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A method for detecting signal of diabetic nephropathy and glomerular mesangial cells utilizing Dendrobium officinale

A technology of diabetic nephropathy and Tiepi Fengdou, which is applied in the direction of urinary system diseases, disease diagnosis, metabolic diseases, etc., can solve the problems of many side effects, high price, and difficulty in finding kidney sources, and achieve the reduction of expression level, MDA level, kidney Mild effects of pathological changes

Inactive Publication Date: 2019-01-15
SHANGQIU MEDICAL COLLEGE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the treatment after the onset of ND includes: drug control of hypertension and hyperglycemia, blood purification, stem cell transplantation, kidney transplantation, etc. The hot spots are transplantation immunology research, kidney preservation, diagnosis and prevention of rejection, but these There are many shortcomings in the treatment, such as many side effects, high price, and difficulty in finding kidney sources.

Method used

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  • A method for detecting signal of diabetic nephropathy and glomerular mesangial cells utilizing Dendrobium officinale
  • A method for detecting signal of diabetic nephropathy and glomerular mesangial cells utilizing Dendrobium officinale
  • A method for detecting signal of diabetic nephropathy and glomerular mesangial cells utilizing Dendrobium officinale

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0057] Effects of DC on Physiological and Biochemical Indexes of Rats

[0058] The weight of the rats in the diabetic model group was significantly lower than that of the normal control group (P<0.01), and the weight of the rats in the DC treatment group increased compared with the model group (P<0.05). Compared with the normal control group, the kidney weight index of rats in the model control group was significantly increased (P<0.01), and the kidney weight index of all groups were significantly decreased after DC treatment (P<0.01). Compared with the normal group, the blood Scr, blood BUN and 24hUP of all diabetic model rats were significantly increased; after DC treatment, these biochemical indicators were significantly reduced, as shown in the table below.

[0059] Table 1 Comparison of changes in clinical and biochemical parameters between groups

[0060]

[0061] ## P* P** P<0.01 vs DM group

Embodiment 2

[0063] Expression of MDA-1 and SOD in rat renal cortex

[0064] Compared with the normal group, the content of MDA-1 in the renal cortex of rats in the DM group was significantly increased (P image 3 shown.

Embodiment 3

[0066] Pathological changes of rat kidney

[0067] The results of HE staining showed that the normal control group had no obvious pathological changes in the glomerular structure; the model control group had significant pathological changes in the kidneys, with hypertrophy of the glomeruli, proliferation of mesangial cells, increased mesangial matrix, and basal Membrane thickening; the above-mentioned pathological changes in the DC group were significantly alleviated after treatment, and it was concentration-dependent, such as Figure 4 shown.

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Abstract

The invention belongs to the technical field of traditional Chinese medicines, and discloses a method for detecting signal of diabetic nephropathy and glomerular mesangial cells utilizing Dendrobium officinale. Streptozotocin is adopted for induction to build a diabetic rat model, and the Dendrobium officinale particles with different dosages are administrated for 8 weeks, and changes in biochemical indexes and renal pathology of rats of each group are observed. HMC is cultured in vitro and added with normal concentration of glucose, mannitol, high glucose and different concentrations of Dendrobium officinale. Activity of p-38MAPK and downstream TGF-beta and CTGF expression are observed. The invention establishes the diabetic rat model, and uses different concentrations of Dendrobium officinale for treatment, the Dendrobium officinale can obviously reduce TGF-beta and CTGF expression levels in the HMC cells, and the Dendrobium officinale can achieve an anti-fibrosis effect through influencing the activity of the p-38MAPK signaling pathway to regulate downstream TGF-beta and CTGF expression.

Description

technical field [0001] The invention belongs to the technical field of traditional Chinese medicine detection, and in particular relates to a signal detection method of Tiepi Fengdou for diabetic nephropathy and glomerular mesangial cells. Background technique [0002] At present, the existing technology commonly used in the industry is as follows: diabetic nephropathy is one of the main causes of diabetes death, high sugar is an important pathogenic factor of diabetic nephropathy, the main symptom of diabetic nephropathy is fibrosis, p38 mitogen The activated protein kinase (p38MAPK) signal transduction pathway is a common pathway for intracellular signal transmission, transforming growth factor β (TGF-β) and connective tissue growth factor (CTGF) are important pro-fibrotic factors, in diabetic nephropathy (DN) Fibrosis plays an important role. Currently, the treatment of diabetic nephropathy mainly relies on western medicine to control it. Western medicine has relatively l...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/8984A61P3/10A61P13/12A61K31/7008G01N33/569G01N33/68A01K67/027
CPCA01K67/027A01K2267/0362A61K31/7008A61K36/8984A61P3/10A61P13/12G01N33/5044G01N33/56966G01N33/6863G01N2333/495G01N2800/042G01N2800/347
Inventor 常景芝陈剑黄宇涵李宜川田华芦琨宋玮刘国玲沈永杰尤丽菊郭辉郭亚莉刘青
Owner SHANGQIU MEDICAL COLLEGE
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