIGR as a marker for the diagnosis and prediction of curative effect of primary biliary cholangitis

Method used

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  • Application and application of reagents for detecting serum exosome pigr in the preparation of kits for diagnosing primary biliary cholangitis and predicting its curative effect
  • Application and application of reagents for detecting serum exosome pigr in the preparation of kits for diagnosing primary biliary cholangitis and predicting its curative effect
  • Application and application of reagents for detecting serum exosome pigr in the preparation of kits for diagnosing primary biliary cholangitis and predicting its curative effect

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Embodiment Construction

[0032] Application of a reagent for detecting serum exosome pIgR in the preparation of a kit for diagnosing primary biliary cholangitis and predicting its curative effect, the kit includes the following components: pIgR standard; pIgR antigen package coated polystyrene ELISA microplate; enzyme-labeled secondary antibody: horseradish peroxidase (HRP)-labeled anti-human IgG; sample diluent: BSA-PBS solution with a mass concentration of 0.1%; auxiliary reagent: substrate detector color solution, reaction stop solution and wash buffer.

[0033]In this embodiment, the sample diluent is bovine serum albumin added to the 0.01M phosphate buffer solution of pH7.4 and fully dissolved, and 1 gram of bovine serum albumin is added in every liter of phosphate buffer solution; The color solution is TMB / H2O2, the reaction termination solution is: 0.5M sulfuric acid, and the washing buffer solution is Tween20-PBS solution with a mass concentration of 0.05%. The substrate chromogenic solution ...

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Abstract

The invention belongs to the field of medical detection, and relates to a serum exosome PIGR, specifically to a use for using serum exosome pIgR as a marker for diagnosing primary biliary cholangitisand predicting curative effect. The technical solution adopted by the invention is a kit for using serum exosome pIgR as a marker for diagnosing primary biliary cholangitis and predicting curative effect, which is characterized by including the following components: a pIgR standard, a polystyrene ELISA (Enzyme Linked Immunosorbent Assay) microplate coated with pIgR antigens, a second enzyme-labeled antibody, a sample diluent, and an auxiliary reagent. The technical solution of the application method of the kit includes the following steps of: (1) preparing the sample diluent; (2) diluting thepIgR standard with a BSA-PBS solution into 8 gradients; (3) adding the standard and the sample diluent to a microplate; (4) adding the second enzyme-labeled antibody to each well; (5) adding a color developing liquid to each well; and (6) using a microplate reader to read an OD value at a wavelength of 450 nm , drawing a standard curve, and converting pIgR concentration in the sample. New kits andapplications are implemented by employing the above schemes.

Description

technical field [0001] The invention belongs to the field of medical detection, and relates to a serum exosome PIGR, specifically, the use of serum exosome pIgR as a diagnostic marker for primary biliary cholangitis and to predict curative effect. Background technique [0002] Primary biliary cholangitis (PBC), formerly known as primary biliary cirrhosis (primary biliary cirrhosis), is a chronic progressive autoimmune disease involving the intrahepatic bile duct system, mainly manifested as liver Progressive destruction of the small internal bile ducts with inflammatory changes in the portal vein eventually leads to fibrosis, cirrhosis and even liver cancer. The disease mainly occurs in middle-aged women, and only 10% of male cases. At present, the anti-mitochondrial antibody and its M2 subtype in serum are the most important auxiliary diagnostic indicators for PBC, but there are a certain proportion of missed and misdiagnosed indicators in this indicator, and it is impossi...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N33/543G01N33/531
CPCG01N33/531G01N33/543G01N2800/08
Inventor 陈永平许烂漫吴发玲
Owner THE FIRST AFFILIATED HOSPITAL OF WENZHOU MEDICAL UNIV
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