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Method for identifying closeness of genetic relationships of pumpkins

A technology of kinship and pumpkin, which is applied in the field of identifying the distance of kinship of pumpkins, can solve the problems that it is difficult to accurately identify the distance of kinship of pumpkins, and achieve the effect of convenient operation and intuitive map

Inactive Publication Date: 2019-01-04
FOSHAN UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] In order to solve the problem in the prior art that it is difficult to accurately identify the close relationship of pumpkins through plant morphological characteristics, the present invention provides a method for identifying the close relationship of pumpkins

Method used

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  • Method for identifying closeness of genetic relationships of pumpkins
  • Method for identifying closeness of genetic relationships of pumpkins

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] The method for constructing the general map of enzyme electrophoresis of pumpkins from each known place of origin includes:

[0033] (A) Take 1 g of pumpkin root, add 3 mL of phosphate buffer solution with a concentration of 0.05 mol / L, homogenize in a pre-cooled mortar and centrifuge, take the supernatant, add glycerin and shake well to obtain the enzyme electrophoresis solution and store it at low temperature ;

[0034] (B) using a gel electrophoresis system to perform electrophoresis on the enzyme electrophoresis solution described in step (A);

[0035] (C) Mapping the pattern and counting the number of bands based on the results of step (B).

[0036] The pH of the phosphate buffer described in the step (A) is 6.8, the added amount of glycerol is 20 (wt)%, and the temperature for cryopreservation is -20°C; the gel electrophoresis system described in the step (B) is a discontinuous acrylamide gel Gel electrophoresis system; the electrophoresis is steady flow electro...

Embodiment 2

[0038] The method for constructing the general map of enzyme electrophoresis of pumpkins from each known place of origin includes:

[0039] (A) Take 1 g of pumpkin stem, add 3 mL of phosphate buffer solution with a concentration of 0.05 mol / L, homogenize it in a pre-cooled mortar and centrifuge, take the supernatant, add glycerin and shake well to obtain the enzyme electrophoresis solution and store it at low temperature ;

[0040] (B) using a gel electrophoresis system to perform electrophoresis on the enzyme electrophoresis solution described in step (A);

[0041] (C) Mapping the pattern and counting the number of bands based on the results of step (B).

[0042] The pH of the phosphate buffer described in the step (A) is 6.8, the added amount of glycerol is 20 (wt)%, and the temperature for cryopreservation is -20°C; the gel electrophoresis system described in the step (B) is a discontinuous acrylamide gel Gel electrophoresis system; the electrophoresis is steady flow elec...

Embodiment 3

[0044] The method for constructing the general map of enzyme electrophoresis of pumpkins from each known place of origin includes:

[0045] (A) Take 1 g of pumpkin leaves, add 3 mL of phosphate buffer solution with a concentration of 0.05 mol / L, homogenize in a pre-cooled mortar and centrifuge, take the supernatant, add glycerin and shake well to obtain the enzyme electrophoresis solution and store it at low temperature ;

[0046] (B) using a gel electrophoresis system to perform electrophoresis on the enzyme electrophoresis solution described in step (A);

[0047] (C) Mapping the pattern and counting the number of bands based on the results of step (B).

[0048] The pH of the phosphate buffer described in the step (A) is 6.8, the added amount of glycerol is 20 (wt)%, and the temperature for cryopreservation is -20°C; the gel electrophoresis system described in the step (B) is a discontinuous acrylamide gel Gel electrophoresis system; the electrophoresis is steady flow elect...

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Abstract

The invention provides a method for detecting production places of pumpkins. The method includes constructing enzyme electrophoresis general patterns of different pumpkins from known production places; establishing enzyme electrophoresis patterns of the pumpkins from the to-be-detected production places by the aid of identical processes; carrying out clustering analysis on the enzyme electrophoresis patterns and the enzyme electrophoresis general patterns; computing pattern difference coefficients C to obtain the closeness of genetic relationships of the to-be-detected pumpkins and the known pumpkins. The mobility Rf of the patterns is higher than 0.2, the difference coefficients C meet an equation of C=2*w / (a+b), the w represents the numbers of enzyme strips of a type of the pumpkins fromthe to-be-detected production places and the pumpkins from the known production places, the types of the enzyme strips with the numbers represented by the w are different from one another as shown bymeans of comparison in pair, and the (a+b) represents the total numbers of enzyme strips of the respective types compared in pair. The method for identifying the closeness of the genetic relationships of the pumpkins has the advantages that the method is convenient to implement, the patterns are intuitive, and the closeness of the genetic relationships of the to-be-detected pumpkins and the knownpumpkins can be objectively and accurately reflected; the method is free of restraints of the morphology, the sizes and the growth time of the pumpkins, and different positions of the pumpkins can beused for identification.

Description

technical field [0001] The invention belongs to the technical field of pumpkin breeding, and in particular relates to a method for identifying the close relationship of pumpkins. Background technique [0002] Pumpkin (scientific name: Cucurbita moschata (Duch.ex Lam.) Duch.ex Poiret) is a species of Cucurbita moschata (Duch.ex Lam.) Duch.ex Poiret, an annual vine herb, with stems often rooting at nodes, thick petioles, broad oval or oval leaves, Slightly soft, with raised veins, slightly thick tendrils, monoecious, strong fruit stems, with ribs and grooves, which vary from variety to variety, and there are often several longitudinal grooves outside, many seeds, long oval or oblong, with good food therapy effect. Pumpkins are native to Mexico and Central America, and are widely cultivated all over the world, with the largest cultivated area in Asia, followed by Europe and South America, and generally cultivated in China. [0003] Pumpkin cultivars are rich in resources. Onl...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/447
CPCG01N27/447
Inventor 何丽烂任吉君王惠珍区炳庆
Owner FOSHAN UNIVERSITY
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