Novel anti-human IgG1-Fc antibody reagent and preparation method thereof

Abstract

The invention discloses a novel anti-human IgG1-Fc antibody reagent and a preparation method thereof, which relate to the technical fields of antigen detection. The anti-human IgG1-Fc antibody reagentcomprises a plurality of antibodies; among the plurality of antibodies, any two antibodies have different variable regions and a same constant region, or any two antibodies have different variable regions and different constant regions, and different antibodies bind to different antigenic epitopes of the human IgGl-Fc antigen. The use of the antibody reagent can avoid the result of non-response detection of mutation of IgG1-Fc antigen at different positions, improves the success rate and accuracy of the detection result, and avoids false negative results.

Description

technical field [0001] The invention relates to the technical field of antigen detection, in particular to a novel anti-human IgG1-Fc antibody reagent and a preparation method thereof. Background technique [0002] Antibody reagents used in the field of life sciences are divided into monoclonal antibody reagents and polyclonal antibody reagents. [0003] The preparation of monoclonal antibody reagents and polyclonal antibody reagents is well known to those skilled in the art. [0004] One of the classic methods for the preparation of monoclonal antibody reagents is: first immunize animals with antigens, then fuse the B cells of the immunized animals with tumor cells, form hybridoma cells, and further screen to obtain positive monoclonal hybridoma cell lines , use positive hybridoma cells to prepare ascites, and obtain a large amount of monoclonal antibodies through ascites purification; or analyze the antibody gene sequence of positive hybridoma cell lines, construct antibo...

AI Technical Summary

Problems solved by technology

[0006] The disadvantages of monoclonal antibody reagents include but are not limited to the following: the recognition of epitopes is single, the sensitivity is not as good as that of polyclonal antibodies, and the epitopes are easily affected by the physical and chemical properties during the experiment, which often leads to changes in epitopes, making monoclonal antibody reagents Unrecognized or reduced sensitivity

[0007] The disadvantages of polyclonal antibody reagents include but are not limited to the following: poor specificity, often non-specific reactions and cross-reactions

The composition is complex, standardized production is difficult, and there are differences between production batches, etc.

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